Name | Tetracycline Hydrochloride |
Synonyms | alatet cyclopar artomycin cancycline-250 Tetracycline hcl cefracyclinetablets amycin,hydrochloride chlorowodorkutetracykliny Tetracycline Hydrochloride chlorhydratedetetracycline (2E,6S,12aS)-2-[amino(hydroxy)methylidene]-4-(dimethylamino)-6,10,11,12a-tetrahydroxy-6-methyl-4a,5a,6,12a-tetrahydrotetracene-1,3,12(2H,4H,5H)-trione hydrochloride (2Z,4S,4aS,5aS,6S,12aS)-2-[amino(hydroxy)methylidene]-4-(dimethylamino)-6,10,11,12a-tetrahydroxy-6-methyl-4a,5a,6,12a-tetrahydrotetracene-1,3,12(2H,4H,5H)-trione hydrochloride [4S-(4alpha,4aalpha,5aalpha,6beta,12aalpha)]-4-(Dimethylamino)-1,4,4a,5,5a,6,11,12a-octahydro-3,6,10,12,12a-pentahydroxy-6-methyl-1,11-dioxo-2-naphthacenecarboxamide hydrochloride |
CAS | 64-75-5 1170-81-6 |
EINECS | 200-593-8 |
InChI | InChI=1/C22H24N2O8.ClH/c1-21(31)8-5-4-6-11(25)12(8)16(26)13-9(21)7-10-15(24(2)3)17(27)14(20(23)30)19(29)22(10,32)18(13)28;/h4-6,9-10,15,25,27-28,31-32H,7H2,1-3H3,(H2,23,30);1H |
InChIKey | XMEVHPAGJVLHIG-FMZCEJRJSA-N |
Molecular Formula | C22H25ClN2O8 |
Molar Mass | 480.9 |
Melting Point | 220-223°C(lit.) |
Boling Point | 799.4°C at 760 mmHg |
Specific Rotation(α) | -252 º (c=0.5, H2O) |
Flash Point | 437.3°C |
Water Solubility | 50 g/L |
Solubility | 20g/L water, 5g/L ethanol, insoluble in ether, hydrocarbon and acetone. |
Vapor Presure | 5.82E-27mmHg at 25°C |
Appearance | Yellow crystal |
Color | faint yellow to yellow |
Merck | 14,9196 |
BRN | 3844873 |
PH | pH(10g/l, 25℃) : 1.8~3.0 |
Storage Condition | Keep in dark place,Inert atmosphere,Store in freezer, under -20°C |
Stability | Stable. Incompatible with strong oxidizing agents. |
Sensitive | Air & Light Sensitive |
Refractive Index | -253 ° (C=0.5, 0.1mo |
MDL | MFCD00078142 |
Use | Broad-spectrum antibiotics |
Risk Codes | R36/37/38 - Irritating to eyes, respiratory system and skin. R20/21/22 - Harmful by inhalation, in contact with skin and if swallowed. |
Safety Description | S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36 - Wear suitable protective clothing. |
UN IDs | UN 3077 9 / PGIII |
WGK Germany | 2 |
RTECS | QI9100000 |
FLUKA BRAND F CODES | 8-10-23 |
TSCA | Yes |
HS Code | 29413000 |
Hazard Class | 3 |
Toxicity | LD50 orally in rats: 6443 mg/kg (Goldenthal) |
Reference Show more | 1. Wang Yu, Xu Jia, heading for Su Yu, Zhao Ge, Jiang Jingyan. Effects of three kinds of antimicrobial agents commonly used in veterinary clinic on urease and its mechanism of action by spectroscopic and molecular docking [J]. Journal of Nanjing Agricultural University, 2020,43(04):690-697. 2. Zhou Rui, Gui Rongjie, Zhang Jin, etc. Screening of cadmium and tetracycline contaminated soil tolerant strains and their ability to promote rhizosphere growth. 3. Xue Xiaoxu, Chen Changze, Gu Guowei. Adsorption of drug active compounds on magnetic chitosan composite microspheres modified by polyacrylic acid [J]. Yunnan chemical industry, 2020,47(08):48-51. 4. Wang Shihao, Shi Minghao, Liu Su. Combined toxic effects of tetracycline and arsenic on zebrafish in sequential exposure scenarios [J]. Journal of Environmental Science, 2020,40(12):4590-4597. 5. He Qian, Wang Loli, Yi Chuan, Wu Guiping. Preparation of MoS_2 @ MIL-53(Fe) and its photocatalytic degradation of antibiotics under visible light [J]. Environmental Science & Technology, 2020,43(08):13-19. 6. Xue Xiaoxu, Chen Changze, Gu Guowei. Adsorption of drug active compounds on magnetic chitosan composite microspheres modified by polyacrylic acid [J]. Yunnan chemical industry, 2020,47(08):48-51. 7. [IF = 7.658] Hong Zhang et al."Uncariitannin, a polyphonic polymer from Uncaria gambier, attenuates New York USA through an MgrA-mediated review of alpha-hemolysin." Pharmacol Res. 2019 Sep;147:104328 8. [IF = 7.514] Baoshan He et al."Aptamer-based thin film gold electrode modified with gold nanoparticles and carboxylated multi-walled carbon nanotubes for detecting oxytetracycline in chicken samples."Food Chem. 2019 Dec;300:125179 9. [IF=7.514] Yanan Cao et al."Rapid and selective extraction of norfloxacin from milk using magnetic molecular imprinting polymers nanoparticles."Food Chem. 2021 Aug;353:129464 10. [IF=7.086] Zhifen Chen et al."In-situ synthesis of biochar modified PbMoO4: An efficient visible light-driven photocatalyst for tetracycline removal."Chemosphere. 2021 Dec;284:131260 11. [IF=6.291] Yilin Liu et al."Responses of Hydrocharis dubia (Bl.) Backer and Trapa bispinosa roxb. to tetracycline exposure."Ecotox Environ Safe. 2020 Oct;202:110890 12. [IF=3.209] Liuliu Jiang et al."In vitro and in vivo evaluation of the antifungal activity of fluoxetine combined with antifungals against Candida albicans biofilms and oral candidiasis."Biofouling. 2020;36(5):537-548 13. [IF=2.478] Wang Lina et al."Palygorskite/g-C3N4 conjunction for visible-light-driven degradation of tetracycline hydrochloride."J Mater Sci-Mater El. 2019 Oct;30(19):18159-18167 14. [IF=5.316] Peiyu Cao et al."Constructing nano-heterojunction of MOFs with crystal regrowth for efficient degradation of tetracycline under visible light."J Alloy Compd. 2022 May;904:164061 15. [IF=8.067] Yuanting Xu et al."“1 1>2”: Highly efficient removal of organic pollutants by composite nanofibrous membrane based on the synergistic effect of adsorption and photocatalysis."J Mater Sci Technol. 2022 Oct;124:76 |
(4S,4aS,5aS,6S,12aS)-6-methyl -4-(dimethylamino)-3,6,10,12, 12a-pentahydroxy-1, 11-dioxo-1, 4 ,4a ,5 ,5a ,6 ,11 ,12a-A hydrogen-2-and tetrabenzamide hydrochloride. Tetracycline hydrochloride (C22H24N208 • HCl) shall not be less than 95.0% calculated on a dry basis.
take this product, precision weighing, add 0. The Olmol/L hydrochloric acid solution was dissolved and quantitatively diluted to prepare a solution containing about 10 mg per 1 ml, which was measured according to law (General rule 0621), and the specific rotation was from one 240 ° to one 258 °.
take this product, add water to make a solution containing about 10 mg per lml, and determine it according to law (General 0631). The pH value should be 1.8~2.8.
take 5 parts of this product, 50mg each, respectively, add 5ml of water to dissolve, the solution should be clear; If it is turbid, compare with No. 1 turbidity standard solution (General rule 0902 first method), none should be more concentrated. (For injection)
new system for clinical use. Take this product, plus O.Olmol/L hydrochloric acid solution is dissolved and quantitatively diluted to make a solution containing about 0.8mg per 1 ml as a test solution; 2ml is accurately measured, and 100ml is placed in a measuring flask, and 0.Olmol/L hydrochloric acid solution was diluted to the scale and shaken to serve as a control solution. Take 2ml of the control solution, put it in a 100ml measuring flask, dilute it to the scale with 0.Olmol /L hydrochloric acid solution, and shake it to be used as a sensitivity solution. According to the chromatographic condition test under the content determination item, the sensitivity solution 10u1 is injected into the human liquid chromatograph, and the chromatogram is recorded. The signal-to-noise ratio of the peak height of the principal component chromatogram should be greater than 10. Then the l0ul of the test solution and the control solution are accurately measured and injected into the human liquid chromatograph respectively, and the chromatogram is recorded to 2.5 times of the retention time of the main component peak, if there are impurity peaks in the chromatogram of the test solution, the peak areas of oxytetracycline, 4-episomal tetracycline, chlortetracycline hydrochloride, dehydrated tetracycline and episomal dehydrated tetracycline shall be corrected (multiplied by the correction factor 1.0, respectively), 1.42, 1.39, 0.48 and 0.62) shall not be greater than 0.25 times (0.5% ) , 1.5 times (3.0%), 0.5 times (1.0%), 0.25 times (0.5%) of the main peak area of the control solution, 0.25 times (0.5% ) , and the sum of other impurity peak areas shall not be greater than 0.5 times (1.0%) of the main peak area of the control solution. The peaks in the chromatogram of the test solution which are smaller than the main peak area of the sensitivity solution are ignored.
take this product, at 20~25°C, add 0.8% sodium hydroxide solution to make a solution containing 10 mg per 1 ml, according to UV-visible spectrophotometry (General 0401), put 4cm absorption cell, add 0.8% sodium hydroxide solution from 5 minutes, measured at a wavelength of 530nm, absorbance should not exceed 0.12 (for injection).
take this product, dry to constant weight at 105°C, weight loss should not exceed 1.0% (for oral use) or 0.5% (for injection) (General 0831).
take this product, add sodium chloride injection to make a solution containing 5mg per lml, check according to law (General rule 1142), dose according to rabbit body weight per lkg slow injection of 2ml, should meet the requirements. (For injection)
take this product, dissolve and dilute with appropriate solvent, after membrane filtration treatment, inspection according to law (General rule 1101), should comply with the provisions. (For aseptic dispensing)
measured by high performance liquid chromatography (General 0512).
silica gel bonded with octylsilane as filler; Ammonium acetate solution [0.15mol/L ammonium acetate solution -0.01mol/L ethylene diamine tetraacetic acid disodium solution-triethylamine (100:10:1), with acetic acid to adjust the pH value to 8.5]-acetonitrile (83:17) as mobile phase; Detection wavelength was 280nm. Take about 3mg of the 4-poor tetracycline control, oxytetracycline control, poor dehydrated tetracycline control, chlortetracycline hydrochloride control and dehydrated tetracycline control, and about 48mg of the tetracycline hydrochloride control, and put them in a 100ml measuring flask, add 0.lmol /L hydrochloric acid solution (10ml) was dissolved, diluted to scale with water, and then shaken well. As the applicable solution for the system, 10u1 was injected into the liquid chromatograph, and the chromatogram was recorded: 4-EPI-tetracycline, oxytetracycline, epi-dehydrated tetracycline, tetracycline, chlortetracycline, dehydrated tetracycline, retention time of the tetracycline peak is about 14 minutes. 4-the separation degree between tetracycline peak, oxytetracycline peak, tetracycline peak, tetracycline peak and chlortetracycline peak shall meet the requirements, and the separation degree between chlortetracycline peak and tetracycline peak shall be greater than 1.0.
take this product about 25mg, precision weighing, put 50ml measuring bottle, add 0. Dissolve and dilute the Olmol/L hydrochloric acid solution to the scale, shake well, take 5ml accurately, put it in a 25ml measuring flask, dilute to the scale with 0.01mol/L hydrochloric acid solution, shake well, as a sample solution, the sample was accurately measured by injection of 10u1 into human liquid chromatograph, and the chromatogram was recorded. An appropriate amount of tetracycline hydrochloride reference substance was taken and determined by the same method. According to the external standard method to calculate the peak area, that is.
Tetracycline antibiotics.
shade, seal or seal, and store in a dry place.
This product contains tetracycline hydrochloride (C22H24N208 • HCI) should be 90.0% to 110.0% of the label.
This product is yellow or sugar-coated.
take an appropriate amount of fine powder of this product (about 25mg of tetracycline hydrochloride), Heat 25ml of ethanol, soak it for 20 minutes, then filter it, and let the filtrate dry on a water bath, the same results were obtained according to the tests (1), (2) and (4) for the identification of the residue under the tetracycline hydrochloride.
Take 10 tablets of this product, precision weighing, fine grinding, precision weighing appropriate amount (about 0.25g equivalent to tetracycline hydrochloride), put it in a 250ml measuring flask, add 0.01mol/L hydrochloric acid solution to dissolve and dilute to the scale, shake well, filter, accurately take 5ml of continued filtrate, put it in a 50ml measuring flask, diluted with 0.01mol/L hydrochloric acid solution to the scale, shake, as a test solution, according to the method under the item of tetracycline hydrochloride, obtained.
with tetracycline hydrochloride.
(l)0.125g (2)0.25g
light-shielded, sealed, and stored in a dry place.
This product contains tetracycline hydrochloride (C22H24N208 • HCl) should be 90.0% to 110.0% of the label.
The content of this product was taken, and the same results were shown according to the tests of (1), (2) and (4) under the items of tetracycline hydrochloride.
take the contents under the difference of loading amount, mix evenly, weigh appropriate amount (about 0.25g equivalent to tetracycline hydrochloride) accurately, and add O.Olmol /L hydrochloric acid solution was dissolved and quantitatively prepared to contain about 0.1 mg of the solution was filtered, and the filtrate was taken. As a test solution, it was obtained by measuring according to the method described in the case of tetracycline hydrochloride.
with tetracycline hydrochloride.
0.25g
light-shielded, sealed, and stored in a dry place.
This product is tetracycline hydrochloride plus the right amount of vitamin C or citric acid as a stabilizer of sterile powder. The content of tetracycline hydrochloride (C22H24N208 • HCl) shall be between 93.0% and 107.0% of the labeled amount calculated as the average loading.
This product is yellow mixed with white crystalline powder.
This product was taken and the same results were shown according to the tests of (1), (2) and (4) under the items of tetracycline hydrochloride.
The content under the item of loading amount difference was accurately weighed and an appropriate amount was measured according to the method under the item of tetracycline hydrochloride, and the content was obtained.
with tetracycline hydrochloride.
(1)0.125g (2)0.25g (3)0.5g
It was kept in a dry place and closed.