C.I. Direct Blue 53, tetrasodium salt (8CI)
Evans Blue
CAS: 314-13-6
Molecular Formula: C34H29N6NaO14S4
C.I. Direct Blue 53, tetrasodium salt (8CI) - Names and Identifiers
| Name | Evans Blue |
| Synonyms | blue Evan DiazoBlue Evans blue Evans Blue Evens Blue C.I. 23860 Direct Blue 53 evans blue fluka C.I. Direct Blue 53 evans blue (C.I. 23860) EVANS BLUE C.I. No 23860 C.I. direct blue 53 tetrasodium salt C.I. Direct Blue 53, tetrasodium salt (8CI) 4,4'-Bis[7-(1-amino-8-hydroxy-2,4-disulfo)naphthylazo]-3,3'-bitolyl tetrasodium salt tetrasodium 6,6-((3,3-dimethyl-(1,1-biphenyl-4,4diyl)bis(azo)bis(4-amino-5-hydroxy-1,3-naphthalenedisulphonate) 6,6'-[(3,3'-Dimethyl[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis(4-amino-5-hydroxy-1,3-naphthalenedisulfonic acid disodium) 1,3-Naphthalenedisulfonic acid, 6,6-(3,3-dimethyl1,1-biphenyl-4,4-diyl)bis(azo)bis4-amino-5-hydroxy-, tetrasodium salt 6,6'-[(3,3'-Dimethyl-4,4'-biphenylylene)bis(azo)]bis[4-amino-5-hydroxy-1,3-naphthalenedisulfonic acid] tetrasodium salt tetrasodium 6,6'-[(3,3'-dimethylbiphenyl-4,4'-diyl)di(E)diazene-2,1-diyl]bis(4-amino-5-hydroxynaphthalene-1,3-disulfonate) 3-[[4'-[(4-Amino-2-methyl-5-methoxyphenyl)azo]-3,3'-dimethoxy-1,1'-biphenyl-4-yl]azo]-4-hydroxynaphthalene-1,5-disulfonic acid disodium salt tetrasodium (6E,6'E)-6,6'-[(3,3'-dimethylbiphenyl-4,4'-diyl)di(1E)hydrazin-2-yl-1-ylidene]bis(4-amino-5-oxo-5,6-dihydronaphthalene-1,3-disulfonate) tetrasodium (6Z)-4-amino-6-({4'-[(2E)-2-(8-amino-1-oxo-5,7-disulfonatonaphthalen-2(1H)-ylidene)hydrazino]-3,3'-dimethylbiphenyl-4-yl}hydrazono)-5-oxo-5,6-dihydronaphthalene-1,3-disulfonate |
| CAS | 314-13-6 |
| EINECS | 206-242-5 |
| InChI | InChI=1/C34H28N6O14S4.4Na/c1-15-11-17(3-7-21(15)37-39-23-9-5-19-25(55(43,44)45)13-27(57(49,50)51)31(35)29(19)33(23)41)18-4-8-22(16(2)12-18)38-40-24-10-6-20-26(56(46,47)48)14-28(58(52,53)54)32(36)30(20)34(24)42;;;;/h3-14,37-38H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4/b39-23+,40-24+;;; |
C.I. Direct Blue 53, tetrasodium salt (8CI) - Physico-chemical Properties
| Molecular Formula | C34H29N6NaO14S4 |
| Molar Mass | 896.86 |
| Water Solubility | 280 g/L (20 ºC) |
| Solubility | DMSO (Slightly), Water (Slightly) |
| Appearance | Blue crystal |
| Color | Dark Brown |
| Merck | 14,3905 |
| Storage Condition | Store at RT. |
| Stability | Stable. Incompatible with strong reducing agents, strong oxidizing agents. |
| MDL | MFCD00004021 |
| Physical and Chemical Properties | Blue crystals with green metallic luster. Very soluble in water, slightly soluble in ethanol, almost insoluble in benzene, chloroform, ether, soluble in acid alkali. Hygroscopicity. Color change at pH near 10. |
| Use | This product is a diagnostic drug, used to determine the amount of blood. |
| In vitro study | Evans Blue inhibits NF-κB DNA binding at low concentrations, such as 100 μm. Evans Blue is a reliable stain for microscopic identification of cell death. Evans Blue is also a blocker of a-amino-3-hydroxy-5-methyl-isoxazole/kainate receptor binding to GluR1,2. |
| In vivo study | Pretreatment of Evans blue can inhibit mast cell degranulation, which may be the main mechanism by which Evans blue prevents animals from producing hypersensitivity and systemic inflammation induced by C48/80. Evans blue modulates non-N-methyl-d-aspartate receptors in rat thalamic neurons, inhibits P2X-purine receptors in rat vas deferens, and inhibits glutamate transporters in rat brain synaptic vesicles. |
C.I. Direct Blue 53, tetrasodium salt (8CI) - Risk and Safety
| Risk Codes | R45 - May cause cancer R63 - Possible risk of harm to the unborn child R11 - Highly Flammable R61 - May cause harm to the unborn child R20/21/22 - Harmful by inhalation, in contact with skin and if swallowed. |
| Safety Description | S53 - Avoid exposure - obtain special instructions before use. S45 - In case of accident or if you feel unwell, seek medical advice immediately (show the label whenever possible.) S36/37 - Wear suitable protective clothing and gloves. S16 - Keep away from sources of ignition. S36/37/39 - Wear suitable protective clothing, gloves and eye/face protection. |
| WGK Germany | 3 |
| RTECS | QJ6440000 |
| TSCA | Yes |
| HS Code | 32129000 |
| Toxicity | LD50 intraperitoneal in mouse: 340mg/kg |
C.I. Direct Blue 53, tetrasodium salt (8CI) - Reference
| Reference Show more | 1. Meng Qiuyan, Shao Minmin, Lu Rumei, etc. Experimental study on screening of parasitic anti-inflammatory active fractions from pomelo [J]. Guangxi Journal of Traditional Chinese Medicine, 2017, 040(002):78-80. 2. Hou Xiaotao, Ma Lina, Deng Jiakang, etc. Study on extraction technology and anti-inflammatory activity of total flavonoids from sugarcane leaves [J]. Chinese traditional medicines, 2013, 35(9):2047-2047. 3. Liu Yuelu, Li Feng, Mo Xiaodong, etc. Study on the correlation between the effect of "detoxification and Analgesia" and the quality of toad venom [J]. Liaoning Journal of Traditional Chinese Medicine, 2017(05):1025-1028. 4. Chen Zewen, Wang Xuefang, Li Xiao, Tong Chao, Chen Tong, Wang Xuebing. Anti-inflammatory activity of different polar parts of forsythia suspensa [J]. Heilongjiang Journal of Animal Science and Veterinary Medicine, 2020, 23 (18):124-128. 5. [IF = 11.556] Li Luo et al."Bioengineering CXCR4-overexpressing cell membrane functionalized ROS-responsive nanotherapy for targeting cerebral ischemia-reperfusion injury." Theranostics. 2021; 11(16): 8043-8056 |
C.I. Direct Blue 53, tetrasodium salt (8CI) - Introduction
Solubility in water: 280g/L (20°C), soluble in ethanol, acid and alkali; Almost insoluble in benzene, chloroform and ether. It's hygroscopic. Discoloration at pH close to 10.
Last Update：2022-10-16 17:24:31
C.I. Direct Blue 53, tetrasodium salt (8CI) - Reference Information
| color index | 23860 |
| (IARC) carcinogen classification | 3 (Vol. 8, Sup 7) 1987 |
| EPA chemical information | Information provided by: ofmpub.epa.gov (external link) |
| biological activity | Evans Blue (Direct Blue 53) is an effective inhibitor of glutamate absorption in synaptic vesicles and is also an AMPA/kainate receptor antagonist. |
| Target | Value |
| AMPA/kainate receptor | |
| vesicular glutamate uptake | 87 nM |
| use | this product is a diagnostic drug and is used to measure the amount of hemolysis. |
| Production method | It is obtained by coupling 3,3 '-dimethylbenzidine with 1-amino-8-naphthol -2, 4-disulfonic acid (1824 acid) after diazotization. |
| category | toxic substances |
| toxicity classification | highly toxic |
| acute toxicity | abdominal cavity-mouse LDL0: 200 mg/kg |
| flammability hazard characteristics | combustible; combustion produces toxic nitrogen oxides, sulfur oxides and sodium oxide fumes |
| storage and transportation characteristics | warehouse ventilation and low temperature drying; separate from food raw materials |
| fire extinguishing agent | dry powder, foam, sand, carbon dioxide, mist water |
Last Update：2024-04-09 21:01:54
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