Molecular Formula | C33H40O15 |
Molar Mass | 676.66 |
Density | 1.55 |
Melting Point | 223-225 ºC |
Boling Point | 948.5±65.0 °C(Predicted) |
Specific Rotation(α) | D15 -87.09° (in pyridine) |
Flash Point | 300.9°C |
Solubility | Soluble in ethanol, ethyl acetate, insoluble in water, insoluble in ether, benzene, chloroform. |
Vapor Presure | 0mmHg at 25°C |
Appearance | Pale yellow needle crystalline powder |
Color | light yellow to yellow |
Maximum wavelength(λmax) | ['350nm(MeOH)(lit.)'] |
Merck | 14,3617 |
pKa | 5.90±0.40(Predicted) |
Storage Condition | 2-8°C |
Stability | Light Sensitive |
Refractive Index | 1.679 |
MDL | MFCD00210516 |
Physical and Chemical Properties | Soluble in ethanol, ethyl acetate, insoluble in water, insoluble in ether, benzene, chloroform. It is derived from the plant Epimedium brevicornum Maxim. |
Safety Description | 24/25 - Avoid contact with skin and eyes. |
WGK Germany | 3 |
RTECS | DJ2980500 |
HS Code | 29389090 |
light yellow needle Crystal (hydrous pyridine),mp 231-232 °c. [Α] D22-92 °. Soluble in ethanol, ethyl acetate, insoluble in ether, benzene, chloroform.
0.5g of epimedium herb powder or an appropriate amount of extract was added with 10ml of ethanol, and the mixture was incubated for 30 minutes, filtered, the filtrate was evaporated to dryness, and the residue was dissolved by adding 1ml of ethanol, which was the sample solution. Icariin control was weighed, dissolved in methanol and diluted to 0.1mg/ml, which was the control solution. 10 μl of the test solution and 10 μl of the reference solution were picked up on the silica gel H thin layer plate with the same sodium carboxymethyl cellulose as the adhesive, and ethyl acetate-methyl ethyl ketone-formic acid-water (10:1:1:1) for the development of the solvent, expand, take out, dry, set the ultraviolet lamp (365nm) to view, and the same dark red spots are displayed on the corresponding position of the chromatogram of the test sample; the sample was sprayed with aluminum trichloride solution and inspected by ultraviolet lamp (365nm), showing the same orange-red fluorescent spots.
The reference substance icariin was accurately weighed and prepared into a standard solution of 10ug/ml with methanol. Accurately weigh the appropriate amount of this product powder (equivalent to about 1mg of icariin), ultrasonic with 25ml of methanol for 30 minutes, set the volume in a 50ml measuring flask, and filter with a 0.45 μm microporous filter membrane, that is, the test solution. Take the test solution and the reference solution respectively, take the reagent as the blank, measure the absorbance at 270nm wavelength, and calculate it.
chromatographic conditions and system suitability test: ODS2 5u C18 (4mm × 150mm) column; Mobile phase: acetonitrile-water (30:70); Detection wavelength: 270nm; Flow rate: 0.8/min. Theoretical plate number icariin is not less than 1500.
The extract of herba epimedii can promote the function of gonad. Flavonoids such as icariin have the function of tonifying kidney and strengthening Yang, 30~1000 μg/l can promote the secretion of estradiol by follicular granulosa cells, in 1000g/l can promote the secretion of corticosterone in adrenal cortex cells. At the same time, EPS can increase the level of gonadal hormone, mainly increase the level of FSH.
50% methanol extract of epimedium pubescens could inhibit lymphocyte transformation. Total flavonoids of herba epimedii can make the "yang deficiency" mice form cell function and antibody is high, significantly enhance the immune function of human T cells. Both EPS and EI have immunomodulatory effects.
both EPS and EI have antioxidant activity, which can enhance the activity of antioxidant enzyme and reduce the effect of free radical products.
herba epimedii extract can affect the cell passage, prolong the growth period, regulate the immune and secretion system, improve the metabolism and function of various organs and prolong life.
The non-amino acids in the extract of epimedium alcohol could significantly increase the coronary flow of isolated rabbit heart. Decoction and alcohol infusion can lower blood pressure. Icariin can directly relax vascular smooth muscle, can expand coronary artery, femoral artery and cerebral artery. The mechanism of action is to inhibit the influx of extracellular calcium ions in vascular smooth muscle cells.
In Vitro Test, epimedium has strong inhibitory effect on Staphylococcus aureus and Staphylococcus aureus. The inhibition of spinal cord gray matter in tissue culture of Virus, E-CHO Virus and Coxsackie Virus Virus. The subcutaneous injection of methanol extract of herba epimedii can significantly inhibit the egg white foot swelling of rats, and can reduce the increase of capillary permeability induced by histamine in rabbits.
The experimental hyperlipidemia rabbits were given Epimedium decoction for 10 days, which had the effect of reducing the cholesterol and triglyceride of B. It has obvious hypoglycemic effect on experimental hyperglycemic rats after oral administration of epimedium extract.
total flavonoids of epimedium can significantly improve the phagocytic function of macrophages and the transformation rate of T lymphocytes in tumor-bearing mice, these results suggest that it can promote the nonspecific killing of tumor cells by macrophages and the specificity and cytotoxicity of T lymphocytes, and has a certain recovery effect on the low cellular immune function of tumor bearing mice, at the same time can increase the spleen body ratio, significantly improve the erythrocyte C3b receptor rosette rate and reduce the erythrocyte immune complex rosette rate. These results suggest that total flavonoids of epimedium can increase the cellular immune function and erythrocyte immune function of tumor-bearing mice, and inhibit the growth of tumor cells to a certain extent.
The pharmacological effects related to the efficacy of epimedium for tonifying kidney yang and strengthening bones are to enhance gonadal function, regulate immune function, promote bone growth, delay aging and so on. Epimedium also has strong heart, lowering blood pressure, anti-arrhythmia, increase cerebral blood flow, antibacterial, anti-viral, anti-inflammatory, hypolipidemic, anti-tumor and other effects, with high medicinal and health value. However, the research and development is still in the initial stage, and the research on the effective components of Epimedium is not deep enough, and the microscopic mechanism of pharmacological effects observed on the macro level needs to be further studied. Therefore, to make use of the advantages of China's Epimedium resources, the use of traditional Chinese medicine experience combined with modern science and technology, in its effective ingredients, preparation process, pharmacological effects and clinical application of in-depth study, epimedium may become a good development prospect of enhancing immune function and immune regulation, anti-osteoporosis, treatment of cardiovascular and cerebrovascular diseases, it has broad application prospects.
icariin can increase cardiovascular and cerebrovascular blood flow, promote hematopoietic function, immune function and bone metabolism, with kidney yang, anti-aging, anti-tumor effect.