DRAQ5 MedChemExpress (MCE)

Product Name	DRAQ5
Synonyms	DRAQ5;9,10-Anthracenedione, 1,5-dihydroxy-4,8-bis[[2-(methylamino)ethyl]amino]-
CAS	254098-36-7
EINECS
Chemical Formula	C20 H24 N4 O4
Molecular Weight	384.43
inchi
Package	20 μL;50 μL
Price	Email to quote
Descriptions	DRAQ5 DRAQ5 MedChemExpress (MCE) HY-D1742 254098-36-7 4°C, protect from light In solvent : -80°C, 6 months Descriptions DRAQ5 DRAQ5 MedChemExpress (MCE) HY-D1742 254098-36-7 4°C, protect from light In solvent : -80°C, 6 months -20°C, 1 month (protect from light) Room temperature in continental US may vary elsewhere. DRAQ5 is a novel cell permeant and far red-fluorescing DNA probe. DRAQ5 excites at a wavelength of 647 nm, close to the Ex, and produces a fluorescence spectrum extending from 665 nm out to beyond 780 nm wavelengths. DRAQ5 fluorescence reflects cellular DNA content. DRAQ5 can be used in combination with FITC and RPE-labelled antibodies, without the need for fluorescence compensation. Mammalian cell in full culture medium staining methods[2]: (1) Cell planking: Digestive separation of cells and resuspend in complete medium to a concentration of 2-4 × 105 cells/ml. Note: Attached cell cultures (e.g., coverslip cultures or chambered wells) can be stained in a 1-2-ml staining volume overlayering a 4-cm2 surface area. (2) Prepare staining solution: Add 4 µl of 5 mM DRAQ5 acidified stock per ml culture medium (20 µM final). Note: Nuclear discrimination is achievable at 2.5 to 5 µM, and it is unlikely that concentrations >30 µM would be required. (3) Fluorescence staining: Incubate 5 to 15 min at 37°C. Note: Overstaining cannot occur. (4)Wash (optional): Centrifuge cells 3 to 5 min at 800 × g, 37°C. Discard supernatant and resuspend in complete medium with 10 mM HEPES (HY-D0857) at 4 × 105 cells/ml. (5) For flow cytometry: Use conventional pulse analysis for doublet discrimination and analyze parameters using appropriate software. (6) For laser scanning microscopy: Collect fluorescence images using a 695 nm long-pass filter. Fixed cells staining methods[2]: (1) Fixed cells:Use 4% paraformaldehyde in PBS for 30 min with resuspension in an aqueous buffer (e.g., PBS). (2) Fluorescence staining: similar concentrations of dye and similar incubation conditions can be used as for live cells. \| \| \| \| \| \| \| \| \| \| [1]. Smith PJ, et al. A novel cell permeant and far red-fluorescing DNA probe, DRAQ5, for blood cell discrimination by flow cytometry. J Immunol Methods. 1999 Oct 29 229(1-2):131-9. [Content Brief] [2]. Smith PJ, et al. DRAQ5 labeling of nuclear DNA in live and fixed cells. Curr Protoc Cytom. 2004 May Chapter 7:Unit 7.25. [Content Brief]
Last Update	2025-04-01 14:45:47

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