中文名 | REACTIVE BLUE 2 |
英文名 | REACTIVE BLUE 2 |
别名 | 蓝色染料 兰色染料 活性蓝2 汽巴蓝F3GA C.I.活性蓝2 化合物 T19049 汽巴蓝 3GA 琼脂糖 |
英文别名 | REACTIVE BLUE 2 CIBRACONBLUEF3GA Kayacion Blue A-B C.I.REACTIVEBLUE2 Procion Blue HB, pure Reactive blue 2 (C.I. 61211) REACTIVE BLUE 2 C.I. NO. 61211 2-Anthracenesulfonic acid, 1-amino-4-4-4-chloro-6-3(or 4)-sulfophenylamino-1,3,5-triazin-2-ylamino-3-sulfophenylamino-9,10-dihydro-9,10-dioxo- 1-Amino-4-[[4-[[4-chloro-6-[[4-(sodiooxysulfonyl)phenyl]amino]-1,3,5-triazin-2-yl]amino]-3-(sodiooxysulfonyl)phenyl]amino]-9,10-dihydro-9,10-dioxo-2-anthracenesulfonic acid sodium salt |
CAS | 12236-82-7 |
EINECS | 235-465-0 |
化学式 | C29H20ClN7O11S3 |
分子量 | 774.16 |
溶解度 | H2O: 可溶 |
存储条件 | 2-8°C |
稳定性 | 稳定。与强氧化剂不相容。 |
外观 | 盐水悬浮液 |
颜色 | dark blue |
体外研究 | Reactive Blue 2 is used as an ATP receptor antagonist and induces Ca 2+ oscillations in HeLa cells. Reactive Blue 2 enhances a Ca 2+ response to histamine that is linked to the PLC cascade. Reactive Blue 2 may activate the PLC cascade in an extracellular Ca 2+ -dependent manner and induce Ca 2+ oscillations. The application of Reactive Blue 2 increases K + secretion in a dose-dependent manner, and this increase is characterized as a peak followed by a partial relaxation to a steady-state. Reactive Blue 2 has antagonistic activities at P2Y4, and the antagonist potency at P2Y4 paralleled the potency of K+ secretion.The anthraquinone dye reactive blue 2 is found to be a potent inhibitor of a protein kinase isolated and purified from thylakoids. The mode of inhibition is noncompetitive, with a K i of 8 μM for the membrane-bound kinase, and 6 microM for the purified kinase. The inhibitor does not modify the substrate preference of the endogenous kinase and could be removed from the membrane by washing. Reactive blue 2 selectively inhibits responses mediated via the P2ypurinoceptor, at least within a limited concentration range. In preparations where the tone has been raised with noradrenaline, ATP and 2-methylthio ATP, but not α,β-methylene ATP, produce relaxations of the vessel. These relaxations are inhibited in the presence of reactive blue 2. Reactive blue 2, at concentrations of 0.3-10 μM blocks the ATP-induced oscillation in a concentration-dependent manner. |
危险品标志 | Xi - 刺激性物品![]() |
风险术语 | 36/37/38 - 刺激眼睛、呼吸系统和皮肤。 |
安全术语 | S26 - 不慎与眼睛接触后,请立即用大量清水冲洗并征求医生意见。 S36 - 穿戴适当的防护服。 |
WGK Germany | 3 |
FLUKA BRAND F CODES | 10-21 |
上游原料 | 2,5-二氨基苯磺酸 3-氨基苯磺酸 溴氨酸 三聚氯氰 |
参考资料 展开查看 | 1. [IF=0] Dong-Hao Zhang et al."Effects of different spacer arms on Cibacron Blue modification and protein affinity adsorption on magnetic microspheres."J Mol Catal B Enzym. 2016 Nov;133:136 2. [IF=5.328] Li Xin Ping et al."The signaling role of extracellular ATP in co-culture of Shiraia sp. S9 and Pseudomonas fulva SB1 for enhancing hypocrellin A production."Microb Cell Fact. 2021 Dec;20(1):1-15 3. [IF=3.174] Yu-Pei Xia et al."An efficient protein immobilization strategy: protein encapsulated in nano molecular cages."J Chem Technol Biot. 2019 Jul;94(7):2248-2255 |
1mg | 5mg | 10mg | |
---|---|---|---|
1 mM | 1.292 ml | 6.459 ml | 12.917 ml |
5 mM | 0.258 ml | 1.292 ml | 2.583 ml |
10 mM | 0.129 ml | 0.646 ml | 1.292 ml |
5 mM | 0.026 ml | 0.129 ml | 0.258 ml |
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