Polysacharid lentinus edodes
Lentinan
CAS: 37339-90-5
Molecular Formula: (C42H70O35)n
Polysacharid lentinus edodes - Names and Identifiers
| Name | Lentinan |
| Synonyms | LC 33 LC-33 LENTINAN Lentinan DRG-0171 Bromoduline biomoduline LENTINEX(R) lentinan vial Polysacharid lentinus edodes LENTINAN (SHIITAKE MUSHROOM POLYSACCHARIDES) Lentinan [BioModuline |
| CAS | 37339-90-5 |
| EINECS | 1806241-263-5 |
| InChI | InChI=1/C42H72O36/c43-1-8-15(48)22(55)25(58)37(69-8)66-6-13-20(53)32(28(61)36(65)68-13)75-40-29(62)33(18(51)11(4-46)72-40)77-41-30(63)34(19(52)12(5-47)73-41)78-42-31(64)35(76-39-27(60)24(57)17(50)10(3-45)71-39)21(54)14(74-42)7-67-38-26(59)23(56)16(49)9(2-44)70-38/h8-65H,1-7H2/t8-,9-,10-,11-,12-,13-,14-,15-,16-,17-,18-,19-,20-,21-,22+,23+,24+,25-,26-,27-,28-,29-,30-,31-,32+,33+,34+,35+,36-,37-,38-,39+,40+,41+,42+/m1/s1 |
Polysacharid lentinus edodes - Physico-chemical Properties
| Molecular Formula | (C42H70O35)n |
| Molar Mass | 1153 |
| Density | 1.88g/cm3 |
| Boling Point | 1472°C at 760 mmHg |
| Specific Rotation(α) | D20 +13.5-14.5° (in 2% NaOH); +19.5-21.5° (in 10% NaOH) |
| Flash Point | 844°C |
| Solubility | 30mg/ml H2O clarified to turbidity |
| Vapor Presure | 0mmHg at 25°C |
| Appearance | Brown block solid |
| Storage Condition | under inert gas (nitrogen or Argon) at 2-8°C |
| Refractive Index | 1.711 |
| MDL | MFCD01773536 |
| Physical and Chemical Properties | White powder, decomposed at 250 °c. Odorless or slightly odorous, tasteless. Soluble in alkali solution or formic acid, soluble in hot water or dimethyl sulfoxide, insoluble in cold water, alcohol, ether, chloroform, pyridine or hexamethylphosphoramide. Stable against sulfuric acid and hydrochloric acid. [Α] D20 +13.5 ° ~ +14.5 °(2% sodium hydroxide);[α]D20 +19.5 ° ~ +21.5 °(10% sodium hydroxide). |
Polysacharid lentinus edodes - Reference
| Reference Show more | 1. Xia LianCHEN cultivates Jiao Wenjia, etc. Preparation of lentinan embedded with W/O/W multiple milk adjuvants [J]. Journal of Wuhan Polytechnic University 2019 038(002):1-8. 2. Wang Xiaomin Xuanjin Lu Fangguo et al. Effects of polysaccharides from Curculigo orchioides on the secretion of several active factors by macrophages [J]. Chinese national and folk medicine 2017 026(007):32-34 38. 3. Zhu Yanan, Xu Yiyang, Ding Huiming, et al. Immunomodulatory effects of polysaccharides from Pleurotus nebrodensis on immunosuppressed mice [J]. Science and Technology of food industry, 2020(7):295-300. 4. Wan Qianlin, Ren Yuhe, LV Rena, et al. Effects of lentinan on proliferation, cell cycle, apoptosis and migration of glioma SHG-44 cells [J]. Chinese traditional medicines, 2019, 041(011):2614-2619. 5. Han Lili, Wu Fei, Huang Lanxuan, Zhang Shuqun. Reversal effect and mechanism of lentinan on sorafenib resistance in hepatocellular carcinoma cells [J]. Journal of Xi'an Jiaotong University (Medical Sciences),2021,42(01):48-52 64. 6. Wang Xiaolan, Duan Pengfei, Yang Meng, Zheng Xiaoke, Feng hygienic. Immunomodulatory effects of rehmannia glutinosa polysaccharide on cyclophosphamide-induced immunosuppression in mice [J]. Journal of Shanghai University of Traditional Chinese Medicine, 2021,35(01):55-60 92. 7. He, Jin, et al. "Immune-adjuvant activity of lentinan-modified calcium carbonate microparticles on a H5N1 vaccine." International Journal of Biological Macromolecules 163 (2020): 1384-1392.https://doi.org/10.1016/j.ijbiomac.2020.08.005 8. Chen, Zhiqiu, et al. "Effects of polysaccharide from the fruiting bodies of Auricularia auricular on glucose metabolism in 60Co-γ-radiated mice." International journal of biological macromolecules 135 (2019): 887-897.https://doi.org/10.1016/j.ijbiomac.2019 9. Wei L. I. U., Y. A. N. Ran, and Liang Zhang. "Dendrobium sonia polysaccharide regulates immunity and restores the dysbiosis of the gut microbiota of the cyclophosphamide-induced immunosuppressed mice." Chinese journal of natural medicines 17.8 (2019): 600 10. [IF=0.252] Huan-Long Xu et al."Lentinan up-regulates microRNA-340 to promote apoptosis and autophagy of human osteosarcoma cells."Int J Clin Exp Patho. 2018; 11(8): 3876-3883 11. [IF=9.381] Zhenguang Liu et al."Preparation of lentinan-calcium carbonate microspheres and their application as vaccine adjuvants."Carbohyd Polym. 2020 Oct;245:116520 12. [IF=9.381] Duanhua Cai et al."A carboxymethyl lentinan layer by layer self-assembly system as a promising drug chemotherapeutic platform."Carbohyd Polym. 2021 Jun;261:117847 13. [IF=8.758] Zhenguang Liu et al."Lentinan-Functionalized Graphene Oxide Is an Effective Antigen Delivery System That Modulates Innate Immunity and Improves Adaptive Immunity."Acs Appl Mater Inter. 2020;12(35):39014-39023 14. [IF=6.953] Zhiqiu Chen et al."Effects of polysaccharide from the fruiting bodies of Auricularia auricular on glucose metabolism in 60Co-γ-radiated mice."Int J Biol Macromol. 2019 Aug;135:887 15. [IF=6.953] Yiyu Chen et al."Structural characterization and biological activities of a novel polysaccharide containing N-acetylglucosamine from Ganoderma sinense."Int J Biol Macromol. 2020 Sep;158:1204 16. [IF=6.331] Lei Bai et al."Surface modification engineering of two-dimensional titanium carbide for efficient synergistic multitherapy of breast cancer."J Mater Chem B. 2020 Aug;8(30):6402-6417 17. [IF=5.81] Yin Huifang et al."Protective Effects of Lentinan Against Lipopolysaccharide-Induced Mastitis in Mice."Front Pharmacol. 2021 Sep;0:2559 18. [IF=5.162] Jin He et al."Immune-adjuvant activity of lentinan-modified calcium carbonate microparticles on a H5N1 vaccine."Int J Biol Macromol. 2020 Nov;163:1384 19. [IF=3] Wei LIU et al."Dendrobium sonia polysaccharide regulates immunity and restores the dysbiosis of the gut microbiota of the cyclophosphamide-induced immunosuppressed mice."Chin J Nat Medicines. 2019 Aug;17:600 20. [IF=2.447] Yun Gui et al."Oridonin improves the therapeutic effect of lentinan on lung cancer."Exp Ther Med. 2021 Aug;22(2):1-9 21. [IF=6.576] Xiaoying Yang et al."Lentinan Supplementation Protects the Gut-Liver Axis and Prevents Steatohepatitis: The Role of Gut Microbiota Involved."Front Nutr. 2021; 8: 803691 |
Polysacharid lentinus edodes - Nature
Open Data Verified Data
- is a white powdery solid that is stable to light and heat. The maximum solubility in water is 3 mg/mL; Can be dissolved in 0. 5mol/L sodium hydroxide, solubility up to 50 ~ lOOmg/mL; Insoluble in methanol, ethanol, acetone and other organic solvents. Lentinan has hygroscopicity, in the relative humidity of 92. 5% 25 ℃ room temperature environment for 15 days, water absorption can reach 40%. Lentinan is a polar macromolecular compound, and its specific structure is closely related to immune activity. Therefore, the extraction of lentinan mostly uses different temperatures of water and dilute alkali solution, and try to avoid operating under too acidic conditions, because the strong acid properties cause the breaking of the polyglycosidic bond.
- The polysaccharide obtained by this strain from the fruiting body or mycelium cultured in submerged fermentation of the mushroom [lentis Dodes (Berk) Sing] is p(i-3) as the main chain (1-6) it is a high molecular weight D-glucan with side chains. Its anti-tumor effect is mediated by thymus-dependent immune mechanism. The molecular weight of extracellular polysaccharide is about 1 million, which can stimulate the function of T cells. The molecular weight of intracellular polysaccharide is 60,000~100,000, which induces the industry of interferon and is related to the activation of giant cells. Lentinan can affect a variety of immune functions of the body, such as: promoting the function of mononuclear phagocyte system; Promoting lymphocyte proliferation; Promoting the production of macrophages by IL-1; enhanced delayed type hypersensitivity (DTH) to sheep red blood cells (SRBC). Partial or complete recovery of DTH inhibition by tumor-bearing or immunosuppressant injection; Enhancement of antibody-dependent cytotoxicity (ADCC) in mouse splenocytes, etc. It mainly affects Th cells and Tc cells, and can restore the activity of Th and Tc cells inhibited in humans and animals. Its mode of action may be that it promotes the production of IL-1, which activates T cells, and clonal stimulator (CSF), which is produced by activated T cells, Refeedback acts on macrophages, increasing production by IL-1, and further activates the immune system. Lentinan has obvious protective effect on experimental liver injury, which can obviously recover the increase of serum alanine aminotransferase and the decrease of liver glycogen induced by carbon tetrachloride.
Last Update：2024-01-02 23:10:35
Polysacharid lentinus edodes - Preparation Method
Open Data Verified Data
there are conventional extraction method and compound enzymatic hydrolysis extraction method.
- conventional extraction method. Take fresh fruit body of Lentinus edodes, wash clean, add 5 times the amount of boiling water to soak 8~15h, filter, filtrate decompression concentration. The concentrated solution was added with 1-fold amount of ethanol to generate a precipitate, which was filtered, and the filtrate was added with 3-fold amount of ethanol to obtain a precipitate. Under vigorous stirring, a solution of 0. 2mol/L sodium hydroxide in decahexyltrimethylammonium was added dropwise, and a large amount of precipitate was generated when the pH value was gradually adjusted to 12.8. The precipitate was centrifuged, and then washed with ethanol to collect the precipitate. Then the precipitate was washed with chloroform and n-butanol to remove the protein, the water layer was precipitated with 3 times the amount of ethanol, and the precipitate was collected. Then the precipitate was washed with methanol and ether, and dried in a vacuum dryer, that is, lentinan.
- compound enzymatic hydrolysis extraction method. Take dried Lentinus edodes, mash, add 6-7 times the amount of water to mix, heat to 90 ℃, stir for 60min, cool to 40 ℃, transfer to 40 ℃ water bath for heat preservation, and add appropriate amount of mixed hydrolase solution, the pH was 6.4 and the reaction was stirred for 60min. Then rapidly heated to 90~100 ℃, inactivated 1min, after cooling gauze filtration, the remaining steps with conventional extraction method.
Last Update：2022-01-01 11:13:37
Polysacharid lentinus edodes - Use
Open Data Verified Data
- lentinan has the effects of anti-tumor, lowering cholesterol, inhibiting transaminase activity and platelet aggregation. The toxic and side effects of lentinan are negligible compared with the usual chemotherapy drugs. With chemotherapy drugs and used in patients with gastrointestinal cancer, can prolong the survival time, prevent leukopenia or serum total protein decreased. Chronic hepatitis B has a certain effect, can significantly improve the patient's symptoms, the serum alanine aminotransferase to normal or improved, and can adjust the patient's immune function. Also used for anti-radiation, anti-diabetes, etc. In recent years also reported that lentinan derivatives can be used as a treatment of AIDS drugs.
- usage and dosage for tumor, 2mg intravenous injection, once a week. For viral hepatitis, lentinan, oral, each 12. 5mg, 2 times a day.
- preparation and specification freeze-dried powder for injection, 1 mg/vial; Injection, 4mg/vial; Tablet: 2.5mg/tablet.
Last Update：2022-01-01 11:13:38
Polysacharid lentinus edodes - Safety
Open Data Verified Data
- anaphylactic shock and decreased white blood cells and hemoglobin may occasionally occur. Sometimes loss of appetite, Nausea, Vomit, chest tightness, shortness of breath, Head Pain, dizziness, head weight and rash, sweating, Fever and other discomfort reaction. Children and pregnant women and women of childbearing age should be used with caution; Pay special attention to the allergic shock of this product, once the occurrence of chills, abnormal pulse, blood pressure drop, abnormal sense of mouth, Dyspnea, etc., should be discontinued immediately, and give emergency treatment; Avoid mixing with Vitamin V and vitamin A, which will make the injection turbid; This product should be dissolved in normal saline or 5% glucose solution, should be used immediately, should not be stored for a long time.
- room temperature storage.
Last Update：2022-01-01 11:13:39
Polysacharid lentinus edodes - Reference Information
| source | lentinan is a kind of high molecular glucan with anti-cancer effect extracted from lentinan entity. its polysaccharide part is mainly mannose, and there are glucose, galactose, xylose, arabinose, etc. the peptide chain is composed of 18 kinds of amino acids such as aspartic acid, histidine, serine and glutamic acid. [Source Plant] Lentinus edodes is the fruiting body of the fungus Lentinus edodes of the genus Lentinus edodes. Lentinus edodes is rich in nutrition. Every 100 grams of dried product contains 13 grams of protein, 1.8 grams of fat, 54 grams of carbohydrates, 7.8 grams of crude fiber, and a variety of vitamins. It contains more vitamin D and adenine, which has a preventive effect on the occurrence of rickets and liver cirrhosis. Lentinus edodes contains lentinan, which can inhibit the proliferation of sarcoma S-180 in mice and has certain prospects in the development of natural anticancer drugs. The artificial cultivation of shiitake mushrooms began in China. Wang Zhen's "Nongshu" in Yuan Dynasty recorded the cultivation method of shiitake mushrooms in detail. This natural spore inoculation and cultivation method has been used in China, Japan and other countries until modern times. In 1928, Japan Morimoto Hikaburo successfully cultured pure mushrooms with isolation medium. Later, Japan gradually promoted the artificial inoculation and cultivation of pure strains. This technology was introduced to China in the 1930s and began to be widely used in the mid-1960s. During the same period, Shanghai Edible Fungus Research Institute and other units tried sawdust to cultivate shiitake mushrooms, which was gradually popularized and applied in the 1970s, making substitute cultivation a cultivation method with Chinese characteristics. Shiitake mushrooms rank second in the world's total mushroom production. Japan has the largest output of shiitake mushrooms. In 1987, China's output of dried shiitake mushrooms exceeded 10,000 tons, ranking second in the world. [morphology] the cap is semi-fleshy, 5~12cm in diameter, oblate hemispherical, gradually spreading back, yellowish brown to dark cinnamon, with scales on it. The flesh is thick, white, and the gills are white, dense and curved. From native to partial, white, internal solid, often curved, 3~5cm long, 0.5~0.8cm in diameter, the part below the fungus ring is often covered with scales. The fungus ring is narrow and easy to disappear (Figure 1). The spores are colorless and smooth. Oval, 5 × 2.5 microns. Figure 1 shows shiitake mushrooms. |
| Polysaccharides | Any sugar that can be hydrolyzed into multiple monosaccharide molecules or their derivatives is a polysaccharide. It is a macromolecular compound formed by condensation and dehydration of 20 monosaccharides to tens of thousands of monosaccharides. Polysaccharides can be condensed from a monosaccharide, such as starch, cellulose, etc. to become homopolysaccharides; they can also be condensed from different monosaccharides or their derivatives, such as hemicellulose, which is called heteropolysaccharides. Plants, animals and microorganisms in nature all contain polysaccharides. In addition to the free state, polysaccharides can also exist in a bound form with proteins. The commonality of plant polysaccharides is that they have natural properties, health care value and unique water-soluble colloid properties, so they can give food high nutritional value, stable and palatable tissue structure, exquisite appearance and unique taste, and extend shelf life. Health function activity of polysaccharide: 1. Anti-tumor. For example, lentinan obtained from Lentinus edodes has strong anti-tumor activity. 2. Enhance immune function. Different polysaccharides have different immune promoting effects. Lentinan is an ideal immune promoter. Tremella polysaccharide can promote the transformation of lymphocytes, enhance the phagocytic function of mouse peritoneal macrophages, significantly promote the synthesis of liver protein and nucleic acid, promote bone marrow hematopoietic function, and improve humoral immunity. Auricularia auricula polysaccharide can effectively improve the phagocytic index and percentage of mouse macrophages. Polysaccharides extracted from garlic and radish have different enhancing effects on T lymphocytes, B lymphocytes and macrophages respectively, which can enhance the cellular immune function of the body. 3. Lowering blood sugar and blood lipid. The polysaccharide with hypoglycemic effect is milk glucan. Lentinan has the effect of dissolving cholesterol. Auricularia auricula polysaccharide has the effect of relieving atherosclerosis. |
| research history | In 1969, Japan's Chihara Kuro first used hot water to extract 6 kinds of extracellular polysaccharides from the fruiting body of Lentinus edodes, of which 2 have obvious anti-tumor activity, with an average relative molecular mass of 1000000, belonging to branched β(1 → 3) glucan. in 1970, Chihard and others extracted 4 kinds of polysaccharides from shiitake mushrooms by hot water extraction and organic solvent precipitation, of which 2 kinds of polysaccharides have anti-tumor effect. in the early 70s, Shida et al. extracted three water-soluble polysaccharides from Lentinus edodes by trichloroacetic acid extraction, methanol precipitation and other methods, one of which was composed of Gal,L-Fuc and Man (molar ratio 6:1.5:1). this is another anti-tumor substance reported for the first time in Lentinus edodes except β-glucan. Sasaki also reported that besides the macromolecule lentinan with a relative molecular mass of 1 million, another small molecule lentinan with a relative molecular mass of 16000 also has anti-tumor activity. Since the 1980s, the research on lentinan has been more in-depth. For example, Schulty extract an intracellular polysaccharide from the hyphae of lentinus edodes, the relative molecular mass (6.0~9.5)× 104 is mannoside containing α-chain, accompanied by a small amount of peptide immunoactive substances composed of serine, threonine and alanine. In 1980, Cotoneaster II in Japan confirmed that lentinan was a broad-spectrum immune promoter. A large number of subsequent studies have shown that lentinan has anti-tumor effects, especially for chronic myeloid leukemia, gastric cancer, nasopharyngeal cancer, rectal cancer and breast cancer. It has the effect of inhibiting and preventing postoperative micrometastasis. This effect is through Enhancing the body's immunity and showing indirect toxicity to cancer cells, so it is especially suitable for the rehabilitation of patients. In 1984, a patent report in the United States was to culture Lentinus edodes with xylose-rich solid medium. From mycelial culture, an anti-tumor glycoprotein with xylose as the main component can be obtained. |
| Chemical structure | Although there are many reports on the chemical composition and structure of lentinan, only β-glucan has a definite immune activity relationship. The primary structure of lentinan has a main chain of glucopyranan connected by β(1 → 3) bonds. The glucose in the main chain is connected to the side chain through 6-C branches. Generally, there are 2 for every 5 glucose. The fulcrum, the side chain is a glucose low polymer connected by β(1 → 6) and β(1 → 3) bonds. Water-soluble β-glucan has a linear structure without large branches. The physiological activity of dextran with β(1 → 3) bond as the main structure is higher than that of polysaccharide with β(1 → 6) as the main structure, and the coexistence of side chains bound by β(1 → 3) and β(1 → 6) is required for anti-tumor effect. Lentinan has a triple helix three-dimensional structure. When this three-dimensional structure is destroyed by urea and DMSO, the immune activity disappears. Other polysaccharides in Lentinus edodes are composed of glucose, galactose, mannose, L-arabinose and xylose, and there are also α-mannosides derived from the mycelium of submerged fermented Lentinus edodes. In recent years, people have carried out research on chemical modification of lentinan to find more effective polysaccharide substances. For example, 10 μg/mL sulfated lentinan can effectively protect HIV-infected MT-4 cells, and 100 μg/mL can inhibit RT activity and the generation of synaptic cells. Its inhibitory effect on stress transcriptase is higher than that of sulfated dextran. Through 13C-NMR and other measurements, it is proved that the introduction of sulfuric acid base leads to the physical and chemical properties of lentinan and the conformational changes of the main body, resulting in the different immune activity of the derivatives. |
| Drug properties and applications | Lentinan is an immune enhancer and is an anti-tumor auxiliary drug. It has no direct anti-tumor effect, but through the activation of the host's disease prevention mechanism, including activation of cytotoxicity of killing T cells, macrophages, natural killer cells (NK) and antibody-dependent macrophages (ADMC), it also enables the inhibited helper T lymphocytes to restore function and increase the induction of interleukin and interferon in the blood to play a synergistic effect, which can further activate the immune system and have a significant protective effect on the liver. Single use or combined with other anti-tumor drugs can inhibit the proliferation of various animal tumors, especially when combined with tegafur (FT-207), the survival time of patients is significantly longer than that of single use. This product is distributed in the whole body organs after 5 minutes of intravenous injection, mainly distributed in the liver, followed by the spleen, lung, and kidney. Its blood drug concentration decreases rapidly, disappears slowly in liver and spleen, and decreases rapidly in lung and kidney. Mainly excreted from the urine, a small amount from the feces, almost not through the placenta, not into the milk. It is clinically used for adjuvant treatment of malignant tumors, such as inoperable or recurrent gastric cancer, liver cancer, and bladder cancer patients, to relieve clinical symptoms, improve immunity, and correct the imbalance of trace elements. Combined with FT-207 can prolong the survival time of patients. It can also be used for hepatitis B, liver poisoning, liver cirrhosis, etc. |
| extraction method | currently, hot water and dilute alkali solution are mostly used for extraction of lentinan, avoiding being carried out in strong acid and alkali solution, otherwise it is easy to cause glycosidic bond breakage and conformational change in polysaccharide. The commonly used separation and purification methods roughly go through the steps of boiling water extraction, ethanol precipitation, dialysis and column chromatography. Considering that there are other physiologically active ingredients in Lentinus edodes (such as adenine, tyrosine oxidase and vitamin D, etc.), the final product formula and extraction purpose should be clarified when determining the extraction method. Generally, the fruiting body of Lentinus edodes is crushed first, stirred and extracted with 90~100 ℃ water for 3~5h, repeated for 2~3 times, then extracted with 0.5mol/L NaOH solution, and the polysaccharide is preliminarily divided into water soluble and alkali soluble parts. Then remove the mushroom residue, the supernatant is dialyzed or ion exchange to remove small molecular impurities, and the crude product is obtained after precipitation with ethanol or thiamine. In the above extraction process, if the mushroom powder is treated with some compound enzyme preparations in advance, the yield of polysaccharide can be greatly improved. |
| adverse reactions | 1. central nervous system: occasional headache, dizziness and head weight. 2. Gastrointestinal tract: occasional nausea, vomiting and decreased appetite. 3. respiratory system: occasionally feel chest pressure or pharyngeal obstruction. 4. Blood system: occasionally red blood cells, white blood cells and hemoglobin decreased. 5. Skin: occasional rash and erythema. 6. Other: Occasionally fever, sweating, facial flushing and other symptoms, rare shock. |
| applicable food category | infants, children and people allergic to edible fungi are not suitable for eating. |
| approval date | 2014-05-20 |
| Announcement No. | Health and Family Planning Commission No. 10, 2014 |
| announcement title | announcement on approval of 6 new food ingredients such as tagatose |
| precautions | those who are allergic to this product are prohibited. there is no clinical experience in the safety of medication for children, the elderly, pregnant women and lactating women, and should be used with caution. The powder injection of this product should be strongly shaken when dissolved in sterilized water for injection, and then diluted with 5% glucose injection or 0.9% sodium chloride injection to adjust it to isotonic solution, and use it as soon as possible after preparation. |
| use | immune enhancer. For patients with inoperable or recurrent gastric cancer. Combined with chemotherapy drugs for patients with gastrointestinal tumors, it can prolong survival time and prevent leukopenia or serum total protein reduction. It has a certain effect on chronic viral hepatitis B, which can significantly improve the patient's symptoms, make the serum alanine aminotransferase normal or improved, and adjust the patient's immune function. Note: Anaphylactic shock, leukopenia and hemoglobin may occasionally occur. Children and women of pregnant and childbearing age should be used with caution. |
| production method | extracted from mushrooms. 200kg of fresh mushrooms were washed with water, crushed and mixed, and extracted with 1000L boiling water for 8~15h. The resulting suspension is filtered or centrifuged to remove insoluble matter. The aqueous solution is concentrated under reduced pressure to a slight turbidity, and an equal volume of ethanol is added. Filtration or centrifugal separation to collect the precipitate to obtain 320g of components containing lentinan. 50g of the component was suspended in 2L of water and mixed at room temperature to form a uniform viscous brown solution. Then add 20L of water and stir for 1-2h to obtain a clear and uniform solution. Under vigorous stirring, 0.2mol/L CTA-OH aqueous solution (pH 13.2) was added dropwise until pH 7~8 showed flocculent precipitation and a large number of white precipitation appeared at pH 10.5~11.5. 0.2mol/L CTA-OH is added dropwise until no precipitation occurs, and the pH value of the solution is about 12.8. All precipitates were collected by centrifugation and washed with ethanol. It was suspended in 1.2L 20% acetic acid and stirred at 0 ℃ for 5min. The precipitate was collected by filtration, and 1L 50% acetic acid was stirred at 0 ℃ for 3min. The precipitate was collected by centrifugal separation and then dissolved in 2L 6% sodium hydroxide aqueous solution. After centrifugation to remove impurities, add 4L ethanol. Collect the precipitate, wash it twice with ethanol, then wash it once with ether, and dry it in vacuum to obtain powder. The Sevag method was used to unfold with chloroform and 1-butanol, and 3 times the volume of ethanol was added to precipitate it. Collect the precipitate, wash twice with methanol, wash once with ether, and dry in vacuum at room temperature and in the presence of calcium chloride to obtain lentinan. 31g of lentinan can be extracted from 200kg of raw materials. |
Last Update：2024-04-09 21:48:26
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View History
Polysacharid lentinus edodes
1(2H)-Pyridineacetic acid, 2-oxo-
2-thioglycolicacid
606110-99-0
5-({2-nitro-4-methylphenoxy}methyl)-N-(5-methyl-3-isoxazolyl)-2-furamide
1H-pyrazolo[3,4-b]pyridine-3-carboxylic acid
1(2H)-Pyridineacetic acid, 2-oxo-
2-thioglycolicacid
606110-99-0
5-({2-nitro-4-methylphenoxy}methyl)-N-(5-methyl-3-isoxazolyl)-2-furamide
1H-pyrazolo[3,4-b]pyridine-3-carboxylic acid