estradiol benzoate
estradiol benzoate
CAS: 50-50-0
Molecular Formula: C25H28O3
estradiol benzoate - Names and Identifiers
estradiol benzoate - Physico-chemical Properties
| Molecular Formula | C25H28O3 |
| Molar Mass | 376.5 |
| Density | 1.0493 (rough estimate) |
| Melting Point | 191-198°C(lit.) |
| Boling Point | 465.03°C (rough estimate) |
| Specific Rotation(α) | D25 +58 to +63° (c = 2 in dioxane) |
| Flash Point | 212°C |
| Water Solubility | 0.4mg/L(25 ºC) |
| Solubility | Practically insoluble in water, freely soluble in methylene chloride, sparingly soluble in acetone, slightly soluble in methanol. |
| Vapor Presure | 4.08E-12mmHg at 25°C |
| Appearance | neat |
| Color | White |
| Merck | 3703 |
| BRN | 3107526 |
| pKa | 15.06±0.40(Predicted) |
| Storage Condition | 2-8°C |
| Refractive Index | 1.5460 (estimate) |
| MDL | MFCD00003692 |
| Physical and Chemical Properties | White or yellowish crystalline powder. Odorless. Tasteless. Stable in air. Melting point 191-196 °c. Right optical rotation. Soluble in ethanol, acetone, dioxane, etc., slightly soluble in vegetable oil, insoluble in ether, almost insoluble in water. |
| Use | Can promote the growth and development of female reproductive organs. |
| In vitro study | Estradiol benzoate is a synthetic ester, specifically 3-benzoyl Ester, a natural estrogen, Estradiol. Estrogens guide the development of female genes during embryonic development and puberty. Estradiol is the main female hormone secreted by the ovaries before menopause. Estradiol benzoate is an Estradiol analog that contains a benzyl ester in the C- 3 position and is often used in combination with progesterone to induce estrus in domestic animals. The compound binds to human and murine estrogen receptor α (ERα), as well as to chicken erbium with IC50 values in the range of 22-28 nM. This reflects a 6-10 fold reduction in affinity compared to estradiol. |
estradiol benzoate - Risk and Safety
| Hazard Symbols | T - Toxic |
| Risk Codes | R60 - May impair fertility R61 - May cause harm to the unborn child R40 - Limited evidence of a carcinogenic effect |
| Safety Description | S53 - Avoid exposure - obtain special instructions before use. S22 - Do not breathe dust. S36/37/39 - Wear suitable protective clothing, gloves and eye/face protection. S45 - In case of accident or if you feel unwell, seek medical advice immediately (show the label whenever possible.) |
| WGK Germany | 3 |
| RTECS | KG4050000 |
| FLUKA BRAND F CODES | 8 |
| HS Code | 29372319 |
estradiol benzoate - Reference
| Reference Show more | 1. Fan Zhao, Cao Liming, Liu Qing, et al. Effect of Wenzhong Chushi Quyi decoction on dairy cow placenta retention in cold region in winter [J]. Heilongjiang Journal of Animal Husbandry and Veterinary Medicine, 2020, No.598(10):122-126 160. 2. Luo Wenjing, Liang yuan, Zhou Zhiming, et al. Determination of 20 female hormones in health foods by high performance liquid chromatography-tandem mass spectrometry [J]. Stoichiometry for chemical analysis, 2019(3). |
estradiol benzoate - Standard
Authoritative Data Verified Data
This product is 3-hydroxyestrane-1, 3,5 (10)-triene-17 tablets of alcohol -3-benzoate, calculated as dry product, the content of C25H2803 shall be 97.0% ~ 103.0%.
Last Update：2024-01-02 23:10:35
estradiol benzoate - Trait
Authoritative Data Verified Data
- This product is white crystalline powder; Odorless.
- This product is slightly soluble in acetone, slightly soluble in ethanol or vegetable oil, insoluble in water.
melting point
The melting point of this product (General 0612) is 191 ~ l96 °C.
specific rotation
take this product, precision weighing, and two oxygen six ring dissolved and quantitative dilution made of each lml containing 10 mg of the solution, according to the law (General 0621), the specific rotation was 58 ° to 63 °.
absorption coefficient
take this product, precision weighing, plus absolute ethanol dissolution and quantitative dilution of 10ug per lml solution, according to UV-visible spectrophotometry (General 0401), the absorbance was measured at a wavelength of 230nm, and the absorption coefficient was 490 to 520.
Last Update：2022-01-01 13:33:53
estradiol benzoate - Differential diagnosis
Authoritative Data Verified Data
- take about 2mg of this product, add 2ml sulfuric acid to dissolve, the solution is yellow-green, and blue fluorescence; Pour this solution into 2ml of human water, the solution is light orange.
- in the chromatogram recorded under the content determination item, the retention time of the main peak of the test solution should be consistent with the retention time of the main peak of the reference solution.
- The infrared absorption spectrum of this product should be consistent with that of the control (Spectrum set 235).
Last Update：2022-01-01 13:33:54
estradiol benzoate - Exam
Authoritative Data Verified Data
Related substances
take this product, add acetonitrile to dissolve and dilute to make a solution containing 2mg per 1ml as a test solution; Take 1ml for precision measurement and put it in a 100ml measuring flask, as a control solution, it was diluted to the scale with acetonitrile and shaken. According to the high performance liquid chromatography (General 0512) test, with eighteen alkyl silane bonded silica gel as filler, acetonitrile-water (60:40) as mobile phase A, acetonitrile as mobile Phase B, the linear gradient elution was performed according to the following procedure, and the flow rate was adjusted so that the retention time of the Estradiol benzoate peak was approximately 26 minutes and the detection wavelength was 230mn. The number of theoretical plates shall not be less than 4500 calculated by the peak of Estradiol benzoate, and the separation degree between the peak of Estradiol benzoate and the adjacent impurity peaks shall meet the requirements. l0ul of the test solution and the control solution were respectively injected into the liquid chromatograph, and the chromatograms were recorded. If there are impurity peaks in the chromatogram of the test solution, the area of a single impurity peak shall not be greater than the area of the main peak of the control solution (1.0%), and the sum of each impurity peak area shall not be greater than 1.5 times (1.5%) of the area of the main peak of the control solution.. The chromatogram of the test solution is 0.01 times smaller than the main peak area of the control solution.
loss on drying
The wood was dried to constant weight at 105°C, and the weight loss should not exceed 0.5% (General rule 0831).
Last Update：2022-01-01 13:33:54
estradiol benzoate - Estradiol benzoate injection
Authoritative Data Verified Data
This product is a sterile oil solution of Estradiol benzoate. Estradiol urinates (C25H2803) shall be present at 90.0% to 110.0% of the labeled amount.
trait
This product is a light yellow clear oily liquid.
identification
- (1) take an appropriate amount of this product (about 1 mg equivalent to Estradiol benzoate), add 10ml of anhydrous ethanol, shake strongly, place it in an ice bath to make layers, and take the upper layer of ethanol solution, in the centrifuge tube, centrifuge, take the h clear solution as the test solution; Take the Estradiol benzoate control substance, dissolve and dilute with anhydrous ethanol to make 0.1 mg solution, as a control solution. According to the thin layer chromatography (General 0502) test, absorb the above two solutions 10ul, respectively, on the same silica gel G thin layer plate, with benzene-ether-glacial acetic acid (50:30:0.5) for the purpose of developing the solvent, spreading, drying, spraying with sulfuric acid-anhydrous ethanol (1:1), heating at 105 ° C. For 10-20 minutes, taking out, cooling, and placing under a UV lamp (365nm) for inspection. The position and color of the main spot displayed by the test solution should be the same as that of the reference solution.
- ( 2) in the chromatogram recorded under the content determination item, the retention time of the main peak of the test solution should be consistent with the retention time of the main peak of the reference solution.
- two items (1) and (2) above can be selected as one item.
examination
- the relevant substances should be accurately measured with a volume pipette. Take an appropriate amount of this product (about 2mg equivalent to Estradiol benzoate), put it in a 100ml measuring flask, add an appropriate amount of anhydrous ethanol, and shake it thoroughly. After the solution is clarified, dilute with absolute ethanol to the scale, shake, as a test solution; Precision take 1ml, 100ml flask, diluted with absolute ethanol to the scale, shake, as a control solution. According to the chromatographic conditions under the item of related substances of Estradiol benzoate, 20 u1 of each of the test solution and the control solution are accurately measured, and human liquid chromatography is injected respectively to record the chromatogram. If there are impurity peaks in the chromatogram of the test solution, the area of a single impurity peak shall not be greater than the area of the main peak of the control solution (1.0% ) , and the sum of the areas of each impurity peak shall not be greater than 1 of the area of the main peak of the control solution. Times (1.5%). The chromatogram of the test solution is 0.01 times smaller than the main peak area of the control solution.
- others should comply with the relevant provisions under injection (General 0102).
Content determination
take an appropriate amount of this product (about 2mg equivalent to Estradiol benzoate) with an internal volume pipette, put it in a 100ml measuring flask, add an appropriate amount of anhydrous ethanol, fully shake, and after the solution is clarified, dilute to the scale with absolute ethanol, shake, as a test solution, according to the chromatographic conditions under the item of Estradiol benzoate content determination, take 20u1 injection of human liquid chromatograph with precision, record the chromatogram; in addition, an appropriate amount of Estradiol benzoate reference substance was accurately weighed, dissolved and quantitatively diluted with absolute ethanol to make a solution containing about 20ug per 1 ml, and the same method was used for determination. According to the external standard method to calculate the peak area, that is.
category
with Estradiol benzoate.
specification
(l ) lml:lmg (2)lml:2mg (3)lml:5mg
storage
light shielding, closed storage.
Last Update：2022-01-01 13:33:56
estradiol benzoate - Storage
light shielding, sealed storage.
Last Update：2024-04-10 22:29:15
estradiol benzoate - Content determination
measured by high performance liquid chromatography (General 0512).
chromatographic conditions and system suitability test
silica gel bonded with eighteen alkyl silane was used as a filler; Methanol-water (80:20) was used as a mobile phase; The detection wavelength was 230mn. The number of theoretical plates is not less than 4500 calculated from the peak of Estradiol benzoate.
assay
take about 25mg of this product, precision weighing, put it in 25ml measuring flask, add methanol to dissolve, cool, dilute to the scale with methanol, shake, take 5ml precision, place in 25ml measuring flask, dilute to the scale with methanol, shake well, as a test solution, take 10u1 injection of human liquid chromatography with precision, record the chromatogram; Take Estradiol benzoate reference substance, and determine with the same method. According to the external standard method to calculate the peak area, that is.
Last Update：2024-04-09 21:01:54
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View History
estradiol benzoate
Pterocarpadiol A
134419-57-1
Glycerol Tristearate
1,4-diisocyanato-Benzene
4-(3-Amino-1H-1,2,4-triazol-5-yl)-1-methylpyrrolidin-2-one
17α-Hydroxyyohimban-16α-methanol 16,17-sulfurous acid
3-(Phenylsulfonyl)cyclohexene
80155-81-3
1,3-dibromoacetone
Pterocarpadiol A
134419-57-1
Glycerol Tristearate
1,4-diisocyanato-Benzene
4-(3-Amino-1H-1,2,4-triazol-5-yl)-1-methylpyrrolidin-2-one
17α-Hydroxyyohimban-16α-methanol 16,17-sulfurous acid
3-(Phenylsulfonyl)cyclohexene
80155-81-3
1,3-dibromoacetone