GSK-3BETA INHIBITOR XII
TWS119
CAS: 601514-19-6
Molecular Formula: C18H14N4O2
GSK-3BETA INHIBITOR XII - Names and Identifiers
GSK-3BETA INHIBITOR XII - Physico-chemical Properties
| Molecular Formula | C18H14N4O2 |
| Molar Mass | 318.33 |
| Density | 1.42 |
| Melting Point | 249 °C |
| Appearance | powder |
| Color | white to beige |
| pKa | 8.99±0.10(Predicted) |
| Storage Condition | 2-8°C |
| In vitro study | Using 1μM TWS119 to treat P19 cells, 30-40% of the cells were differentiated, especially into nerve cell spectrum, according to the correct neuron morphology (by standard EB formation method, accompanied by TWS119 treatment, produce 60% neural differentiation) count TuJ1 positive cells. TWS119 binds tightly to GSK-3β(K D = 126 nM), quantified by surface plasmon resonance (SPR), with an IC50 of 30 nM. TWS119 acts on mouse embryonic carcinoma and ES cells and effectively induces neural differentiation. TWS119 treatment of hepatic stellate cells (HSC) reduced β-catenin phosphorylation, Induced β-catenin nuclear translocation, increased glutamine synthetase production, hindered the synthesis of smooth muscle actin and Wnt5a, but promoted glial protein acidic protein, expression of Wnt10b, and paired homeodomain transcription factor 2c. TWS119 triggers the rapid accumulation of β-catenin, enhances the nuclear protein interaction with oligonucleotides (DNA sequences containing Tcf and Lef binding), and greatly up-regulates Tcf7, Lef1 and other Wnt target genes including Jun, ezd7 (encoding Frizzled-7), Nlk (encoding Nemo-like kinase). TWS119 increases T-cell specific lethality and IFN-g release associated with protective IL-2 production capacity. Recent studies have shown that TWS119 treatment polyclonal activates human T cells and induces Wnt signaling. These T cells have the native CD45RA(+)CD62L(+) phenotype compared to control activated T cells expressing the CD45RO(+)CD62L(-) effector phenotype, this effect was TWS119 dose-dependent. |
| In vivo study | TWS119 was dosed at 30 mg/kg on the cell surface, and the cells expressed low levels of CD44 and high levels of CD62L. |
GSK-3BETA INHIBITOR XII - Preparation solution concentration reference
| 1mg | 5mg | 10mg | |
|---|---|---|---|
| 1 mM | 3.141 ml | 15.707 ml | 31.414 ml |
| 5 mM | 0.628 ml | 3.141 ml | 6.283 ml |
| 10 mM | 0.314 ml | 1.571 ml | 3.141 ml |
| 5 mM | 0.063 ml | 0.314 ml | 0.628 ml |
Last Update：2024-01-02 23:10:35
GSK-3BETA INHIBITOR XII - Reference Information
| biological activity | TWS119 is a GSK-3β inhibitor with IC50 of 30 nM, which can induce the differentiation of nerve cells, contribute to the study of stem cell biology. TWS119 is a GSK-3β inhibitor with an IC50 of 30 nM in a cell-free assay; It can induce differentiation of nerve cells and contribute to the study of stem cell biology. Inhibition of GSK-3β can be triggered by autophagy. |
| Target | Value |
| GSK-3β (Cell-free assay) | 30 nM |
Last Update：2024-04-10 22:29:15
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Product Name: Tofacitinib Impurity 269 Visit Supplier Webpage Request for quotationCAS: 601514-19-6
Tel: 0714-3999186
Email: 2853786052@qq.com
Mobile: 86+15671228036
QQ: 2853786052
Wechat: 15671228036
Product Name: TWS119 Visit Supplier Webpage Request for quotation
CAS: 601514-19-6
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Wechat: 18301782025
CAS: 601514-19-6
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Wechat: 18301782025
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