LIVE/DEAD Bacterial Viability and Counting Kit, for flow cytometry
LIVE/DEAD Bacterial Viability and Counting Kit, for flow cytometry
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LIVE/DEAD Bacterial Viability and Counting Kit, for flow cytometry - Names and Identifiers
LIVE/DEAD Bacterial Viability and Counting Kit, for flow cytometry - Physico-chemical Properties
| Storage Condition | 2-8 ℃, 12 months |
LIVE/DEAD Bacterial Viability and Counting Kit, for flow cytometry - Reference
| Reference Show more | 1. [IF=8.128] Xiangyong Liu et al."Establishment of anti-oxidation platform based on few-layer molybdenum disulfide nanosheet-coated titanium dioxide nanobelt nanocomposite."J Colloid Interf Sci. 2021 Nov;601:167 2. [IF=7.963] Fang Guan et al."Synergistic effect of carbon starvation and exogenous redox mediators on corrosion of X70 pipeline steel induced by Desulfovibrio singaporenus."Sci Total Environ. 2021 Sep;788:147573 3. [IF=6.843] Shilu Ji et al."An oxygen-sensitive probe and a hydrogel for optical imaging and photodynamic antimicrobial chemotherapy of chronic wounds."Biomater Sci-Uk. 2022 Apr;10(8):2054-2061 4. [IF=15.881] Xinyuan Yang et al."Physiologically Inspired Mucin Coated Escherichia coli Nissle 1917 Enhances Biotherapy by Regulating the Pathological Microenvironment to Improve Intestinal Colonization."Acs Nano. 2022;XXXX(XXX):XXX-XXX |
LIVE/DEAD Bacterial Viability and Counting Kit, for flow cytometry - Introduction
Live dead bacteria staining kit (LIVE/DEAD Bacterial Viability and Counting Kit, for flow cytometry) can effectively distinguish live and dead bacteria and realize quantitative detection by flow cytometry. Moreover, quantitative analysis and detection can also be realized for the mixture of various bacteria. The kit uses two nucleic acid dyes to determine the bacterial activity: green fluorescent dye (functionally equivalent to SYTO 9) and red fluorescent dye PI (propidium iodide), and accurately measure the sample volume through standard microspheres. When stained with the appropriate proportion of green fluorescent dye and PI mixture, the bacteria with intact cell membrane emit bright green fluorescence, while the bacteria with damaged cell membrane show obvious weakening of green fluorescence and strong red fluorescence. Cell type and Gram characteristics affect the intensity of red fluorescence production by dead bacteria. Green fluorescent dye and PI dye are effectively excited by 488nm laser in most flow cytometry. The fluorescence signal of nucleic acid complex can be detected in green channel and red channel respectively, and there is almost no background fluorescence. The standard microsphere suspension is used as the reference standard for sample volume. The size and fluorescence are carefully screened to ensure that the fluorescence of the microspheres in flow cytometry-the lateral scattering diagram can be clearly distinguished from the stained bacteria.
Last Update：2022-10-16 17:40:27
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Product Name: LIVE/DEAD Bacterial Viability and Counting Kit, for flow cytometry Visit Supplier Webpage Request for quotation
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Wechat: 18301782025
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Wechat: 18301782025
Product Name: LIVE/DEAD Bacterial Viability and Counting Kit, for flow cytometry Visit Supplier Webpage Request for quotation
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Wechat: 18301782025
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Wechat: 18301782025
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