Molecular Formula | C12H8ClNO4S |
Molar Mass | 297.71 |
Melting Point | 115 °C |
Solubility | Soluble in DMSO (30 mg/ml) |
Appearance | solid |
Color | White to off-white |
Storage Condition | Sealed in dry,Room Temperature |
Stability | Stable for 2 years from date of purchase as supplied. Solutions in DMSO may be stored at -20°C for up to 2 months. |
In vitro study | BTB-1 blocks the motility of Kif18A in a reversible manner. BTB-1 inhibits Kif18A in an adenosine triphosphate (ATP)-competitive but microtubule-uncompetitive manner and slows down the progression of cells through mitosis. 100 μM BTB-1 does not significantly inhibit any of the other tested mitotic kinesins. BTB-1 competes with ATP for Kif18A binding only when the motor-protein is associated with its pseudosubstrate microtubules. HeLa cells treated with BTB-1 accumulate in mitosis in a dose-dependent manner. BTB-1 shows cell toxicity with an EC 50 values of 35.8 μM. HeLa cells treated with 50 μM BTB-1 reveals severe defects in spindle morphology and chromosome alignment. Treatment with high concentrations of BTB-1 does not result in elongated spindles. |
Risk Codes | 36/37/38 - Irritating to eyes, respiratory system and skin. |
Safety Description | S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36/37/39 - Wear suitable protective clothing, gloves and eye/face protection. |
Hazard Class | IRRITANT |
1mg | 5mg | 10mg | |
---|---|---|---|
1 mM | 3.359 ml | 16.795 ml | 33.59 ml |
5 mM | 0.672 ml | 3.359 ml | 6.718 ml |
10 mM | 0.336 ml | 1.679 ml | 3.359 ml |
5 mM | 0.067 ml | 0.336 ml | 0.672 ml |
biological activity | BTB-1 is a novel, reversible and selective inhibitor of mitotic motor protein Kif18A, the IC50 value was 1.69 μm. |
Target | Value |
Kif18A (Cell-free assay) | 1.69 μM |