| Use | DNase is often used as an expectorant in clinic. Because this product can make the antibacterial easy to reach the infection focus, give full play to the role of antibiotics, so it is especially suitable for respiratory system infection after the production of a large number of pus sputum, so that the sputum is thin and easy to cough. It can also be used locally for abscesses, hematomas, and intrathecal injections to treat meningitis. But after injection may cause gastrointestinal reaction, weakness, occasional wind rash. Acute suppurative cellulitis, bronchial thoracic fistula in patients with active tuberculosis use. Compatibility with heparin and citrate is prohibited. Spray, 50000~100000 units each time, 3~4 times a day, continuous medication for 4~6 days. Intravenous or intramuscular injection, 1 million units each time. Intrathecal injection, 1 million units each time. |
| production method | , bovine pancreas was extracted with 0.125mol/L H2SO4 → extracted extract salting-out extract (NH4)2SO4 → Salting-out precipitate H2O → dissolved solution (NH4)2SO4 → Salting-out precipitation grade salting-out precipitation H2O → dissolved solution (NH4)2SO4 → grade salting-out precipitation dialysis, filtration, freeze-drying precipitation H2O → Dissolution; Dialysis dialysate filtration; Freeze-drying → finished product method 2, seed culture and fermentation of β-hemolytic chain homolog medium with β-hemolytic chain bacterium as raw material → tertiary seed culture tertiary seed culture broth fermentation medium → fermentation broth salting-out, elution of fermentation broth cholesterol ethanol; 724 resin; PH 7.5~5 → Salting-out adsorbate H2O;NaOH;PH 7.2 → elution eluent salting-out and precipitation eluent (NH4)2SO4;PH 7;100C → Salting-out mother liquor (NH4)2SO4;PH 4.6 → precipitated precipitate calcium phosphate treatment, precipitated precipitate (Na3)PO4;Ca(Ac)2,ph7.2 → calcium phosphate treatment enzyme solution ice methanol; ph5 → precipitated enzyme crude product grade precipitation, salting-out enzyme crude product (NH4)2SO4;ph6.2 → 4.6 → fractionally precipitated precipitate ^ H2OnaCl;ph7.2 → 4.6 → 2.5 → isoelectric point treatment of salting-out precipitate and precipitated precipitate H2O; PH 2.5 → → isoelectric point treatment enzyme solution ice methanol; Ph; 0 ~ 80C → precipitate dissolution, sterile treatment precipitate DEAE cellulose; phosphate buffer solution → aseptic processing of enzyme refined solution → finished product |