5-FAM Maleimide
5-FAM Maleimide
CAS: 787632-00-2
Molecular Formula: C27H18N2O8
5-FAM Maleimide - Names and Identifiers
| Name | 5-FAM Maleimide |
| Synonyms | 5-FAM-Mal 5-FAM MALEIMIDE 5-FAM Maleimide N-[2-(2,5-dioxopyrrol-1-yl)ethyl]-3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxamide(5-FAM Maleimide) |
| CAS | 787632-00-2 |
5-FAM Maleimide - Physico-chemical Properties
| Molecular Formula | C27H18N2O8 |
| Molar Mass | 498.44 |
| Appearance | Yellow Crystalline Powder |
5-FAM Maleimide - Introduction
5-FAM Maleimide(5-FAM Maleimide) is a fluorescent probe molecule commonly used in biomarker labeling and detection assays. The following is a description of its nature, use, preparation and safety information:
Nature:
-chemical formula: C19H10N2O5S
-Molecular weight: 382.36g/mol
-Appearance: yellow powder
-Solubility: Soluble in organic solvents such as DMF, DMSO and methanol
- pH stability: suitable for neutral or weak alkaline environment (pH 7-8)
Use:
-Biomarkers: Can react with amino acids containing sulfhydryl groups (-SH) in oligopeptides, proteins and other biomolecules to give them observable fluorescence properties, for cell imaging and protein localization.
-Detection and analysis: It can be used to detect and determine biological molecules containing thiol groups, which is of great significance for the study of molecular interactions and reactions.
Method:
-The commonly used preparation method is to react 5-FAM Maleimide with a compound containing a thiol group to produce a compound similar to an amide structure. The reaction is usually carried out in an organic solvent, and the selectivity and yield of the reaction can be controlled by adjusting the ratio of the reactants and the reaction conditions.
Safety Information:
- 5-FAM Maleimide needs to be careful to prevent skin contact and inhalation during operation, because it may be toxic to the human body. When using and handling, please wear gloves and protective glasses, and make sure to operate in a well-ventilated environment. In addition, waste and solutions should be disposed of in the correct way. If skin contact or inhalation occurs, rinse immediately with plenty of water and seek medical help.
Nature:
-chemical formula: C19H10N2O5S
-Molecular weight: 382.36g/mol
-Appearance: yellow powder
-Solubility: Soluble in organic solvents such as DMF, DMSO and methanol
- pH stability: suitable for neutral or weak alkaline environment (pH 7-8)
Use:
-Biomarkers: Can react with amino acids containing sulfhydryl groups (-SH) in oligopeptides, proteins and other biomolecules to give them observable fluorescence properties, for cell imaging and protein localization.
-Detection and analysis: It can be used to detect and determine biological molecules containing thiol groups, which is of great significance for the study of molecular interactions and reactions.
Method:
-The commonly used preparation method is to react 5-FAM Maleimide with a compound containing a thiol group to produce a compound similar to an amide structure. The reaction is usually carried out in an organic solvent, and the selectivity and yield of the reaction can be controlled by adjusting the ratio of the reactants and the reaction conditions.
Safety Information:
- 5-FAM Maleimide needs to be careful to prevent skin contact and inhalation during operation, because it may be toxic to the human body. When using and handling, please wear gloves and protective glasses, and make sure to operate in a well-ventilated environment. In addition, waste and solutions should be disposed of in the correct way. If skin contact or inhalation occurs, rinse immediately with plenty of water and seek medical help.
Last Update：2024-04-10 22:29:15
