| Molecular Formula | C8H4N2O4 |
| Use | For organic synthesis and used as pigments, pharmaceutical intermediates |
Place in a cool, dry place away from light and high temperature.
1. Antibacterial and enhanced immune function: the test showed that honeysuckle on typhoid Bacillus, paratyphoid Bacillus, Escherichia coli, Proteus, Pseudomonas aeruginosa, pertussis Bacillus, Vibrio cholerae, Staphylococcus, Streptococcus, pneumococcus, meningococci, etc. have inhibitory effect.
2, inhibition of drug-resistant bacteria protein synthesis: honeysuckle extract on Staphylococcus aureus drug-resistant plants have a significant stimulating effect, more for drug-resistant strains of internal medicine and surgery caused by inflammation, such as the treatment of pulmonary tuberculosis complicated with respiratory tract infection, pneumonia, acute bacillary dysentery, diarrhea. It is also used to reduce the rate of throat bacteria.
| Plant Source: | honeysuckle |
| plant extract | honeysuckle is a common traditional Chinese medicine in China, which is the dried flower bud of Lonicera japonica Thunb. Honeysuckle extract obtained after extraction and drying has been widely used in yinhuang Oral Liquid, yinhuang capsule, yinhuang granules and other proprietary Chinese medicines. Chlorogenic acid, the main active ingredient in honeysuckle extract, has the pharmacological effects of anti-virus, improving immunity and lowering blood lipid. Honeysuckle extract in the pharmaceutical, spices, cosmetics, health food, health drinks and other fields gradually should be, the prospect is very broad. |
| extracted from | honeysuckle is the dried flower bud or just-open flower of Lonicera japonica Thunb. Also known as Lonicera japonica flower, quails flower, silver flower, double flowers, two flowers, golden vine flower, double bud flower, Golden Flower, two flower Flower, leaf Vine, Tea Flower. In the altitude of 1500m in the Hill, Valley, forest, side hedges, wild or cultivated, often round ornamental shade plants. It is distributed in most parts of China. Mainly produced in Henan and Shandong. Henan production is called "South Yinhua", Shandong production is called "East Yinhua" or "Ji Yinhua". Climbing shrubs, 3-6 m in length. Young branches were densely pilose and glandular trichomes. Leaves opposite, with short sessile; Leaf blade broadly-linear or elliptic, 3-8cm long, 2-4cm wide, apex acuminate, base rounded, entire. Flowers paired in leaf axillae; Bracts 1 pair, leaf-like, bracteoles free; Calyx tube hairless, calyx teeth 5, apiculate and ciliated; Corona tube-like, 3-4cm long, first white, then light yellow, aromatic, lipped, upper lip 4-lobed upright, lower lip inverted; Stamens 5. Berries spherical, black. Flowering period May -8 months, fruiting period 8-9 months. |
| Chemical composition | 1. The main effective component of the organic acid honeysuckle extract is chlorogenic acid compound. There are mainly chlorogenic acid (Chlorogcnic acid) and isochlorogenic acid (isochlorogenic acid). Chlorogenic acid (CGA), also known as coffee tannic acid, is a condensed phenolic acid formed by caffeic acid and quinic acid, belonging to phenylpropanoid compounds. Isochlorogenic acid as a mixture, its isomers were 4, 5-dicaffeic acid quinic acid, 3, 4-dicaffeic acid quinic acid, 3, 5-dicaffeic acid quinic acid, 1, 3-dicaffeic acid acylquinic acid, 3-feruloylquinic acid, 4-feruloylquinic acid, 5-feruloylquinic acid. The first flavonoid extracted from Flos lonicerae is luteolin, followed by Lonicera glycoside. Four flavonoids, luteolin-3-o-α-d-glucoside, luteolin-7-O-β-D galactoside, quercetin-7-o-β-d-glucoside and hyperin, were isolated from n-butanol extract of Lonicera japonica Thunb. 5-hydroxy-3 ',4', 7-trimethoxyflavone and Corymbosin were separately obtained from the chloroform extract of honeysuckle. 3. The volatile oil has the flower alcohol, linalool. The two accounted for 49.4% of the total volatile oil. It also contains eugenol, geraniol, α-terpineol and so on, and 47 compounds have been identified. Other still containing inositol, saponin, tannic acid, etc. Chlorogenic acid: molecular formula C16H18O9, molecular weight 354.30. The hemihydrate was a needle-like crystal (water) which became an anhydrous compound at 110 ° C., MP. 208 ° C.,[α]D26-35.2 °(C = 2.8). 25 ° C water solubility of 4%, hot water solubility greater, soluble in ethanol and acetone, very slightly soluble in ethyl acetate. Honeysuckle leaves contain Loniceraflavone (Loniceraflavone): Molecular formula C15H10O5, Lonicein (Lonicein), Loganin (Loganin) and tannin. |
| pharmacological action | 1. Anti-inflammatory, antipyretic and anti-allergic effects honeysuckle extract can inhibit carrageenan-induced foot swelling in rats and reduce the degree of egg white-induced foot swelling. It also has obvious anti-exudative and anti-proliferative effects on Croton oily granulation capsule in rats. Honeysuckle has obvious antipyretic effect on experimental animal Fever model. Yinqiao powder has a significant inhibitory effect on a variety of experimental inflammation, especially allergic inflammation, and can obviously inhibit Fever of rats induced by 2, 4-Dinitrophenol. 2. Anti-Fertility Effect of honeysuckle ethanol extract decoction after non-oral administration has obvious anti-fertility effect, intraperitoneal injection of mice early, middle and late pregnancy are effective, intravenous drip or intrauterine administration is also effective for early pregnancy in rabbits. Honeysuckle can make early pregnancy rat plasma progesterone decreased significantly, the anti-early pregnancy effect of honeysuckle can be exogenous progesterone, human chorionic gonadotropin (HCG) completely canceled, flos lonicerae can also significantly inhibit the formation of decidual tumor in pseudopregnant mice, and this effect can also be antagonized by progesterone. 3. Antioxidant effect the aqueous extract of Lonicera japonica has a direct elimination effect on hydrogen peroxide (H2O2) in vitro and a linear dose-effect relationship, but whether it has a role in vivo remains to be further studied. In addition, honeysuckle can improve the release of H2O2 from neutrophils in scalded mice to a certain extent, and can reduce the ability of neutrophils to synthesize and release lysosomal enzymes in scalded mice, indicating that it has the effect of antioxidant response. The results showed that the crude extract of chlorogenic acid and the crude flavonoids had significant inhibitory effect on the peroxidation of oil. Honeysuckle glycosides and chlorogenic acid are two kinds of phenolic acid compounds with high content in Lonicera japonica leaves. Phenolic acid is a kind of typical compounds with free radical scavenging effect, and honeysuckle glycosides is slightly stronger than chlorogenic acid. The antioxidant activity of Flos lonicerae was studied by DPPH method, and the antioxidant activity was expressed by inhibition rate. When the concentration of Flos lonicerae is 5 mg/mL, the inhibition rate is 95.4%, and when the extract is diluted 5 times, the concentration is 1 mg/ml, the inhibition rate is 94.3%, which indicates that Flos lonicerae has strong antioxidant ability. 4. The chlorogenic acid contained in the extract of liver and gallbladder honeysuckle has beneficial biliary effect and can enhance the secretion of bile in rats. Hypodermic injection of 200mg/kg of total saponins from Lonicera fulvotomentosa can significantly antagonize the increase of GPT in liver and the content of triglyceride in liver induced by CCl4, paracetamol and galactosamine, and obviously reduce the severity of liver pathological injury, the sum of the number of liver punctate necrosis and the occurrence rate of necrosis were significantly reduced. Hypolipidemic, enhance immune function rats intragastric 2.5g/kg honeysuckle extract, can reduce intestinal cholesterol absorption, lower plasma cholesterol levels. In vitro tests also found that honeysuckle can be combined with cholesterol. Total saponins of Lonicera fulvotomentosa can significantly reduce the content of liver triglyceride in normal mice, and the content of liver triglyceride in mice poisoned by CCl4, paracetamol and galactosamine. Flos lonicerae aqueous extract can promote the phagocytic function of peripheral blood leukocytes. Intraperitoneal injection of Flos lonicerae injection could also promote the phagocytosis of inflammatory cells. 250mg/kg aqueous extract of Flos lonicerae can reduce the percentage of ANAE positive T cells, suggesting that it may have inhibitory effect on cellular immunity. In addition, chlorogenic acid has the function of increasing white blood cells as the main active ingredient of ramie. 6. Hemostatic effect chlorogenic acid and caffeic acid contained in honeysuckle extract have significant hemostatic effect, which can shorten the coagulation time and bleeding time. Chlorogenic acid and caffeic acid are also effective hemostatic components of ramie root. Chlorogenic acid is not resistant to high temperature, long time heating will destroy and reduce the efficacy. Traditional hemostatic silver flower charcoal, may be related to this. Central excitatory action oral chlorogenic acid can cause rats, mice and other animals in the central nervous system excited, the intensity of caffeine 1/6, the combination of the two no additive and enhance the role. 8. Antibacterial, antiviral effect honeysuckle extract antibacterial range is wide, the Staphylococcus aureus, hemolytic Streptococcus, Pneumonia Diplococcus, Bordetella pertussis and other Gram-positive bacteria have inhibitory effect, and Shigella dysenteriae, Salmonella Typhi, paratyphoid bacilli, Vibrio cholerae, Pseudomonas aeruginosa, Escherichia coli, human tuberculosis, Meningococcus and other Gram-negative bacteria also have a strong inhibitory effect on Leptospira is also effective. The water decoction of this product has in vitro inhibitory effect on a variety of dermatophytes, such as Trichophyton panularis, xanthophyton Xu, Trichophyton Rustum, epidermal ringworm, and so on. Recent studies have shown that honeysuckle has antibacterial effect on the common pathogenic bacteria of oral diseases, such as Streptococcus mutans, Actinobacillus, gingivalis, and melanin producing Bacillus. Honeysuckle or its compound, on the respiratory tract Virus such as influenza Asia A, orphan Virus has inhibitory effect, and can inhibit and delay the cytopathic effect. In addition, the herpes Virus also has a certain inhibitory effect. It is generally believed that the main active ingredient is a chlorogenic acid compound. Other effects in vitro the water and alcohol extracts of Flos lonicerae have obvious cytotoxic effects on sarcoma S180 and Ehrlich ascites carcinoma in mice. Oral administration of honeysuckle extract has mild preventive effect on pylorus ligated gastric ulcer in rats. Oral administration of large doses of chlorogenic acid can enhance gastrointestinal peristalsis, promote gastric juice and bile secretion. Chlorogenic acid and its decomposition products have Excitatory effect on isolated rat uterus. Chlorogenic acid contained in Flos lonicerae can stimulate the central nervous system of rats and mice. In addition, chlorogenic acid can slightly enhance the pressor effect of epinephrine and norepinephrine on cats and rats, but has no effect on the instantaneous membrane reaction of cats. |
| extraction method | 1.1kg of honeysuckle was extracted by sulfur method, and then decocted with water for 2H each time. The combined decoction was filtered, and the filtrate was adjusted to pH 10~12 by adding lime milk, adjust the pH value with sulfuric acid to 6~7, stir, filter, filtrate is concentrated to thick paste, spray drying, then get honeysuckle extract. 2.1kg of honeysuckle was taken by modified sulfur method, and (15, 10) times the amount of water, extracted 2 times at 80~90 ℃, 2h each time, filtered, combined with filtrate, after the filtrate is concentrated to 2 /5 of the original volume, add 20% lime milk to adjust the pH value to 10~12, stand for 12h, filter out the precipitate, add 2 times the amount of 95% ethanol for suspension, add 50% sulfuric acid to adjust Ph 3~4, The filtrate was adjusted to pH 6-7 with 40% NaOH, filtered, concentrated to a thick paste, and spray-dried to obtain honeysuckle extract. 3. Take honeysuckle 1kg by ultrafiltration, add (15, 10) times the amount of water, decocting 2 times, 2h each time, filtering, combined filtrate, filtrate concentrated to the original volume of 2/5, the ultrafiltrate was collected and concentrated to a raw dose. The solution was allowed to stand, filtered, concentrated under reduced pressure, and vacuum dried to obtain honeysuckle extract. 4. Reflux extraction with ethanol, weigh 1kg of honeysuckle, put it in a round bottom flask, add (10, 8) times the amount of 70% ethanol, heat reflux extraction 2 times, 2h each time, filter, combine the filtrate, concentrate under reduced pressure, The honeysuckle extract was obtained by vacuum drying. 5. Macroporous adsorption resin treatment to take honeysuckle 1kg, add (15, 10) times the amount of water, 90 ℃ extraction 2 times, 2h each time, filtration, combined filtrate, the filtrate was added to the pretreated macroporous adsorption resin, and eluted with water. The eluate was collected, concentrated under reduced pressure, and vacuum dried to obtain honeysuckle extract. Fig.3 extraction and separation of chlorogenic acid fig.4 extraction and separation of polysaccharide |
| Application | honeysuckle extract is gradually applied in the fields of pharmacy, spices, cosmetics, health food, health drinks, etc, the prospect is very broad. In the pharmaceutical aspect has been developed by the traditional Chinese medicine formula of fried Liu into tablets, injections, pills, granules, ointments and other types: in the perfume industry has developed honeysuckle perfume, honeysuckle shampoo, etc.; In the beverage aspect has developed honeysuckle tea, honeysuckle shower, silver wheat beer spray and so on these products are favored by domestic and foreign consumers. |
| medicinal property | . Return to lung and stomach. The patient was mainly treated with heat-clearing and detoxification, evacuation of wind-heat, cooling blood and stopping dysentery. The patient was diagnosed with extrinsic wind-heat, beginning with blast, Head Pain, thirst, Cough, dry throat, sore throat, sore, erysipelas, heat toxin and blood dysentery.|
| safety toxicology analysis | the toxicity of Flos lonicerae Japonicae is very small. The water extract has no obvious toxicity to rabbits and dogs, blood pressure, urine volume and so on had no effect, mice subcutaneous injection of honeysuckle extract LD50 53 g/kg. Chlorogenic acid has a sensitizing effect, can cause allergic reactions, but oral administration without this reaction, because chlorogenic acid can be converted into small intestinal secretions into non-allergenic substances. The LD50 of chlorogenic acid to young rats was more than 1 g/kg, and the intraperitoneal injection was more than 0.25 g/kg. Hypodermic injection of total saponins of Lonicera fulvotomentosa in mice LD50 was 1.08g/kg, poisoning symptoms of spontaneous activity decreased, respiratory depression died. |
| Use | for organic synthesis and used as pigment, pharmaceutical intermediate |