Name | D-LUCIFERIN POTASSIUM SALT |
Synonyms | D-LUCIFERIN K SALT D-Luciferenpotassiumsalt D-LUCIFERIN POTASSIUM SALT D-LUCIFERIN, MONOPOTASSIUM SALT BEETLE LUCIFERIN, POTASSIUM SALT D-LUCIFERIN FIREFLY, POTASSIUM SALT D -LUCIFERIN SYNTHETIC POTASSIUM SALT 4,5-DIHYDRO-2-(6-HYDROXY-2-BENZOTHIAZOLYL)-4-THIAZOLECARBOXYLIC ACID POTASSIUM SALT D-(-)-2-[6'-HYDROXY-2'-BENZOTHIAZOLYL]DELTA-THIAZOLINE-4-CARBOXYLIC ACID POTASSIUM SALT potassium (4S)-2-(6-hydroxy-1,3-benzothiazol-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylate |
CAS | 115144-35-9 |
EINECS | 1592732-453-0 |
InChI | InChI=1/C11H8N2O3S2.K/c14-5-1-2-6-8(3-5)18-10(12-6)9-13-7(4-17-9)11(15)16;/h1-3,7,14H,4H2,(H,15,16);/q;+1/p-1/t7-;/m1./s1 |
InChIKey | UMBKGTQQGYPQBE-OGFXRTJISA-M |
Molecular Formula | C11H7KN2O3S2 |
Molar Mass | 318.41 |
Solubility | water: soluble |
Appearance | powder or crystals |
BRN | 5416262 |
Storage Condition | Inert atmosphere,Store in freezer, under -20°C |
MDL | MFCD00044928 |
In vitro study | D-luciferin is the natural substrate of the enzyme luciferase (Luc), that catalyzes the production of the typical yellowgreen light of fireflies.The present review covers the synthesis of D-luciferin and derivatives or analogues that are substrates or inhibitors of the luciferase from the American firefly Photinus pyralis , the enzyme more frequently used in techniques of in vitro and optical imaging. D-Luciferin exhibits a decrease in the measured K m in PC3M-Luc cell lysates with a K m of 34 μM. |
In vivo study | Bioluminescence imaging (BLI) using the firefly luciferase (Fluc) as a reporter gene and D-luciferin as a substrate is currently the most widely employed technique. The total signal intensity is plotted against the time after D-luciferin injection to generate a time-intensity curve. In addition to the peak signal, the signals at fixed time points (5, 10, 15, and 20 min) after D-luciferin injection are determined as alternatives to the peak signal. The signal in a given time-intensity curve is normalized for the peak signal in the curve to represent the pattern of temporal changes after D-luciferin injection. Inject with 10 μL of D-luciferin (intraperitoneally or intravenously) stock solution per gram of body weight: normally ~200 μL for a 20 g mouse for a standard 150 mg/kg injection. Thaw D-Luciferin (either Potassium or Sodium Salt) at room temperature and dissolve in dPBS (no calcium or magnesium) to a final concentration of 15 mg/mL. Pre-wet a 0.22 μm filter by drawing through 5-10 mL of sterile H 2 O and discard water. Sterilize the D-Luciferin solution through the prepared 0.22 μm syringe filter. |
WGK Germany | 3 |
HS Code | 29342000 |
Reference Show more | 1. [IF=8.456] Chuchu Zhou et al."Alternative and Injectable Preformed Albumin-Bound Anticancer Drug Delivery System for Anticancer and Antimetastasis Treatment."Acs Appl Mater Inter. 2019;11(45):42534–42548 |
Introduction | D-fluorescein potassium salt is a water-soluble substrate of luciferase and exists in a variety of luminescent organisms. Under the catalytic action of ATP and luciferase, potassium D-luciferin is oxidized to produce blue-green light (560nm). When the substrate is excessive, the number of light quanta produced is positively correlated with the concentration of luciferase. Luc gene encoding luciferase is a common reporter gene in plant and mammalian cells. Since there is no background interference, luciferase as low as 0.02 pg levels can be easily detected. |
Uses | D-fluorescein potassium salt is a class of heterocyclic compounds found in organisms that can cause bioluminescence, such as fireflies. In the presence of ATP, luciferase will emit light after oxidative decarboxylation. Substrates that can be used for luciferase in chemical research. imaging analysis of in vivo/in vitro expression of uc marker gene and luciferase-fusion gene in living cells, tissues or organisms; 2) widely used in reporter gene analysis, immunoassay and ATP fluorescence hygiene monitoring analysis; |
biological activity | D-Luciferin is the substrate of firefly luciferase. |