Oxidase, ascorbate
Ascorbate Oxidase
CAS: 9029-44-1
Molecular Formula: N/A
Oxidase, ascorbate - Names and Identifiers
| Name | Ascorbate Oxidase |
| Synonyms | ASCORBASE EC 1.10.3.3 Ascorbate Oxidase ASCORBATE OXIDASE Oxidase, ascorbate Ascorbate Oxidase, Cucurbita sp. ASCORBATE OXIDASE CUCURBITA SPECIES |
| CAS | 9029-44-1 |
| EINECS | 232-852-6 |
Oxidase, ascorbate - Physico-chemical Properties
| Molecular Formula | N/A |
| Molar Mass | 67KDa(SDS-PAGE) |
| Solubility | Soluble in aqueous buffers. |
| Appearance | powder |
| Color | faintly bluish |
| Storage Condition | 2-8°C |
| MDL | MFCD00130560 |
| Physical and Chemical Properties | Ascorbate oxidase (AO), short for ASO, belongs to ceruloplasmin family, molecular weight of 140kD, composed of two identical subunits (70kD), can be ascorbic acid oxidation into dehydroascorbic acid, at the same time, with oxygen to generate water, coupled with other redox reactions, it plays a role of terminal oxidase, which can catalyze the oxidation of ascorbic acid, and has anti-aging effect, it plays an important role in the metabolism of substances in plants. AO in Cucurbitaceae plants (cucumber, pumpkin, gourd, etc.) in the content is higher, so the separation and purification of the enzyme is mainly extracted from these plants. |
| Use | Remove ascorbate. |
Oxidase, ascorbate - Risk and Safety
| Safety Description | 24/25 - Avoid contact with skin and eyes. |
| WGK Germany | 3 |
| FLUKA BRAND F CODES | 10-21 |
| HS Code | 35079090 |
Oxidase, ascorbate - Reference
| Reference Show more | 1. Wang, Y., Yang, Y., Liu, W. et al. A carbon dot-based ratiometric fluorometric and colorimetric method for determination of ascorbic acid and of the activity of ascorbic acid oxidase. Microchim Acta 186, 246 (2019). https://doi.org/10.1007/s00604-019-3341- 2. [IF=6.992] Li Guangjie et al."The Arabidopsis AMOT1/EIN3 gene plays an important role in the amelioration of ammonium toxicity."J Exp Bot. 2019 Feb;70(4):1375-1388 3. [IF=5.833] Wang Yanying et al."A carbon dot-based ratiometric fluorometric and colorimetric method for determination of ascorbic acid and of the activity of ascorbic acid oxidase."Microchim Acta. 2019 Apr;186(4):1-10 4. [IF=3.223] Ke Yang et al."Using Transparent Adhesive Tape as New Substrate for Integrated Flexible Enzymatic Sensor: Good Adhesion and Better Printability."Electroanal. 2021 Jun;33(6):1668-1677 5. [IF=4.098] Guo-Ying Chen et al."Highly sensitive visual colorimetric sensor for trichlorfon detection based on the inhibition of metallization of gold nanorods."Spectrochim Acta A. 2022 Apr;270:120850 |
Oxidase, ascorbate - Trait
White or off-white or slightly copper-blue crystalline powder. Belongs to the ceruloplasmin family, molecular weight of 140kD, composed of two identical subunits (70kD). Is an oxidoreductase. Catalyze the reaction of ascorbic acid with oxygen to form dehydroascorbic acid. Ascorbic acid is an unsaturated polyhydroxy compound, in the form of Ester, in the 2 and 3 carbon atoms between the hydroxyl group of the hydrogen alcohol can be free to H, it is acidic
Last Update：2023-08-16 21:32:38
Oxidase, ascorbate - Vitality definition
One unit causes the decrease of one micromole of ascorbic acid per minute at pH5.6 at 30℃
Last Update：2023-08-16 21:32:38
Oxidase, ascorbate - Vitality
≥400units/mg protein
Last Update：2023-08-16 21:32:38
Oxidase, ascorbate - Optimal PH
6.0
Last Update：2023-08-16 21:32:38
Oxidase, ascorbate - Optimum temperature
50℃
Last Update：2023-08-16 21:32:38
Oxidase, ascorbate - Inhibitors
Ag+，Hg2+
Last Update：2023-08-16 21:32:38
Oxidase, ascorbate - pH stability
6.0～11.0（25℃，20hr）
Last Update：2023-08-16 21:32:38
Oxidase, ascorbate - Reference Information
| copper-containing enzyme | ascorbate oxidase is a copper-containing enzyme found in melons, seeds, grains, fruits and vegetables. It can oxidize ascorbic acid to produce water and dehydroascorbic acid. Compared with non-enzymatic oxidation, ascorbate oxidase produces water, while the former produces hydrogen peroxide. The existence of ascorbate oxidase is only a "defense system" of the plant itself. So far, there is no scientific evidence that it can oxidize vitamin C in food. |
| Use | Used to add fish products and other foods Biochemical research |
Last Update：2024-04-09 20:52:54
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