Chromatographic fixative - Names and Identifiers
Chromatographic fixative - Introduction
Chromatographic fixative is a special solution for chromatography, which is used to fix and stabilize the stationary phase on the column.
Chromatographic fixation usually has the following properties:
1. Stability: Chromatographic fixation should have high chemical stability and be able to maintain the stability of the stationary phase to ensure repeated analysis results.
2. Selectivity: the selectivity of Chromatographic fixation depends on the Chromatographic column used and the compounds to be separated. It should have a certain solubility to ensure the migration speed and separation effect of the sample.
3. Gel: The solvents and additives in the Chromatographic fixation usually have certain gel properties to facilitate separation and the formation of a stationary phase.
4. Temperature stability: The Chromatographic fixative should have good stability at room temperature to ensure that problems related to temperature changes will not occur during the analysis process.
The main use of Chromatographic fixation is as a mobile phase in Chromatographic analysis. It is separated by interacting with the compounds in the sample and on the stationary phase. The choice of Chromatographic fixative is very important to optimize the separation effect and increase the analysis speed.
The method of preparing the Chromatographic fixture depends on the purpose of the analysis and the column used. In general, a Chromatographic fixture can be prepared by mixing solvents, additives, and possibly buffers. This process usually requires strict control of the ratio and mixing sequence to ensure that the prepared fixing solution meets the expected requirements.
When using the Chromatographic fixation, you need to pay attention to the following security information:
1. Chromatographic fixative may contain organic solvents and chemicals. Be careful to prevent inhalation of gases or swallowing liquids. Wear gloves and protective glasses if necessary.
2. When dealing with Chromatographic fixation, avoid contact with skin to prevent irritation or allergic reactions.
3. In the process of operation to maintain good ventilation, in order to prevent the accumulation of solvent vapor.
4. According to the provisions of the laboratory, the correct disposal of the use of Chromatographic fixation and waste, to avoid environmental pollution.
Last Update:2024-04-09 19:05:54