Ac-VEID-AMC MedChemExpress (MCE)

Product Name	AC-VEID-AMC
Synonyms	AC-VEID-MCA AC-VEID-AMC AC-VAL-GLU-ILE-ASP-7-AMINO-4-METHYLCOUMARIN N-ACETYL-VAL-GLU-ILE-ASP-7-AMINO-4-METHYLCOUMARIN AMC ACETYL-L-VALYL-L-GLUTAMYL-L-ISOLEUCYL-L-ASPARTIC ACID ALPHA-(4-METHYLCOUMARYL-7-AMIDE)
CAS	219137-97-0
EINECS
Chemical Formula	C32H43N5O11
Molecular Weight	673.71
inchi
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Descriptions	Ac-VEID-AMC Ac-VEID-AMC MedChemExpress (MCE) HY-P2065 219137-97-0 Please store the product under the recommended conditions in the Certificate of Analysis. Room temperature in continental US Descriptions Ac-VEID-AMC Ac-VEID-AMC MedChemExpress (MCE) HY-P2065 219137-97-0 Please store the product under the recommended conditions in the Certificate of Analysis. Room temperature in continental US may vary elsewhere. Ac-VEID-AMC is a fluorescent substrate for the determination of caspase-6 and related cysteine protease activities (Ex=340-360 nm, Em=440-460 nm). Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Enzyme activity (rabbit osteoclasts for example)[1]: 1. Treat purified, differentiated cells by medium for 48 h (time depends on the cell). 2. Put treated cells into 4.5 mL lysis buffer. 3. Each lysate is divided into thirds, then incubate with 40 μM Ac-VEID-AMC at 37°C for 1 h. 4. Detect cleavage of fluorescent substrates (Ex=340-360 nm, Em=440-460 nm) 460 380 \| \| \| \| \| \| \| \| \| \| [1]. Benford HL, et al. Visualization of bisphosphonate-induced caspase-3 activity in apoptotic osteoclasts in vitro. Bone. 2001 May 28(5):465-73. [Content Brief]
Last Update	2025-04-01 14:45:47

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