106070-31-9

106070-31-9 - Names and Identifiers

Name	2',7'-dichlorodihydrofluorescein
Synonyms	2',7'-Dichlorofluorescin Dichlorodihydrofluorescein 2',7'-dichlorodihydrofluorescein 2-(2,7-Dichloro-3,6-dihydroxy-9H-xanthen-9-yl)benzoic acid Benzoic acid, 2-(2,7-dichloro-3,6-dihydroxy-9H-xanthen-9-yl)-
CAS	106070-31-9

106070-31-9 - Physico-chemical Properties

Molecular Formula	C20H12Cl2O5
Molar Mass	403.21
Storage Condition	2-8℃
Physical and Chemical Properties	EPA Chemical Information Benzoic acid, 2-(2,7-dichloro-3,6-dihydroxy-9H-xanthen-9-yl)- (106070-31-9)

106070-31-9 - Upstream Downstream Industry

Raw Materials	Benzoic acid, 2-(2,7-dichloro-6-hydroxy-3-oxo-3H-xanthen-9-yl)- 2′,7′-Dichlorodihydrofluorescein Diacetate
Downstream Products	2',7'-DICHLOROFLUORESCEIN AFMK

106070-31-9 - Introduction

2 ',7'-dichlorodihydrofluorescein, also known as DCFH-DA, is a fluorescent probe. It is a colorless crystal that can be dissolved in organic solvents, but insoluble in water.

2 ',7'-dichlorodihydrofluorescein can be converted into strong green fluorescence in cells, which can be used to detect many biologically active molecules and cellular responses by measuring the fluorescence intensity. It is mainly used to measure the level of intracellular oxidative stress, because it can react with reactive oxygen species to generate fluorescent products. In addition, it is widely used to evaluate biological processes such as apoptosis and cytotoxicity.

Preparation of 2 ',7'-dichlorodihydrofluorescein requires synthesis from brominated phenol and aniline. First, The brominated phenol reacts with aniline under alkaline conditions to obtain the precursor compound of 2 ',7'-dichlorodihydrofluorescein, and then the target product is obtained by reduction reaction. The preparation process should be carried out in an anhydrous organic solvent to avoid interference of moisture on the reaction.

Regarding safety information, 2 ',7'-dichlorodihydrofluorescein is relatively safe under general conditions, but it still needs to be used with caution. It has a certain eye irritation and respiratory tract irritation, and inhalation or contact with eyes should be avoided. When in use, you should wear appropriate personal protective equipment, such as laboratory gloves and safety glasses, and operate in a well-ventilated area. Local regulations and safe practices should be followed when handling and disposing of waste.

Last Update：2024-04-09 21:01:54