3,6-bis(diMethylaMino)-9-(2-(Methoxycarbonyl)phenyl)xanthyliuM perchlorate

Name	TMRM [Tetramethylrhodamine, methyl ester, perchlorate]
Synonyms	TMRM T 668 TMRM, PERCHLORATE Tetramethylrhodamin methylester perchlorate TETRAMETHYLRHODAMINE METHYL ESTER PERCHLORATE TMRM [Tetramethylrhodamine, methyl ester, perchlorate] 3,6-bis(diMethylaMino)-9-(2-(Methoxycarbonyl)phenyl)xanthyliuM perchlorate
CAS	115532-50-8

Molecular Formula	C25H25ClN2O7
Molar Mass	500.93
Melting Point	274–276℃
Solubility	DMSO: soluble
Appearance	crystals
Color	Dark green
Maximum wavelength(λmax)	549 nm
BRN	7162165
Storage Condition	Sealed in dry,Room Temperature
MDL	MFCD00274460
Use	Potential-Sensitive fluorescent probe for measuring membrane potential changes in mitochondria
In vitro study	TMRM Perchlorate is a fluorescent probe (excitation, 530±21 nm; emission, 592±22 nm). The fluorescence signal in the presence of TMRM Perchlorate shows a slight decrease after the addition of glutamate, indicative of increased polarization of the mitochondrial inner membrane. In the presence of TMRM Perchlorate (2 μM) the coupled respiration with Complex I substrates or upon the addition of Complex II substrate is decreased by 27%. Exposure of hippocampal cultures to low concentrations of TMRM Perchlorate (50 to 500 nM) for 1 to 3 hours results in selective staining of mitochondria in both neurons and the underlying glial cells. Exposure of hippocampal cultures to high concentrations of TMRM Perchlorate (1 to 25 µM) stains mitochondria selectively and quickly, reaching a plateau after 5 to 10 min. Low concentrations of TMRM Perchlorate (50 to 200 nM) do not induce apoptosis, whereas higher concentrations (0.5 and 2.5 µM) enhance apoptosis (K D = 500 nM).

Hazard Symbols	Xi - Irritant
Risk Codes	36/37/38 - Irritating to eyes, respiratory system and skin.
Safety Description	S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36 - Wear suitable protective clothing.
WGK Germany	3
FLUKA BRAND F CODES	8-10

Biological activity	TMRM Perchlorate is a cell-permeable cationic lipophilic red fluorescent dye (λex = 530 nm, λem = 592 nm).
in vitro study	TMRM Perchlorate is a fluorescent probe (excitation, 530±21 nm; emission, 592±22 nm). The fluorescence signal in the presence of TMRM Perchlorate shows a slight decrease after the addition of glutamate, indicative of increased polarization of the mitochondrial inner membrane. In the presence of TMRM Perchlorate (2 μ M) the coupled respiration with Complex I substrates or upon the addition of Complex II substrate is decreased by 27%. Exposure of hippocampal cultures to low concentrations of TMRM Perchlorate (50 to 500 nm) for 1 to 3 hours results in selective staining of mitochondria in both neurons and the underlying glial cells. Exposure of hippocampal cultures to high concentrations of TMRM Perchlorate (1 to 25 m) stains mitochondria selectively and quickly, reaching a plateau after 5 to 10 min. Low concentrations of TMRM Perchlorate (50 to 200 nM) do not induce apoptosis, whereas higher concentrations (0.5 and 2.5 m) enhance apoptosis (K D = 500 nM).
biological field application	Detecting mitochondrial membrane potential; apoptosis assays; multidrug resistance assays

Last Update：2024-04-10 22:29:15

Product Name: TMRM [Tetramethylrhodamine, methyl ester, perchlorate] Visit Supplier Webpage Request for quotation
CAS: 115532-50-8
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409

Wechat: 18301782025

Wechat: 18301782025

View History

3,6-bis(diMethylaMino)-9-(2-(Methoxycarbonyl)phenyl)xanthyliuM perchlorate Request for Quotation