| Name | CALCIUM5'-RIBONUCLEOTIDES |
| Synonyms | CALCIUM5'-RIBONUCLEOTIDES |
| Physical and Chemical Properties | Chemical properties are white to almost white crystals or powder, odorless and have a special taste. It has the effect of multiplying delicate flavor with L-glutamate. Taste threshold 0.0063%. Easy to absorb moisture, moisture absorption can reach 20% ~ 30%. Soluble in water (25g/100ml water, 20 ℃), slightly soluble in acetone, ether and ethanol. |
| Use | Use 5-disodium isatin is a flavor agent that is allowed to be used at home and abroad. It is less used alone and is often used together with monosodium glutamate. China's provisions can be used for all kinds of food, according to the production needs of the appropriate amount of use. |
action
Disodium 5-ribonucleotide is used in the fields of foreign trade export, scientific research and reagent production.
precautions
flavor enhancer (flavor enhancer). my country's "Hygienic Standard for the Use of Food Additives" (GB2760 ― 1996) stipulates that it can be used in various foods in an appropriate amount according to production needs. This product is often combined with sodium glutamate, and its dosage is about 2% ~ 10% of monosodium glutamate. It can be combined with many other ingredients, such as monosodium glutamate 88%, flavor nucleotide 8%, citric acid 4%; the other component is monosodium glutamate 41%, flavor nucleotide 2%, hydrolyzed animal protein 56%, and disodium succinate 1%. If the ratio of sodium inosine and sodium guanosine is 1: 1, the general dosage is as follows: canned soup, 0.02~0.03 g/kg; Canned asparagus, 0.03~0.04 g/kg; Canned crab, 0.01~0.02 g/kg; Canned fish, 0.03~0.06 g/kg; Canned poultry, sausage, ham, 0.06~0.10 g/kg; Sauce 0.10~0.30 g/kg; Seasoning, 0.10~0.15; Seasoned tomato sauce, 0.10~0.20 g/kg; Mayonnaise, 0.12~0.18 g/kg; Snack food, 0.03~0.07 g/kg; Soy sauce, 0.30~0.50 g/kg; Vegetable juice 0.05~0.10 g/kg; Processed cheese, 0.05~0.10 g/kg; Dehydrated soup powder, 1.0~2.0 g/kg; Quick cooking noodle soup powder, 3.0~6.0 g/kg.
identification method
① preparation of sample solution: weigh 1.0g (accurate to 0.0001g) of this product, dissolve it with water and dilute it to 100 mL, and take 10.0 & micro;L, spot samples with Xinhua No.3 filter paper, unfold 30cm in the unfolding solution (saturated ammonium sulfate: isopropanol: water = 79: 2: 19), take out and air dry or dry, ultraviolet absorption spots (IMP or GMP) are drawn in the ultraviolet analyzer, cut into two 10mm × 100mm small test tubes respectively, add 4.0 mL of 0.01 mol/L hydrochloric acid solution each, put the test tubes into an 80 ℃ constant temperature water bath for 30min, and cool them to form the sample solution containing IMP and GMP.
② determination of maximum absorption wavelength: pour the above samples into a Shi Ying cuvette with 1cm optical path respectively. in the wavelength range of 240 ~ 250nm, 0.01 mol/L hydrochloric acid solution is used for blank test, and the maximum absorption peak wavelength is measured by ultraviolet spectrophotometer respectively. Results Treatment: The maximum absorption peak wavelength is IMP within (250±2)nm and GMP within (256±2)nm.
production method
double enzyme method
Nucleic acid is extracted from yeast cultured in pulp waste liquor or by-product yeast in beer and glutamic acid production with dilute alkali liquor (see 5 '-inosinic acid disodium). The 14% nuclease P1 was added to 2% nucleic acid solution and decomposed at 72~75 ℃ and pH = 5.1~5.3 for 24 hours. Then add 10% deaminase solution, react at 50~55 ℃ for 3 hours, adsorb with cationic resin, elute with deionized water, add 95% ethanol at low temperature after film concentration, and precipitate nucleotide solids.
yeast autolysis
fresh brewer's yeast is washed with water at 0~4 ℃ to remove impurities and bitter taste, and yeast slurry containing solid 10% ~ 15% is obtained after coarse filtration. Then adjust Ph = 6~7 with NaOH solution, and autolysis and extraction at 50~60 ℃: 2.5~4h; After boiling and killing the enzyme, cool and stand; Centrifugal separation to remove cell debris, vacuum concentration obtained extract containing solid 45% ~ 60%.