function | ginsenoside RA2 is the main active ingredient of medicinal plants such as ginseng and American ginseng, it has a wide range of pharmacological effects and medical uses. Ginsenoside has the effects of improving human immunity, promoting substance metabolism, anti-tumor, anti-fatigue, anti-photoaging, anti-oxidation, scavenging free radicals and immune stimulation. |
preparation | ginseng 5kg, add water decoction extraction three times, each time the amount of water is ginseng weight 12, 10, 8 times, decoction time was 2, 1.5, 1 hours, combined with decoction, D4020 macroporous adsorption resin adsorption, washed with water, ginsenoside Rg1 and ginsenoside Rb1 could not be detected in the eluate by 85% ethanol elution (silica gel thin layer chromatography, n-butanol: ethyl acetate: Water = 4: 1: 5, upper layer, 10% sulfuric acid spray, the mixture was heated at 105 ° C. For color development, and the eluate was recovered with ethanol to obtain 351g of total ginsenoside. Take 10g of total ginsenoside, dissolve with water, then put on alumina column, first wash with water until ginsenoside Re and ginsenoside Rg1 can not be detected in the eluate, the eluate was Re-adsorbed by AB-8 macroporous adsorption resin, eluted with ethanol, and the solvent was recovered under reduced pressure to obtain 3.1g of ginsenoside in triol group. TLC examination showed that the main components were ginsenoside Re and ginsenoside Rg1. It was then eluted with a 50% aqueous tetrahydrofuranide solution until no ginsenoside Rb1 was detected in the eluate. The solvent was recovered from the eluate under reduced pressure to obtain 5.2g of diol-group ginsenoside. The main components were ginsenoside Ra1, ginsenoside RA2, ginsenoside Ra3, ginsenoside Rb1, ginsenoside Rb2, ginsenoside Rb3, ginsenoside Rc, Ginsenoside Rd, etc. |