Tetraiodofluorescein (TIF) is an organic dye with the molecular formula C22H18I4O5. It is a blue red crystalline powder with poor solubility in water, but good solubility in alcohol solvents. Tetraiodofluorescein has strong fluorescence properties and can emit blue-green fluorescence when exposed to ultraviolet light. The application fields of tetraiodofluorescein are very extensive, mainly including the following aspects: Biological experiments: Tetraiodofluorescein is commonly used in biological experiments as a fluorescent probe for labeling and detecting proteins, cells, nucleic acids, etc. By observing under a fluorescence microscope, the structure and dynamic changes of cells and organelles can be clearly observed, providing convenience for biological research. Medical diagnosis: Tetraiodofluorescein is also widely used in the medical field, such as for pathogen detection and disease diagnosis. For example, when detecting diseases such as tuberculosis and dysentery, the morphology and distribution of bacteria can be observed through tetraiodofluorescein staining, providing a basis for clinical diagnosis. Chemical analysis: Tetraiodofluorescein can also be used as a tracer for chemicals. For example, in fields such as petrochemicals and environmental monitoring, compounds can be labeled with tetraiodofluorescein to facilitate detection and analysis of target substances. Materials Science: Tetraiodofluorescein can also be applied in materials science, such as preparing composite materials with fluorescent properties, polymer materials, etc. In addition, it also plays an important role in the research of nanomaterials. Other fields: Tetraiodofluorescein is also used in fields such as agriculture and food science. For example, fluorescent labeling can be used as a plant growth regulator in agriculture to facilitate the study of plant growth processes
In the food industry, it can be used to detect harmful substances in food. In summary, tetraiodofluorescein, as a widely used fluorescent dye, plays an important role in multiple fields. However, during use, please pay attention to following safety regulations, ensuring a good ventilation environment, and taking appropriate protective measures.