Name | RHODAMINES |
Synonyms | RHODAMINES |
CAS | 67226-84-0 |
Molecular Formula | C22H27ClN2O3 |
Molar Mass | 402.91438 |
Rhodamines are a group of fluorescent dyes that are commonly used in various applications such as fluorescent microscopy, flow cytometry, and DNA sequencing. They are typically synthesized by derivatizing fluorescein with an amine or other reactive group. Rhodamine dyes are known for their bright red-orange fluorescence and high photostability, making them useful for a wide range of scientific and industrial purposes.
Properties:
- Rhodamine dyes typically exhibit bright orange-red fluorescence with high intensity and stability.
- They can show fluorescence peaks at excitation and emission wavelengths in solution.
- The molecular structure of Rhodamine dyes often includes amino groups, allowing them to bind to biomolecules or amino acids in cells for labeling and tracking purposes.
Uses:
- In the field of life sciences, Rhodamine dyes are commonly used for cell fluorescence labeling, protein and nucleic acid staining, cell tracking, etc.
- In medical diagnostics, Rhodamine dyes can be used for pathological testing and analysis.
- They play a role in fluorescence labeling in instruments such as fluorescence microscopes, flow cytometers, enzyme immunoassays, etc.
Synthesis:
- The synthesis of Rhodamine dyes typically involves introducing different functional groups, such as amino groups or others, onto the fluorescein structure to alter the fluorescence properties or enhance binding characteristics.
- Specific synthetic methods may involve multi-step reactions and specific chemical reaction conditions depending on the desired final product.
Safety:
- Rhodamine dyes are generally considered relatively safe but should still be handled following laboratory safety procedures.
- Direct skin contact may cause discomfort or allergic reactions, so proper protective gear should be worn when working with them.
- Avoid inhalation or ingestion of Rhodamine dye solutions and perform operations in a well-ventilated laboratory setting.