Buffer Solution pH3.6 (C8H5KO4-HCl) - Names and Identifiers
Buffer Solution pH3.6 (C8H5KO4-HCl) - Physico-chemical Properties
Storage Condition | Room Temprature |
Use | This product is for scientific research only and shall not be used for other purposes. |
Buffer Solution pH3.6 (C8H5KO4-HCl) - Reference
Reference Show more | 1. Cheng Guangyu, Cong Tingting, Lu Meixiang, Zheng Feifei, He Ze. Effects of detoxification, dredging collaterals and protecting kidney method on PI3K/Akt signaling pathway activation-related factors ILK, Akt and podocyte apoptosis rate in mouse podocytes stimulated by high glucose [J]. Chinese Journal of Gerontology, 2019,39(05):1174-1176. 2. Yang Jun, Cheng Huilei, Wei Dafu, etc. Preparation and antibacterial activity of poly (hexamethylene guanidine)-bonded polypropylene wax [J]. Journal of Functional Polymers, 2012(04):43-47. 3. Peng Jinli, Yu Juan, Duan Xiaopeng, Yin Tao. Preparation, cellular uptake and induction of apoptosis in breast cancer PEG-PE cells of cryptotanshinone MDA-MB-231 nano-micelles [J]. Central South pharmacy, 2019,17(06):842-847. 4. Feng Lan, an Tianwu, Cui Pengfei, Zhao Xiaodong, Li Huade, Fu Xue, Zhang Lan, Zha Ya Ru, Li Hao, Yan Wenjun, Wang Hongning, Yang Xin. Study on the isolation, identification and pathogenicity of salmonella delvia in yak [J]. Journal of Sichuan University (Natural Science Edition),2020,57(05):1022-1028. 5. He, R., et al. "Incorporated 131 I into a PAMAM (g5. 0) dendrimer-conjugate: design of a thermal nanosensor for medullary thyroid carcinoma." Rsc Advances 7.26 (2017): 16181-16188.https:// doi.org/10.1039/C7RA00604G |
Buffer Solution pH3.6 (C8H5KO4-HCl) - Introduction
PBS buffer is a commonly used biochemical buffer that is widely used in research and laboratory applications. The following are the properties, uses, preparation and safety information of PBS buffer (50mM, pH 3.6):
Nature:
-Composition: Mainly contains phosphate and chloride plasma. Common formulations are NaCl, KCl, Na2HPO4, KH2PO4, etc.
- pH: Under this specific formula, the pH value is about 3.6, which is suitable for certain specific experimental conditions and applications.
-Stability: PBS buffer is relatively stable to some common chemical and temperature changes.
Use:
-Cell Culture: PBS buffer is commonly used in washing and handling steps during cell culture.
-Biomolecule Research: PBS buffer can be used as a solubilization and dilution solution for biomolecules such as proteins, DNA and RNA.
-Immunostaining: In immunohistochemistry and immunohistochemistry experiments, PBS buffer is used to wash and dilute reagents such as antibodies.
-Biochemical experiment: In many biochemical experiments, PBS buffer is used as dilution reagent, washing liquid, stable sample pH value, etc.
Preparation Method:
-Core Formulation: The core formulation of PBS buffer includes 75 mM NaCl, 2 mM KCl, 8 mM Na2HPO4, and 2 mM KH2PO4. The pH and concentration can be adjusted as needed.
-Preparation method: Prepare the above ingredients at specific concentrations, dissolve them in deionized water, and adjust the pH to prepare 50 mM, pH 3.6 PBS buffer.
Safety Information:
- PBS buffer is generally non-flammable, but you still need to pay attention to avoid fire and high temperature.
-When using, avoid contact with sensitive parts such as skin, eyes and mouth. In case of contact, flush with plenty of water and seek medical help if necessary.
-in the operation and storage of PBS buffer, to avoid contact with organic matter, acid, alkali and other substances, so as to avoid unnecessary reaction.
Last Update:2024-04-09 15:18:08