| Overview | Donkey serum refers to blood after coagulation, the pale yellow transparent liquid from which fibrin has been separated is removed from the plasma, or the plasma from which fibrin has been removed is used for scientific research, production of diagnostic reagents, and the like. The role of donkey serum is to provide basic nutrients, provide hormones and various growth factors, provide binding proteins, provide pro-contact. Serum should be stored at -5 °c to -20 °c, not more than one month if stored at 4C, if you can not use up a bottle at a time, the sterile sub-package of serum to the appropriate sterile container, put it back in the freezer. |
| Background | Serum, after blood coagulation, removal of the light yellow transparent liquid from which fibrin has been isolated or of the plasma from which fibrin has been removed. Its main role is to provide basic nutrients, provide hormones and various growth factors, provide binding proteins, provide contact-promoting and stretching factors to protect cells from mechanical damage, some protection of cells in culture |
| properties | Donkey serum is a very complex mixture formed by removing fibrinogen from plasma, although most of its components are known, some are still unclear, and the composition and content of serum often vary with the sex, age, physiological conditions and nutritional conditions of the feeding animals. |
| function | serum is a gelatinous liquid that does not contain fibrinogen in plasma, which can maintain the normal viscosity, pH value of blood, osmotic pressure and other effects. It is mainly composed of water and various chemical components, including albumin, alpha 1, Alpha 2, beta, gamma globulin, triglycerides, total cholesterol, alanine aminotransferase and so on. Serum contains various plasma proteins, polypeptides, fats, carbohydrates, growth factors, hormones, inorganic substances, etc., which are physiologically balanced for promoting cell growth or inhibiting growth activity. |
| Storage Conditions | serum that requires long-term storage must be stored in a -20 °c -70 °c freezer. 4 ℃ refrigerator storage time should not exceed 1 month. Because the serum icing volume will increase by about 10%, therefore, the serum in the frozen into the low temperature refrigerator, must reserve a certain volume of space, otherwise prone to pollution or glass bottle cracking. |
| Application | Donkey serum is a large amount of natural medium in cell culture, rich in nutrients necessary for cell growth, often used in in vitro culture of animal cells, has a very important function. Hormones to maintain exponential growth of the cells are provided, with no or little amount of nutrients in the basal medium, and mainly low molecular nutrients. Provides binding proteins that recognize vitamins, lipids, metals, and other hormones, and can bind or modulate the activity of the substances to which they bind. In some cases, the binding protein can combine with the toxic metal and the heat source, and play a role in detoxification. It is the source of factors required for cell attachment and spreading on plastic medium. Function as a pH buffer. Protease inhibitors are provided to inactivate the remaining trypsin during cell passage, protecting the cells from injury. |