N1-Methylpseudouridine MedChemExpress (MCE)

Product Name	1-methylpseudouridine
Synonyms	U-50228 Antibiotic U-50228 1-N-ME-PSEUDOURIDINE 1-methylpseudouridine N1-Methylpseudouridine 1-Methyl-5-(β-D-ribofuranosyl)-2,4(1H,3H)-pyrimidinedione 1-Methyl-5-(β-D-ribofuranosyl)pyrimidine-2,4(1H,3H)-dione Synonyms U-50228 Antibiotic U-50228 1-N-ME-PSEUDOURIDINE 1-methylpseudouridine N1-Methylpseudouridine 1-Methyl-5-(β-D-ribofuranosyl)-2,4(1H,3H)-pyrimidinedione 1-Methyl-5-(β-D-ribofuranosyl)pyrimidine-2,4(1H,3H)-dione (-)-1-Methyl-5-(β-D-ribofuranosyl)-2,4(1H,3H)-pyrimidinedione
CAS	13860-38-3
EINECS
Chemical Formula	C10H14N2O6
Molecular Weight	258.23
inchi
Package	10 mM * 1 mL;5 mg;10 mg;25 mg;50 mg;100 mg
Price	Get quote
Descriptions	N1-Methylpseudouridine N1-Methylpseudouridine MedChemExpress (MCE) HY-112582 13860-38-3 1-Methylpseudouridine Descriptions N1-Methylpseudouridine N1-Methylpseudouridine MedChemExpress (MCE) HY-112582 13860-38-3 1-Methylpseudouridine N1-methyl-pseudouridine 99.95% Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year Room temperature in continental US may vary elsewhere. N1-methyl-pseudouridine (1-Methylpseudouridine), a methylpseudouridine, outperforms 5 mC and 5 mC/N1-methyl-pseudouridine in translation. N1-methyl-pseudouridine in mRNA enhances translation through eIF2α-dependent and independent mechanisms by increasing ribosome density. Incorporation of N1-methyl-pseudouridine into mRNA modifies mRNAs produced higher amounts of luc than the standard Luc mRNA in HEK293T cells. Incorporation of N1-methyl-pseudouridine nucleoside modification in both Luc and GFP mRNA enhances the initiation step of translation, in part by suppressing eIF2α phosphorylation. In addition, polysome formation and growth on the NN1-methyl-pseudouridine-containing Luc mRNA is enhanced due to the reduction of elongation rate.? In all the in vitro translation systems, incorporation of N1-methyl-pseudouridine in Luc and GFP mRNAs dramatically enhanced translation. The N1-methyl-pseudouridine-Luc mRNA is associated with heavier polysomes than Luc mRNA[1]. N1-methylpseudouridine-incorporated mRNA outperforms pseudouridine-incorporated mRNA by providing enhanced protein expression and reduced immunogenicity in mammalian cell lines and mice[2].?N1-methyl-pseudouridine (1-Methylpseudouridine) (20 μg I.m. or i.d. routes for 21 days) and m5C/ N1-methyl-pseudouridine-modified mRNA respectively have a higher translational capacity than Ψ and m5C/Ψ-modified mRNA in vivo[2]. \| \| \| \| \| \| \| \| \| \| [1]. Svitkin YV, et al. N1-methyl-pseudouridine in mRNA enhances translation through eIF2α-dependent and independent mechanisms by increasing ribosome density. Nucleic Acids Res. 2017 Jun 2 45(10):6023-6036. [Content Brief] [2]. Andries O, et al. N(1)-methylpseudouridine-incorporated mRNA outperforms pseudouridine-incorporated mRNA by providing enhanced protein expression and reduced immunogenicity in mammalian cell lines and mice. J Control Release. 2015 Nov 10 217:337-44. [Content Brief] [3]. Nance KD, Meier JL. Modifications in an Emergency: The Role of N1-Methylpseudouridine in COVID-19 Vaccines. ACS Cent Sci. 2021 7(5):748-756. [Content Brief]
Last Update	2025-04-01 14:45:47

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