10199-89-0
NBD chloride
CAS: 10199-89-0
Molecular Formula: C6H2ClN3O3
10199-89-0 - Names and Identifiers
| Name | NBD chloride |
| Synonyms | NBD-Cl NBD chloride 4-Chloro-7-nitrobenzofurazan 4-chloro-7-nitrobenzofurazane 4-Chloro-7-nitrobenzofurazan) 4-chloro-7-nitro-2,1,3-benzoxadiazole 4-chloro-7-nitrobenzo-2,1,3-oxadiazole 2,1,3-Benzoxadiazole, 4-chloro-7-nitro- 4-chloro-7-nitrobenzo[c][1,2,5]oxadiazole 4-CHLORO-7-NITROBENZOFURAZAN, FOR FLUORE SCENCE 7-CHLORO-4-NITROBENZ-2-OXA-1-3-DIAZOLECR YSTALLINE 4-Chloro-7-nitrobenzo-2,1,3-oxadiazole~NBDchloride 4-Chloro-7-nitrobenzo-2,1,3-oxadiazole~NBD chloride 4-Chloro-7-nitro-1,2,3-benzoxadiazole, NBD-chloride 4-Chloro-7-nitrobenz-2-oxa-1,3-diazole (NBD chloride |
| CAS | 10199-89-0 |
| EINECS | 233-496-4 |
| InChI | InChI=1/C6H2ClN3O3/c7-3-1-2-4(10(11)12)6-5(3)8-13-9-6/h1-2H |
10199-89-0 - Physico-chemical Properties
| Molecular Formula | C6H2ClN3O3 |
| Molar Mass | 199.55 |
| Density | 2.0589 (rough estimate) |
| Melting Point | 97-99°C(lit.) |
| Boling Point | 333.1±45.0 °C(Predicted) |
| Flash Point | 155.3°C |
| Water Solubility | soluble |
| Solubility | Soluble in methanol, DMSO, and CHCl3 (50 mg/ml). |
| Vapor Presure | 0.000269mmHg at 25°C |
| Appearance | Yellow crystalline powder |
| Color | Yellow to light brown |
| BRN | 614212 |
| pKa | -5.65±0.50(Predicted) |
| Storage Condition | Inert atmosphere,Room Temperature |
| Refractive Index | 1.5810 (estimate) |
| MDL | MFCD00005808 |
| Use | This product is for scientific research only and shall not be used for other purposes. |
| In vitro study | NBD-Cl (NBD chloride) forms highly fluorescent derivatives for detection of all the protein amino acids. In addition, NBD-Cl provides a simple and sensitive method for determination of N-terminal amino acids. The differences in intensity and color of fluorescence can be used to advantage to identify prolyl peptides. |
10199-89-0 - Risk and Safety
| Risk Codes | R22 - Harmful if swallowed R36/38 - Irritating to eyes and skin. |
| Safety Description | 36/37/39 - Wear suitable protective clothing, gloves and eye/face protection. |
| UN IDs | UN 2811 6.1/PG 3 |
| WGK Germany | 3 |
| RTECS | DF8002400 |
| FLUKA BRAND F CODES | 10-21 |
| TSCA | Yes |
| HS Code | 29349990 |
10199-89-0 - Introduction
4-Chloro-7-nitrobenzofurazan is a dye that forms highly fluorescent compounds upon reaction with amines or thiol compounds. This reagent is used to label peptides, proteins and other biomolecules.
Last Update：2022-10-16 17:11:31
10199-89-0 - Reference Information
| EPA chemical information | Information provided by: ofmpub.epa.gov (external link) |
| Application | 4-chloro-7-nitrobenzo-2-oxa-1, 3-diazole is a highly sensitive color development and fluorescence reagent, Used for chromatographic analysis of amino acids and low molecular weight amines. It is used to prepare fluorescent phospholipid derivatives, hydroxynaphthalene furan and 4-chloro-7-nitrobenzofuran-didecyl phosphatidylethanolamine. |
| Biological activity | NBD-Cl is a non-fluorescent agent that is highly fluorescent after reacting with sulfhydryl or amino groups. |
| use | HPLC fluorescent labeling reagent. For high performance liquid chromatography labeling |
Last Update：2024-04-10 22:29:15
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