C.I. Direct Blue 14, tetrasodium salt
Trypan Blue
CAS: 72-57-1
Molecular Formula: C34H24N6Na4O14S4
C.I. Direct Blue 14, tetrasodium salt - Names and Identifiers
| Name | Trypan Blue |
| Synonyms | C.I. 23850 Trypan Blue azidinblau3b azidineblue3b amidineblue4b amanilskybluer Direct Blue 14 Niagara Blue 3B azurrodiretto3b C.I. Direct Blue 14 Trypan Blue solution Direct Blue 14 (C.I.) C.I. Direct Blue 14, tetrasodium salt C.I. Direct Blue 14, tetrasodium salt (8CI) 2,7-naphthalenedisulfonicacid,3,3'-[(3,3'-dimethyl[1,1'-biphenyl]-4,4'- 2,7-naphthalenedisulfonicacid,3,3'-((3,3'-dimethyl-4,4'-biphenylylene)bis(azo tetrasodium 3,3'-[(3,3'-dimethylbiphenyl-4,4'-diyl)di(E)diazene-2,1-diyl]bis(5-amino-4-hydroxynaphthalene-2,7-disulfonate) 2,7-Naphthalenedisulfonicacid,3,3'-[[3,3'-dimethyl(1,1'-biphenyl)-4,4'-diyl]bis(azo)]bis(5-amino-4-hydroxy-,tetrasodiumsalt tetrasodium (3Z,3'Z)-3,3'-[(3,3'-dimethylbiphenyl-4,4'-diyl)di(1Z)hydrazin-2-yl-1-ylidene]bis(5-amino-4-oxo-3,4-dihydronaphthalene-2,7-disulfonate) |
| CAS | 72-57-1 |
| EINECS | 200-786-7 |
| InChI | InChI=1/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,37-38H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4/b39-31+,40-32+;;; |
C.I. Direct Blue 14, tetrasodium salt - Physico-chemical Properties
| Molecular Formula | C34H24N6Na4O14S4 |
| Molar Mass | 960.81 |
| Density | 1.007g/mLat 20°C |
| Melting Point | >300 °C (lit.) |
| Water Solubility | 10 g/L (25 ºC) |
| Solubility | H2O: soluble10mg/mL |
| Appearance | Powder |
| Color | Dark greenish-brown |
| Merck | 14,9792 |
| BRN | 4360496 |
| PH | 9.8 (10g/l, H2O, 20℃) |
| Storage Condition | dry at room temperature |
| Stability | Stable. Incompatible with strong oxidizing agents. |
| MDL | MFCD00003969 |
| Physical and Chemical Properties | Blue-gray powder, soluble in water is blue, slightly soluble in soluble fiber yarn, insoluble in other organic solvents. Dark green blue in concentrated sulfuric acid, Red Blue after dilution; Brown gray solution in concentrated nitric acid. Its aqueous solution, in case of 10% sulfuric acid is red; In case of sodium hydroxide is red purple. |
C.I. Direct Blue 14, tetrasodium salt - Risk and Safety
| Risk Codes | R45 - May cause cancer R41 - Risk of serious damage to eyes R37/38 - Irritating to respiratory system and skin. R36/37/38 - Irritating to eyes, respiratory system and skin. R22 - Harmful if swallowed |
| Safety Description | S53 - Avoid exposure - obtain special instructions before use. S45 - In case of accident or if you feel unwell, seek medical advice immediately (show the label whenever possible.) S36/37/39 - Wear suitable protective clothing, gloves and eye/face protection. S36 - Wear suitable protective clothing. S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. |
| UN IDs | 2811 |
| WGK Germany | 3 |
| RTECS | QJ6475000 |
| TSCA | Yes |
| HS Code | 32041400 |
| Toxicity | LD100 i.v. in rats: 300 mg/kg (Anderson) |
C.I. Direct Blue 14, tetrasodium salt - Reference
| Reference Show more | 1. Wang Huafu, Gui Zhihong, Zhang Xiaoru, etc. Pharmacokinetics of Liuwei Dihuang pill in diabetic rats with kidney yin deficiency by microdialysis [J]. Northwest Pharmaceutical Journal, 2019, 034(001):87-90. 2. Chen Lie, Sun Sheng, Yu Changxia, etc. Isolation of lactic acid bacteria from traditional foods in northwest China and screening of immune enhancing strains [J]. China Dairy Industry, 2019(10). 3. Li Chengzhong, Zhang Huanxin, Li ruomin, Sun Yan, Feng Liguo. Study on compound preparation of peony seed oil and its hypoglycemic and immune activity enhancement [J]. Journal of Yangzhou University (Agriculture and Life Science Edition),2020,41(06):84-89 4. Yao, Wendong, et al. "Flexible two-layer discarding and safing microneedle transdermal of neurotoxin: a bioformable attempt to treat Rheumatoid Arthritis." International journal of pharmaceutics 563 (2019): 91-100.https:// doi.org/10.1016/j. Ijpharm.2019. 5. [IF = 5.875] Wendong Yao et al."Flexible two-layer dissolving and safing microneedle transdermal of neurotoxin: A biocomfortable attempt to treat Rheumatoid Arthritis."Int J Pharmaceut. 2019 May;563:91 |
C.I. Direct Blue 14, tetrasodium salt - Introduction
Blue-gray powder, soluble in water and blue, slightly soluble in fibrillated yarn, insoluble in other organic solvents. In case of concentrated sulfuric acid, it is dark green and blue, and it is red and blue after dilution; in case of concentrated nitric acid, it is a brown and gray solution. Its aqueous solution is red when it meets 10% sulfuric acid; when it meets sodium hydroxide, it is red and purple.
Last Update：2022-10-16 17:28:09
C.I. Direct Blue 14, tetrasodium salt - Reference Information
| Color index | 23850 |
| (IARC) carcinogen classification | 2B (Vol. 8, Sup 7) 1987 |
| EPA chemical substance information | information provided by: ofmpeb.epa.gov (external link) |
| Introduction | 3,3 '-{[3,3'-dimethyl-(1,1 '-diphenyl)-4,4'-diyl] bis (AZO)}-bis (5-amino-4-hydroxy-2, 7-naphthalene disulfonic acid) tetrasodium salt also called trypan blue, it is a cell-reactive dye that is commonly used to detect the integrity of cell membranes. Normal living cells, with intact cell membrane structure, can reject 3,3 '-{[3,3'-dimethyl-(1,1 '-diphenyl)-4,4'-diyl] bis (AZO)}-bis (5-amino-4-hydroxy-2, 7-naphthalene disulfonic acid) tetrasodium salt, making it unable to enter the cell; while the loss of activity or cell membrane is incomplete, the permeability of the cell membrane can be increased by 3,3 '-{[3,3'-dimethyl-(1,1 '-diphenyl)-4,4'-diyl] bis (AZO)}-bis (5-amino-4-hydroxy-2, 7-naphthalene disulfonic acid) tetrasodium salt was stained blue. It is generally considered that the cell membrane integrity is lost, that is, the cell has died. |
| Application example | with the help of 3,3 '-{[3,3'-dimethyl-(1,1 '-diphenyl)-4,4'-diyl] bis (AZO)}-bis (5-amino-4-hydroxy-2, 7-naphthalene disulfonic acid) tetrasodium salt dyeing can be very simple, rapid discrimination between live and dead cells. 3,3 '-{[3,3'-dimethyl-(1,1 '-diphenyl)-4,4'-diyl] bis (AZO)}-Bis (5-amino-4-hydroxy-2, 7-naphthalene disulfonic acid) tetrasodium salt is one of the most commonly used staining methods for the identification of dead cells in tissue and cell culture. Note that apoptotic bodies also have 3,3 '-{[3,3'-dimethyl-(1,1 '-diphenyl)-4,4'-diyl] bis (AZO)}-Bis (5-amino-4-hydroxy-2, 7-naphthalene disulfonic acid) tetrasodium salt as a dye-repellent phenomenon. |
| Use | mainly used for cotton, hemp, viscose and other cellulose fiber dyeing, can also be used for silk, nylon, the dyeing of sticky/cotton mixed imitation spinning can also be used for paper, leather and biological dyeing. cell stain |
| production method | after diazotization of 3,3 '-dimethylbenzidine with sodium nitrite and hydrochloric acid, coupling with H acid in alkaline medium, followed by salting out, filtration and drying. Kg/T 3,3 '-dimethylbenzidine (100%) 100H acid (100%) 345 sodium nitrite (100%) 72 soda ash 380 hydrochloric acid (31%) 490 refined salt, 2700 Yuan powder 33 |
| toxic substance data | information provided by: pubchem.ncbi.nlm.nih.gov (external link) |
Last Update：2024-04-09 21:50:46
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C.I. Direct Blue 14, tetrasodium salt
25mm灭菌针式滤器尼龙膜(有机系)0.45um
肌醇半乳糖苷
FMOC-ATC
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25mm灭菌针式滤器尼龙膜(有机系)0.45um
肌醇半乳糖苷
FMOC-ATC
齐墩果酸3-O-beta-D-葡吡喃糖基(1→2)-alpha-L-吡喃阿拉伯糖苷