Name | NcmECL Ultra |
Reference Show more | Note: part of our products can only provide part of the information, our company does not guarantee the authority of the information provided, only for the customer reference Exchange research. use: This product is for scientific research only, not for other purposes. storage conditions: 2-8 ℃ ■ High Sensitivity: up to flying gram level, 10-15g.■ high safety: the chemical substances in the reagent are safe and do not contain toxic compounds to human health ■ long signal duration: Luminescence time up to more than 30 minutes ■ high stability: 4 ℃ storage for more than one year, good transport stability at room temperature ■ cost-effective: the cost is lower than that of imported products, the required antibody is less, the antibody dilution is high NcmECL Ultra®It is a high-sensitivity ECL chemiluminescence kit, which is used to detect the HRP (horseradish peroxidase) labeled target band, and the detection sensitivity is up to fg level. NcmECL Ultra optimizes the composition of the substrate, applies a new type of Enhancer and oxidant, has higher luminous intensity, better stability, does not contain toxic reagents, and has higher safety. Introduction |
Introduction | high-sensitivity ECL chemiluminescence kit is a chemiluminescence system, also known as high-sensitivity ECL luminescent solution, it is used for detecting proteins or nucleic acids immobilized on the membrane, has high sensitivity, low background, and the detection sensitivity can reach high femtogram level, and is suitable for detecting trace proteins or nucleic acids. The principle: the hypersensitive ECL chemiluminescent substrate kit is used to detect antibodies, antigens or nucleic acids that directly or indirectly label horseradish peroxidase (HRP). After electrophoresis, the protein or nucleic acid is transferred to the imprinted membrane, and the HRP labeled antibody or probe is bound to the target protein or nucleic acid on the membrane. After washing the membrane, it is placed in the ECL working solution prepared with this product, after incubation for several minutes at room temperature, HRP oxidizes the Luminol in the working solution and emits light, which can be greatly enhanced by the addition of an enhancer in the reagent. This light can be recorded by X-ray film or electronic device, and the protein or nucleic acid band can be clearly displayed. |
Use | high-sensitivity ECL chemiluminescence kit is a chemiluminescence system, it is used for detecting proteins or nucleic acids immobilized on the membrane, has high sensitivity, low background, and the detection sensitivity can reach high femtogram level, and is suitable for detecting trace proteins or nucleic acids. The luminescence signal is long-lasting, and the unique formula is sufficient to maintain the luminescence for more than 6 hours, which is convenient for repeated exposure operation. |