Suc-AAPF-pNA MedChemExpress (MCE)

Product Name	Cathepsin G Substrate
Synonyms	Cathepsin G Substrate SUC-ALA-ALA-PRO-PHE-PNA Cathepsin G Substrate I N-SUCCINYL-ALA-ALA-PRO-PHE-PNA N-SUCCINYL-ALA-ALA-PRO-PHE P-NITROANILIDE N-Succinyl-Ala-Ala-Pro-Phe 4-nitroanilide Synonyms Cathepsin G Substrate SUC-ALA-ALA-PRO-PHE-PNA Cathepsin G Substrate I N-SUCCINYL-ALA-ALA-PRO-PHE-PNA N-SUCCINYL-ALA-ALA-PRO-PHE P-NITROANILIDE N-Succinyl-Ala-Ala-Pro-Phe 4-nitroanilide succinyl-alanyl-alanyl-prolyl-phenylalanine-4-nitroanilide N-succinyl-alanyl-alanyl-prolyl-phenylanaline nitroanilide N-SUCCINYL-L-ALANYL-L-ALANYL-L-PROLYL-L-PHENYLALANINE 4-NITROANILIDE N-Succinyl-Ala-Ala-Pro-Phe-pNA, N-Succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine 4-nitroanilide
CAS	70967-97-4
EINECS
Chemical Formula	C30H36N6O9
Molecular Weight	624.64
inchi
Package	5 mg;10 mg;25 mg
Price	Email to quote
Descriptions	Suc-AAPF-pNA Suc-AAPF-pNA MedChemExpress (MCE) HY-P2573 70967-97-4 Suc-Ala-Ala-Pro-Phe-pNA 99.87% Sealed storage, away from moisture and light, under nitrogen Powder -80°C 2 years -20°C 1 year In solvent : -80°C, 6 months Descriptions Suc-AAPF-pNA Suc-AAPF-pNA MedChemExpress (MCE) HY-P2573 70967-97-4 Suc-Ala-Ala-Pro-Phe-pNA 99.87% Sealed storage, away from moisture and light, under nitrogen Powder -80°C 2 years -20°C 1 year In solvent : -80°C, 6 months -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen) Room temperature in continental US may vary elsewhere. Suc-AAPF-pNA (Suc-Ala-Ala-Pro-Phe-pNA) is a chromogenic p-nitroanilide (pNA) substrate with the Km of 1.7 mM. Cleavage of Suc-AAPF-pNA releases 4-nitroaniline, which is yellow in colour and can be measured spectrophotometrically. Suc-AAPF-pNA can be used for the measurement of free and membrane-bound cathepsin G in human neutrophils. The elastase activity is measured as activity against the substrate Suc-AAPF-Pna. Aliquots (10 μL) of SmCTF samples are incubated at 30 °C with 200 μM Suc-AAPF-pNA and the activity of cercarial elastase measured as abosorbance at 400 nm[2]. Proteolytic activity is measured by hydrolysis of the substrate suc-AAPF-pNA. Serial dilutions of the proteases are made in 100 mM Tris buffer, pH 8.6, containing 10 mM CaCl2. Enzyme activity is measured at 25°C in 100 mM Tris/HCl, pH 8.6 containing 1.6 mM suc-AAPF-pNA, 0.0005% Tween-80 and 1% DMSO by monitoring the absorbance change at 410 nm using a spectrophotometer. Each dilution of enzyme is tested in triplicate[3]. {Suc}-Ala-Ala-Pro-Phe-{pNA} \| \| \| \| \| \| \| \| \| \| [1]. Sylvie Attucci, et al. Measurement of free and membrane-bound cathepsin G in human neutrophils using new sensitive fluorogenic substrates. Biochem J. 2002 Sep 15 366(Pt 3):965-70. [Content Brief] [2]. A J Webb, et al. A protease-based biosensor for the detection of schistosome cercariae. Sci Rep. 2016 Apr 19:6:24725. [Content Brief] [3]. Jeanette M Mucha, et al. Enhanced immunogenicity of a functional enzyme by T cell epitope modification. BMC Immunol. 2002:3:2. [Content Brief]
Last Update	2025-04-01 14:45:47

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