3625-57-8
Nile Blue A
CAS: 3625-57-8
Molecular Formula: C20H20N3O5S-
3625-57-8 - Names and Identifiers
| Name | Nile Blue A |
| Synonyms | CI 51180 Nile Blue A CI NO 51180 BASIC BLUE 12 nileblueasulphate BASIC BLUE 12 SULFATE SALT |
| CAS | 3625-57-8 |
| EINECS | 222-832-5 |
3625-57-8 - Physico-chemical Properties
| Molecular Formula | C20H20N3O5S- |
| Molar Mass | 414.46 |
| Melting Point | >300°C (dec.)(lit.) |
| Water Solubility | Soluble in ethanol, and water (50 g/L) at 25°C. |
| Solubility | Soluble in water, ethanol |
| Appearance | Morphology Powder |
| Color | Dark green to black |
| Maximum wavelength(λmax) | 633 nm |
| Storage Condition | room temp |
| MDL | MFCD00064529 |
| Physical and Chemical Properties | Bioactive Nile Blue A (Nile blue sulfate) can be used to distinguish melanin from lipofuscin. It can also be used for fat staining and preparation of current glucose sensors. |
| Use | Use biological staining, distinguish fatty acid and neutral fat, bacterial and actinomycete staining in pathological tissue. It is also used for photometric determination of tantalum. Used in neutralization analysis to prepare mixed indicators. |
| In vitro study | Nile blue A is a basic oxazine dye which is soluble in water and ethyl alcohol. Nile blue A is a satisfactory stain for PHB granules in bacteria and is in fact superior to Sudan black B for this purpose. Poly-p3-hydroxybutyrate granules exhibits a strong orange fluorescence when stained with Nile blue A. Nile blue A appears to stain many more PHB granules than Sudan black B does and is not as easily ished from the cell by decolorization procedures. Nile blue A is used as a stain for polyhydroxyalkanoic acid-accumulating microorganisms or to detect polyhydroxyalkanoic acids in microorganisms. Escherichia coli cells that do not accumulate detectable polyhydroxyalkanoic acids can be stained with Nile blue A and that this staining is sufficient for identifying these cells in fluorescence-activated cell sorting (FACS) experiments. Nile blue A staining does not affect either surface display of peptides or specific labeling of these peptides by a second fluorescence. Staining E. coli for flow cytometry using Nile blue A is an easy-to-handle and low-cost alternative to other fluorescent dyes or the intracellular expression of, for example, green fluorescent protein. Nile blue A is one of the most studied benzophenoxazine dyes, as a potent photosensitizer for photodynamic therapy. The dye when administered intravenously disperses throughout the body by circulating through blood and is taken up by most cells that emphasize its interaction with various biomolecule. |
3625-57-8 - Risk and Safety
| Hazard Symbols | Xi - Irritant |
| Safety Description | S22 - Do not breathe dust. S24/25 - Avoid contact with skin and eyes. |
| WGK Germany | 3 |
| RTECS | DJ1931000 |
3625-57-8 - Upstream Downstream Industry
| Raw Materials | 5-(diethylamino)-2-nitrosophenol 1-Naphthylamine |
3625-57-8 - Reference
| Reference Show more | 1. Li Yang, Xu Qingqing, Han Lu, Wang diqiong, Qi Baokun. Study on emulsifying properties of pea protein isolate with inulin [J]. Journal of Agricultural Machinery, 2020,51(07):382-388. 2. Shu Qiao, Feng Hao. Preparation of cross-linked chitosan microspheres with cinnamaldehyde and controlled release of nobiletin [J]. Journal of Central China Normal University (Natural Science Edition), 2018, v.52;No.182(06):46-52. 3. [IF = 9.147] Xianling Wei et al."Fabrication and characterization of simulations stabilized by tannic acid-wheat starch complexes." Food Hydrocolloid. 2020 Oct;107:105728 4. [IF = 9.147] Shulin Zhang et al."Mixed plant-based emulsifiers inhibit the oxidation of proteins and lipids in walnut oil-in-water fluctuations." Food Hydrocolloid. 2020 Dec;109:106136 5. [IF=9.147] Fang Zhang et al."Effect of pH, ionic strength, chitosan deacetylation on the stability and rheological properties of O/W emulsions formulated with chitosan/casein complexes."Food Hydrocolloid. 2021 Feb;111:106211 6. [IF=9.147] Yangyue Ding et al."Effects of endogenous proteins and lipids on structural, thermal, rheological, and pasting properties and digestibility of adlay seed (Coix lacryma-jobi L.) starch."Food Hydrocolloid. 2021 Feb;111:106254 7. [IF=9.147] Anqi Li et al."Gelation mechanism and physical properties of glucono-δ-lactone induced alginate sodium/casein composite gels."Food Hydrocolloid. 2021 Sep;118:106775 8. [IF=9.147] Peiyuan Li et al."Preparation and structural characteristics of composite alginate/casein emulsion gels: A microscopy and rheology study."Food Hydrocolloid. 2021 Sep;118:106792 9. [IF=7.514] Xiaofei Li et al."Acid-induced mixed methylcellulose and casein gels: Structures, physical properties and formation mechanism."Food Chem. 2022 Jan;366:130561 10. [IF=6.953] Xi Yang et al."Structuring oil-in-water emulsion by forming egg yolk/alginate complexes: Their potential application in fabricating low-fat mayonnaise-like emulsion gels and redispersible solid emulsions."Int J Biol Macromol. 2020 Mar;147:595 11. [IF=6.953] Huaping Yu et al."Cellulose nanocrystals based clove oil Pickering emulsion for enhanced antibacterial activity."Int J Biol Macromol. 2021 Feb;170:24 12. [IF=4.952] Ruihong Wang et al."Changes in egg yolk gelation behaviour and mechanisms during freezing."Lwt Food Sci Technol. 2021 Nov;151:112223 13. [IF=5.916] Ning Liu et al."Combined effects of limited enzymatic hydrolysis and high hydrostatic pressure on the structural and emulsifying properties of rice proteins."Innov Food Sci Emerg. 2022 May;77:102975 14. [IF=4.952] Wei Xu et al."Stabilization and microstructural network of pickering emulsion using different xanthan gum/lysozyme nanoparticle concentrations."Lwt Food Sci Technol. 2022 Apr;160:113298 15. [IF=6.576] Wei Xu et al."High Internal-Phase Pickering Emulsions Stabilized by Xanthan Gum/Lysozyme Nanoparticles: Rheological and Microstructural Perspective."Front Nutr. 2021; 8: 744234 16. [IF=1.713] Kai Yuan et al."Effect of bacterial cellulose nanofibers incorporation on acid-induced casein gels: microstructures and rheological properties."Int J Food Eng. 2021 Dec;: 17. [IF=7.514] Chun-Min Ma et al."Impact of covalent grafting of two flavonols (kaemperol and quercetin) to caseinate on in vitro digestibility and emulsifying properties of the caseinate-flavonol grafts."FOOD CHEMISTRY. 2022 Oct;390:133210 |
3625-57-8 - Nature
| pH range of acid-base indicator discoloration | 10.1(BLUE)-11.1(RED) |
| maximum wavelength (& lambda;max) | 633 nm |
| biological applications | Detecting microorganisms; treating virus infectious diseases; photodynamic therapy |
Last Update：2024-04-10 22:29:15
3625-57-8 - Uses and synthesis methods
in vitro studies
Nile blue A is a basic oxazine dye which is soluble in water and ethyl alcohol. Nile blue A is a satisfactory stain for PHB granules in bacteria and is in fact superior to Sudan black B for this purpose. Poly-p3-hydroxybutyrate granules exhibits a strong orange fluorescence when stained with Nile blue A. Nile blue A appears to stain many more PHB granules than Sudan black B does and is not as easily ished from the cell by decolorization procedures. Nile blue A is used as a stain for polyhydroxyalkanoic acid-accumulating microorganisms or to detect polyhydroxyalkanoic acids in microorganisms. Escherichia coli cells that do not accumulate detectable polyhydroxyalkanoic acids can be stained with Nile blue A and that this staining is sufficient for identifying these cells in fluorescence-activated cell sorting (FACS) experiments. Nile blue A staining does not affect either surface display of peptides or specific labeling of these peptides by a second fluorescence. Staining E. coli for flow cytometry using Nile blue A is an easy-to-handle and low-cost alternative to other fluorescent dyes or the intracellular expression of, for example, green fluorescent protein. Nile blue A is one of the most studied benzophenoxazine dyes, as a potent photosensitizer for photodynamic therapy. The dye when administered intravenously disperses throughout the body by circulating through blood and is taken up by most cells that emphasize its interaction with various biomolecule.
Last Update：2024-04-10 22:29:15
3625-57-8 - Introduction
Nile Blue A, also known as Nielsen reagent, is an organic dye. The following is a detailed introduction:
Nature:
- Nile Blue A is A dark Blue crystalline powder with good solubility.
-It can be dissolved in water, but insoluble in organic solvents.
-It changes color under acidic conditions and turns red.
Use:
- Nile Blue A is widely used as A dye, especially in biological science and chemical experiments.
-it can be used to detect the presence of DNA or RNA, analysis of nickel ions and reducing sugar and other chemical experiments.
-In biology, Nile Blue A can be used to prepare buffers for gel electrophoresis.
Preparation Method:
- Nile Blue A has many preparation methods, and the commonly used method is to obtain it by reacting chloracetyl benzylamine with nitrobenzophenone.
Safety Information:
- Nile Blue A is generally considered to be relatively safe.
-However, it may be irritating to the eyes, skin and respiratory system. Therefore, care should be taken to avoid contact with eyes, skin and inhalation of its dust.
-If contact or inhalation occurs, rinse immediately with plenty of water and seek medical help.
-During use, please follow the correct laboratory procedures and wear appropriate safety equipment, such as gloves, goggles and protective clothing.
Nature:
- Nile Blue A is A dark Blue crystalline powder with good solubility.
-It can be dissolved in water, but insoluble in organic solvents.
-It changes color under acidic conditions and turns red.
Use:
- Nile Blue A is widely used as A dye, especially in biological science and chemical experiments.
-it can be used to detect the presence of DNA or RNA, analysis of nickel ions and reducing sugar and other chemical experiments.
-In biology, Nile Blue A can be used to prepare buffers for gel electrophoresis.
Preparation Method:
- Nile Blue A has many preparation methods, and the commonly used method is to obtain it by reacting chloracetyl benzylamine with nitrobenzophenone.
Safety Information:
- Nile Blue A is generally considered to be relatively safe.
-However, it may be irritating to the eyes, skin and respiratory system. Therefore, care should be taken to avoid contact with eyes, skin and inhalation of its dust.
-If contact or inhalation occurs, rinse immediately with plenty of water and seek medical help.
-During use, please follow the correct laboratory procedures and wear appropriate safety equipment, such as gloves, goggles and protective clothing.
Last Update：2024-04-09 20:48:19
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Featured ProductsSpot supply
Product Name: Nile Blue indicator solution Visit Supplier Webpage Request for quotationCAS: 3625-57-8
Tel: +86-18821248368
Email: Int06@meryer.com
Mobile: +86-18821248368
QQ: 495145328
WhatsApp: +86-18821248368
Spot supply
Product Name: NileBlueAsulfate Request for quotationCAS: 3625-57-8
Tel: 400-968-2212
Email: 3623107365@qq.com
Mobile: 18916960931
QQ: 3623107365
Wechat: 18916960931
Spot supply
Product Name: Nile Blue A Visit Supplier Webpage Request for quotationCAS: 3625-57-8
Tel: +86-400-900-4166
Email: product@acmec-e.com
Mobile: +86-18621343501
QQ: 2881950922
Wechat: 18621343501
WhatsApp: +86-18621343501
Spot supply
Product Name: Nile Blue A sulfate Visit Supplier Webpage Request for quotationCAS: 3625-57-8
Tel: 609-228-6898
Email: sales@medchemexpress.com
tech@medchemexpress.com
Mobile: 609-228-6898
Spot supply
Product Name: Nile Blue A Visit Supplier Webpage Request for quotationCAS: 3625-57-8
Tel: +86 0571-86722205
Email: sales@chinaskyrun.com
Mobile: +8618958170122
QQ: 2531159185
Wechat: chinaskyrun
Spot supply
Product Name: Nile Blue A Visit Supplier Webpage Request for quotationCAS: 3625-57-8
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
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Raw Materials for 3625-57-8