BENZONASE(R)
MUNG BEAN NUCLEASE
CAS: 9025-65-4
Molecular Formula:
BENZONASE(R) - Names and Identifiers
| Name | MUNG BEAN NUCLEASE |
| Synonyms | R P1 S1 EC 3.1.30.1 3.1.30.2 NUCLEASE BENZONASE ENDONUCLEASE BENZONASE(R) MUNG BEAN NUCLEASE |
| CAS | 9025-65-4 |
BENZONASE(R) - Physico-chemical Properties
| Storage Condition | -20℃ |
BENZONASE(R) - Risk and Safety
| Safety Description | S23 - Do not breathe vapour. S24/25 - Avoid contact with skin and eyes. |
BENZONASE(R) - Mung bean nuclease cuts off Plasmodium genomic DNA before and after the gene
From HowNet
Author:
Summary:
Mung Bean nuclease (Mung Bean Nuclease) can cut off the genomic DNA of malaria protozoa Plasmodium before and after the gene but not in the coding region of the gene. This cutting has almost the accuracy of restriction endonuclease and requires formamide control. Southern blotting showed that the gene coding regions of actin, CS protein (circum-sporozoite protein), histidine-rich egg, ribosomal RNA-binding protein and tubulin of Plasmodium were all cut from genomic DNA one by one to form a single narrow band on agarose gel. By
keywords:
mung bean nuclease Plasmodium genome protein gene restriction endonuclease actin binding protein agarose gel gene coding region blot method
Year:
1985
Last Update：2024-01-02 23:10:35
BENZONASE(R) - Extraction and Activity Determination of Nuclease from Mung Bean
from Baidu library
Author:
Hu Min ,< a href = "https://xueshu.baidu.com/usercenter/data/author?cmd=authoruri&wd=authoruri:(1602588 bf696e8bd) author:(Zhao Xiaoju)" target = "_blank"> Zhao Xiaoju ,< a href = "https://xueshu.baidu.com/usercenter/data/author?cmd=authoruri&wd=authoruri:(2 c8dfa2cf7abcc4f) author:(Zhang Yiting)" target = "_blank">/a >,< a href = "https://xueshu.baidu.com/usercenter/data/author?cmd=authoruri&wd=authoruri:(7194727 e747df228) author:(fan Xue)" target = "_blank"> fan Xue ,< a href = "https://xueshu.baidu.com/usercenter/data/author?cmd=authoruri&wd=authoruri:(e0244d549ff4c597) author:(Li Xu)" target = "_blank"> Li Xu
Summary:
objective] to optimize the extraction conditions of mung bean nuclease and determine its activity. [method] single factor and orthogonal test were used to investigate the effects of extraction time, solid-liquid ratio, pH and bud length on the extraction of nuclease from mung bean sprouts. the optimal level of each influencing factor was optimized and the activity was determined. [results] the extraction time was 20 h and the solid-liquid ratio was 1:12: mL),pH 3.0, bud length is 4 times that of mung bean, mung bean nuclease activity is 478.86U/ mL. The main order of factors affecting the extraction of mung bean nuclease is extraction time, pH, solid-liquid ratio and bud length. After ammonium sulfate with saturation of 80% was salted out and precipitated, the enzyme activity reached 1 569.6U/ mL, which was 2.28 times higher than that before purification. [conclusion] the study can provide reference for the development and utilization of nuclease.
keywords:
mung bean nuclease extract vitality
DOI:
10.3969/j.issn.0517-6611.2018.02.043
Year:
2018
Last Update：2023-08-16 22:23:51
BENZONASE(R) - Introduction
Unit definition: The amount of enzyme that reduces the △ A260 value by 1.0 (equivalent to complete digestion of 37 μg of DNA) within 30 minutes is defined as an active unit; The optimum pH is 8.0-9.2, the optimum temperature is 37, and the optimum Mg2 concentration is 1-2mM; Inactivation conditions: cannot be completely inactivated.
Last Update：2022-10-16 17:30:39
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