Storage Condition | RT, dark, 12 months |
Use | This product is for scientific research only and shall not be used for other purposes. |
Reference Show more | 1. Xiunfeng Han, Haitao Li, Rongya Yang, et al. Effects of in vitro and in vivo induction on the morphology and drug sensitivity of asaxi trichosporidia [J]. Journal of Practical Dermatology: 2015 000(001):6-10. 2. Meng Jie, Li Hongxia, Luo Xuemei, Chen Xiufen, Liu Chunsheng, Yang Yao-Hui. Study on the diversity of fungi on the surface of earthworm [J]. Chinese Journal of Traditional Chinese Medicine, 2019,44(20):4433-4438. 3. Li Hongxia, Xu Qing, Zhang Fan, et al. Identification of surface fungi of Bombyx batryticatus by three strategies based on colony morphology, microscopic characteristics and DNA barcoding [J]. World Science and Technology-modernization of traditional Chinese medicine, 2018, 020(007):1256-1260. 4. An Xizhong, Li Junjie, Wu Qingbo, etc. Clinical investigation and treatment of canine dermatomycosis [J]. Heilongjiang Journal of Animal Husbandry and Veterinary Medicine, 2015, 000(002):61-64. 5. Shen Fei, Chen Yijie, Xu Xiaolan, etc. Antibacterial mechanism of propolis against Aspergillus fumigatus in vitro [J]. China apiculture, 2016, 25 (4):12-16. |
Overview | Lactated phenol Cotton Blue staining solution, including 10-15 parts PVA, 20-40 parts lactic acid, 20-40 parts phenol and 0.05-0.1 parts aniline blue. Among them, the PVA reagent is used to strengthen the adhesion between the dyeing layer and the glass, and the sealing barrier dyeing layer is oxidized by contact with air. The phenol reagent was used as a fixing agent to enhance the affinity of hyphae, spores and stain, and improve the dyeing effect, and the effect was Disinfection. The lactic acid reagent is used to adjust the PH and increase the solubility. The aniline blue reagent functions as a coloring agent. The lactic acid phenol Cotton Blue staining solution can be used to seal the dyed wet and dry pieces, and the application range is wider; The fungal wet pieces or glass slides can be preserved permanently, and the flat surface is dried for at least 24 hours, these blocked tablets are permanent and do not dissolve in ether, xylene or ethanol. The fixed fungal structure can be clearly maintained for several years, and the preservation effect is better. The Structure of fungal hyphae and spores can be observed under the low magnification, high magnification and oil microscope, the original staining method can only be observed at low times and high times. Lactic acid phenol cotton blue dyeing solution set of dyeing function and can save the function of preparation in one, ordinary dyeing solution only dyeing function. |
preparation | the preparation method of lactated phenol Cotton Blue staining solution includes the following steps: 10 to 15 parts of PVA powder are added to a container containing water, and the container may be a beaker, a test tube, or the like. The vessel is heated, in particular the beaker can be placed on a heating plate. At the same time, the metal ions in the solution are adsorbed by stirring with a magnetic rod. Then add 20-40 parts of lactic acid, 20-40 parts of phenol crystal and 0.05-0.1 parts of aniline blue successively; Continue heating the solution and mix the solution with a magnetic bar until the temperature reaches 70-90 ℃, and stop heating, preparation of lactic acid phenol Cotton Blue staining solution. Therein, a thermometer may be added to the container to detect the temperature. The container is filled with deionized water to prevent metal ions in the water from affecting the dyeing effect of the dyeing solution. After the heating is stopped, the prepared lactic acid phenol Cotton Blue staining solution may be cooled, then sub-packed into a drop bottle, closed the bottle cap, and stored at room temperature. |
Use | Aspergillus fumigatus and other staining |