Name | bisBenzimide H 33258 H 33258 |
Synonyms | HOECHST 33258 Hoechst 33258 BISBENZIMIDE H 33258 bisBenzimide H 33258 H 33258 hoechst 33258 (bisbenzimide) CELLSTAIN- HOECHST 33258 SOLUTION BISBENZIMIDE TRIHYDROCHLORIDE HYDRATE bisbenzimide trihydrochloride cell*culture tested 4-(5-(4-METHYL-1-PIPERAZINYL)(2,5''-BI-1H-BENZIMIDAZOL)2''-YL)PHENOL p-(5-(5-(4-methylpiperazin-1-yl)benzimidazol-2-yl)benzimidazol-2-yl)phenol 2'-(4-HYDROXYPHENYL)-5-(4-METHYL-1-PIPERAZINYL)-2,5'-BI-1H-BENZIMIDAZOLE TRIHYDROCHLORIDE HYDRATE |
CAS | 23491-44-3 |
EINECS | 245-689-0 |
Molecular Formula | C25H24N6O |
Molar Mass | 424.5 |
Density | 1.355±0.06 g/cm3(Predicted) |
Melting Point | 314 °C |
Boling Point | 746.9±70.0 °C(Predicted) |
Solubility | H2O: 10mg/mL |
Appearance | powder |
pKa | 9.23±0.15(Predicted) |
Storage Condition | −20°C |
Stability | Stable. Incompatible with strong oxidizing agents. Light sensitive. |
Use | Hoechst 33258 is a fluorescent dye used for DNA staining. |
In vitro study | Hoechst 33258, a fluorescent compound with a head-to-tail bis-benzimidazole structure, is initially found to be cytotoxic against L1210 murine leukemia. Hoechst 33258 is evaluated for their cytotoxicity against human tumor cell lines, which are cervix carcinoma cell line (HeLa), Human promyelocytic leukemia cell (HL60) and U937 cell Line. The IC 50 determined in the case of HeLa, HL60 and U937 is 51.31±4.56, 32.43±3.27 and 15.42±2.16 μM for Hoechst 33258, respectively. The cytotoxic property of Hoechst 33258 is investigated on a panel of seven tumour cell lines of different histological origin and Madine-Darby canine kidney (MDCK) normal cells. All cell lines, except MCF-7, exposed to Hoechst 33258 exhibit GI 50 from 84×10 -6 to 191.5×10 -6 mol/dm 3 . Under the same experimental conditions, Hoechst 33258, used as a binder reference compound, stops the cell cycle in S phase and G0/G1. |
Hazard Symbols | Xn - Harmful |
Risk Codes | R22 - Harmful if swallowed R36/38 - Irritating to eyes and skin. |
Safety Description | S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36 - Wear suitable protective clothing. |
WGK Germany | 3 |
RTECS | SM1140500 |
FLUKA BRAND F CODES | 3-8-10 |