Molecular Formula | C17H20ClN3 |
Molar Mass | 301.81 |
Density | 1.001g/mLat 20°C |
Melting Point | 284-287°C(lit.) |
Solubility | Soluble in water, ethanol, dimethyl sulfoxide |
Appearance | Powder |
Color | orange to red |
Maximum wavelength(λmax) | ['500 nm'] |
BRN | 3734978 |
pKa | -3.2, 10.5(at 25℃) |
Storage Condition | -20°C |
Refractive Index | n20/D 1.338 |
In vitro study | Acridine Orange has been employed extensively as a cytochemical stain and has shown to stain differentially DNA and RNA, and double-restranded nucleic acids in situ . Acridine Orange can either intercalate into double helical nucleic acids (green fluorescence at 530 nm), or bind electrostatically to phosphate groups of single-stranded molecules (red fluorescence at 640 nm). This unique characteristic makes acridine orange useful for cell-cycle studies. Acridine Orange staining of unfixed cells may be used as a simple, fast means of obtaining information on cell ploidy levels and cell cycle status from DNA measurements (green fluorescence), and cell transcriptional activity from RNA staining (red fluorescence), in human and murine cells lines, peripheral blood and bone marrow specimens from patients with leukemia and mitogenically (phytohemagglutinin) or antigenically (mixed lymphocyte culture) stimulated human peripheral blood cultures. |
Safety Description | S22 - Do not breathe dust. S24/25 - Avoid contact with skin and eyes. |
WGK Germany | 3 |
RTECS | AR7601000 |
HS Code | 32041300 |
Toxicity | dnd-mus:ast 20 mmol/L BBACAQ 374,96,74 |
Color index | 46005 |
Main application | adhetions; Aluminum crystal crystal materials; detecting clay particles; display device; evaluating fiber surface characteristics; glass rixes; imaging material; inks; lasers; recording materials; photoresists; Textures; thin films; tracers for chemistry; Withing boards |
Biological applications | Detecting cancer cells,spores,human papilloma virus (HPV),stress biomarkers; |
EPA chemical substance information | information provided by: ofmpeb.epa.gov (external link) |
biological activity | Acridine Orange hydrate is a cell-permeable fluorescent dye that binds to nucleic acids, resulting in altered emission spectra. |
category | toxic substances |
flammability hazard characteristics | thermal decomposition of toxic chlorides, hydrogen chloride and NOx fumes |
storage and transportation characteristics | ventilation and low temperature drying |
fire extinguishing agent | dry powder, foam, sand, carbon dioxide, water mist |