2-Nitrophenyl Beta-D-Galactoside-(1,5)
ortho-Nitrophenyl-β-galactoside
CAS: 369-07-3;19710-96-4
Molecular Formula: C12H15NO8
2-Nitrophenyl Beta-D-Galactoside-(1,5) - Names and Identifiers
| Name | ortho-Nitrophenyl-β-galactoside |
| Synonyms | ONPG 2-nitrophenylgalactoside 2-Nitrophenylgalactoside 2-Nitrophenyl-B-D-Galactoside 2-nitrophenyl β-D-galactoside ortho-Nitrophenyl-β-galactoside 2-Nitrophenyl-Beta-D-Galactoside 2-Nitrophenyl beta-D-galactoside Galactopyranoside, 2-nitrophenyl 2-nitrophenyl D-galactopyranoside 2-Nitrophenyl-alpha-D-galactoside 2-Nitrophenyl-B-D-Galactopyranoside 2-Nitrophenyl β-D-galactopyranoside 2-Nitrophenyl-β-D-galactopyranoside ortho-Nitrophenyl-beta-D-galactoside 2-nitrophenyl beta-L-glucopyranoside Ortho-nitrophenyl-beta-D-galactoside 2-Nitrophenyl-Beta-D-galactopyranoside 2-Nitrophenyl Beta-D-Galactoside-(1,5) 2-nitrophenyl beta-D-galactopyranoside 2'-Nitrophenyl-Beta-D-Galactopyranoside 2-nitrophenyl alpha-L-erythro-hexopyranoside |
| CAS | 369-07-3 19710-96-4 |
| EINECS | 206-716-1 |
| InChI | InChI=1/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-4-2-1-3-6(7)13(18)19/h1-4,8-12,14-17H,5H2/t8?,9-,10-,11?,12+/m0/s1 |
2-Nitrophenyl Beta-D-Galactoside-(1,5) - Physico-chemical Properties
| Molecular Formula | C12H15NO8 |
| Molar Mass | 301.25 |
| Density | 1.599±0.06 g/cm3(Predicted) |
| Melting Point | 195℃ |
| Boling Point | 572.4±50.0 °C(Predicted) |
| Flash Point | 300°C |
| Vapor Presure | 6.1E-14mmHg at 25°C |
| pKa | 12.69±0.70(Predicted) |
| Refractive Index | 1.647 |
| MDL | MFCD00063255 |
2-Nitrophenyl Beta-D-Galactoside-(1,5) - Risk and Safety
| Safety Description | S24/25 - Avoid contact with skin and eyes. |
2-Nitrophenyl Beta-D-Galactoside-(1,5) - Reference
| Reference Show more | 1. Zhou Yu, Li, Xingxin, Yifan, et al. Optimization of fermentation conditions for β-galactosidase production by Lactobacillus acidophilus [J]. Food Science, 2011, 32(23):180-185. 2. Zhang Qingqing, Li Huijing, Cao Gansheng, et al. Effects of astragalus on growth and enzyme production of Lactobacillus acidophilus [J]. Science and Technology of food industry, 2013(15):166-169. 3. Qian Weidong, Fu Yunfang, Hu Na. Study on fermentation conditions of lactase production by Klyveromyces lactis yeast [J]. Chinese agricultural bulletin, 2012. Note: For some products, our company can only provide some information, we do not guarantee the authority of the information provided, only for the reference of the customer. use: for the detection of β-galactosidase (β-galactosidase) activity storage conditions: -20 ℃ sensitivity: easy to absorb moisture solubility: soluble in water, methanol Appearance: White or almost white crystalline powder specific optical rotation: -68 · 8 ° (C = 1, H2O) Melting Point: 195 ° C. Is used to detect β-galactosidase (β-galactosidase) activity and is a chromogenic substrate for β-galactosidase. After being catalyzed by β-galactosidase, a yellow product can be produced, and the absorbance can be detected at the wavelength of 410-420nm |
2-Nitrophenyl Beta-D-Galactoside-(1,5) - Introduction
Galactosidase, also known as fluorite (ONPG), is a chemical reagent commonly used as a substrate for the detection of β-galactosidase activity.
The chemical formula of ONPG is C14H15NO8, which is a white to light yellow crystal. It is soluble in water but insoluble in organic solvents such as ethanol and ether. Its melting point is 145-149°C.
ONPG is widely used in biochemistry and molecular biology. It can be hydrolyzed by β-galactosidase to produce a yellow product and thus be used to detect the activity of β-galactosidase or as a substrate for β-galactosidase. This reagent is commonly used in synthetic assays, gene expression, and yeast action experiments.
The method for preparing ONPG is relatively simple, usually by reacting ortho-nitrophenyl thiomannan with β-D-galactosase to generate ONPG.
For safety information, ONPG is a relatively safe chemical reagent, but it still needs to be used with caution. Laboratory safety guidelines should be followed during the operation, and attention should be paid to protective measures, such as wearing appropriate protective clothing, gloves and glasses. Avoid inhalation, contact with skin and eyes. In case of contact with skin or eyes, rinse immediately with plenty of water and seek medical help. At the same time, the chemical agent should be protected from contact with oxidizing agents or flammable materials to avoid danger.
The chemical formula of ONPG is C14H15NO8, which is a white to light yellow crystal. It is soluble in water but insoluble in organic solvents such as ethanol and ether. Its melting point is 145-149°C.
ONPG is widely used in biochemistry and molecular biology. It can be hydrolyzed by β-galactosidase to produce a yellow product and thus be used to detect the activity of β-galactosidase or as a substrate for β-galactosidase. This reagent is commonly used in synthetic assays, gene expression, and yeast action experiments.
The method for preparing ONPG is relatively simple, usually by reacting ortho-nitrophenyl thiomannan with β-D-galactosase to generate ONPG.
For safety information, ONPG is a relatively safe chemical reagent, but it still needs to be used with caution. Laboratory safety guidelines should be followed during the operation, and attention should be paid to protective measures, such as wearing appropriate protective clothing, gloves and glasses. Avoid inhalation, contact with skin and eyes. In case of contact with skin or eyes, rinse immediately with plenty of water and seek medical help. At the same time, the chemical agent should be protected from contact with oxidizing agents or flammable materials to avoid danger.
Last Update：2024-04-10 22:29:15
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Product Name: ONPG Visit Supplier Webpage Request for quotationCAS: 369-07-3
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Wechat: 18301782025
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