Name | N,N,N',N'-tetramethyl-p-phenylenediamine dihydrochloride |
Synonyms | N,N,N' TetramethylPPhenylenediamineDihcl Wurster's reagent dihydrochloride TETRAMETHYL-P-PHENYLENEDIAMINE 2 HCL N,N,N',N'-Tetramethylbenzol-1,4-diamindihydrochlorid N,N,N',N'-TETRAMETHYL-P-*PHENYLENEDIAMIN E DIHYDROCH N,N,N',N'-Tetramethyl-p-phenylenediammonium dichloride NNN'N'-Tetramethyl-p-phenylene-diamine dihydrochloride N,N,N',N'-Tetramethyl-P-phenylenediaminedihydrochloride N,N,N',N'-tetramethyl-p-phenylenediamine dihydrochloride N,N,N',N'-Tetramethyl-p-phenylenediamine dihydrochloride N,N,N',N'-Tetramethylbenzene-1,4-diamine dihydrochloride N,N,N',N'-TETRAMETHYL-1,4-BENZENEDIAMINE DIHYDROCHLORIDE N,N,N',N'-TETRAMETHYL-1,4-BENZENEDIAMINE, DIHYDROCHLORIDE N,N,N',N'-TETRAMETHYL-1,4-PHENYLENEDIAMINE DIHYDROCHLORIDE 1,4-Benzenediamine, N,N,N',N'-tetramethyl-, dihydrochloride |
CAS | 637-01-4 |
EINECS | 211-274-8 |
InChI | InChI=1/C10H16N2.2ClH/c1-11(2)9-5-7-10(8-6-9)12(3)4;;/h5-8H,1-4H3;2*1H |
Molecular Formula | C10H18Cl2N2 |
Molar Mass | 237.17 |
Density | 1.1961 (rough estimate) |
Melting Point | 222-224°C(lit.) |
Boling Point | 378.54°C (rough estimate) |
Flash Point | 104.9°C |
Water Solubility | SOLUBLE |
Solubility | Soluble in water, insoluble in ethanol |
Vapor Presure | 0.0121mmHg at 25°C |
Appearance | White-like or gray powder |
Color | off-white to gray |
BRN | 5132096 |
Storage Condition | room temp |
Stability | Stable. Incompatible with strong oxidizing agents, strong bases. |
Sensitive | Light Sensitive |
Refractive Index | 1.6400 (estimate) |
MDL | MFCD00012482 |
Use | This product is for scientific research only and shall not be used for other purposes. |
In vitro study | TMPD (N,N,N′,N′-tetramethyl-para-phenylene-diamine) is used in cell culture and microbiology to differentiate organisms that exhibit cytochrome c oxidase activity and to distinguish between Gram-negative and Gram-positive pathogenic and non-pathogenic bacteria. The concept of TMPD oxidation by microorganisms is widely used and is known as a successful colorimetric indicator for bacterial oxidases, as the radical cation TMPD+˙, formed by oxidation, shows a characteristic deep blue colour. The electrochemical recognition of the TMPD oxidation by bacterial oxidases can be applied to a variety of pathogens. |
Risk Codes | R36/37/38 - Irritating to eyes, respiratory system and skin. R20/21/22 - Harmful by inhalation, in contact with skin and if swallowed. R40 - Limited evidence of a carcinogenic effect |
Safety Description | S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36 - Wear suitable protective clothing. S36/37 - Wear suitable protective clothing and gloves. S22 - Do not breathe dust. S36/37/39 - Wear suitable protective clothing, gloves and eye/face protection. |
UN IDs | 2811 |
WGK Germany | 3 |
FLUKA BRAND F CODES | 8 |
TSCA | Yes |
HS Code | 29215190 |
Hazard Class | 6.1(a) |
Packing Group | II |
EPA chemical substance information | information provided by: ofmpeb.epa.gov (external link) |
Overview | N,N,N',N'-tetramethyl-p-phenylenediamine dihydrochloride, short for TMPD, is an oxidase reagent, can be used to identify Neisseria gonorrhoeae. |
Application | N,N,N',N'-tetramethyl-p-phenylenediamine dihydrochloride, can be used to identify Neisseria gonorrhoeae. The principle is that Neisseria gonorrhoeae has an oxidase, and the oxygen ions it produces can oxidize the oxidase reagent (N,N,N',N'-tetramethyl-p-phenylenediamine dihydrochloride) into a quinone compound, which causes a color reaction. |
biological activity | TMPD dihydrozolide, a compound susceptible to oxidation, it is a redox-active substrate molecule for enzymatic conversion. TMPD dihydrochloride is also an electron donor and acts as a reducing co-substrate for heme peroxidase. TMPD dihydrochloride is also a substrate for complex IV. |