| Use | The construction of the electrochemical aptamer sensor based on the intramolecular configuration transformation is mainly based on the specific recognition of the target by the nucleic acid aptamer immobilized on the electrode surface, and the aptamer configuration changes, modifying the electrical activity at the end of the aptamer, the molecule is close to or distant from the electrode surface. There are two main types of signal amplification strategies based on nuclease-assisted. One is the sequence-dependent restriction endonuclease or cleavage endonuclease-assisted signal amplification strategy, the other is a sequence-independent exonucleation-assisted signal amplification strategy. In terms of the versatility of the nuclease-assisted signal amplification strategy, the exonuclease without sequence dependence is more simple in probe design and target analysis, thus showing a wider application prospect. |