| Name | Triton X-100 |
| Synonyms | Triton X-770 |
| Physical and Chemical Properties | Tradaton X-100 is a non-ionic surfactant that does not dissociate in water and has high stability in solution. It is not easily affected by strong electrolyte inorganic salts. It can combine with phospholipids and other lipids in biofilms to form a soluble complex; The hydrophobic end can also combine with the hydrophobic area of the membrane protein to form a complex, which is dissolved in the solution. |
Triton X-100 is a non-ionic surfactant commonly used for the extraction, solubilization, lysis, and analysis of proteins or cell membranes. It is also frequently used as a cleaning agent and is ubiquitous in biochemical and biomedical research. Triton X-100 has a chemical structure of a polyethylene glycol ether, possessing strong permeability and hydrophobic properties that effectively disrupt lipid bilayers, making it easier to extract and purify biological macromolecules. As a result, Triton X-100 is widely utilized in biochemical experiments and biological studies.
Properties:
Triton X-100 is a non-ionic surfactant that appears as a white or pale yellow solid, soluble in water and organic solvents. It possesses both hydrophilic and hydrophobic properties, making it useful for disrupting lipid bilayer structures and facilitating the extraction and solubilization of biological macromolecules. It also has high surface activity, aiding in dispersion and stabilization of oils or particles in solution.
Uses:
Triton X-100 is widely used in biochemical and biological research for cell membrane lysis, protein extraction and purification, lipid solubilization, among other applications. Additionally, it is utilized as a cleaning agent and surfactant for removing surface dirt.
Synthesis:
Triton X-100 is typically prepared through chemical synthesis, involving the initial synthesis of ethoxylated phenyl polyethylene glycol, followed by its combination with ethylene oxide to create the final product Triton X-100. The synthesis process is complex and typically carried out under laboratory conditions.
Safety:
Under normal usage, Triton X-100 is generally safe, but it is important to follow safety precautions. Avoid inhalation of its dust or solutions, skin contact, and ingestion. Wear protective gloves and safety goggles when handling. Additionally, Triton X-100 may be toxic to aquatic organisms, so care should be taken when disposing of waste solutions to prevent them from entering water bodies.