中文名 | Dp44mT |
英文名 | Dp44mT |
别名 | 2-(二-2-吡啶基亚甲基)-N,N-二甲基肼基硫代甲酰胺 |
英文别名 | Dp44mT de44mt CS-2521 Iron Chelator Iron Chelator, Dp44mT Di-2-pyridylketone-4,4,-dimethyl-3-thiosemicarbazone 2-(Di-2-pyridinylmethylene)-N,N-dimethyl-hydrazinecarbothioamide Hydrazinecarbothioamide, 2-(di-2-pyridinylmethylene)-N,N-dimethyl- |
CAS | 152095-12-0 |
EINECS | 694-427-5 |
化学式 | C14H15N5S |
分子量 | 285.37 |
溶解度 | DMSO: ≥ 5 mg/mL |
存储条件 | 2-8°C |
稳定性 | 从提供的购买之日起稳定1年。在DMSO中的溶液可以在-20 °C下储存1个月。 |
敏感性 | Light Sensitive |
外观 | 粉末 |
颜色 | yellow to orange |
体外研究 | Dp44mT is cytotoxic to breast cancer cells, at least in part, due to selective inhibition of top2α. Dp44mT alone induced selective cell killing in the breast cancer cell line MDA-MB-231 when compared with healthy mammary epithelial cells (MCF-12A). It induces G1 cell cycle arrest and reduces cancer cell clonogenic growth at nanomolar concentrations. Dp44mT, but not the iron chelator desferal, induces DNA double-strand breaks quantified as S139 phosphorylated histone foci (γ-H2AX) and Comet tails induced in MDA-MB-231 cells. Doxorubicin-induced cytotoxicity and DNA damage are both enhanced significantly in the presence of low concentrations of Dp44mT. The chelator caused selective poisoning of DNA topoisomerase IIα (top2α) as measured by an in vitro DNA cleavage assay and cellular topoisomerase-DNA complex formation. Dp44mT targets lysosome integrity through copper binding. Copper binding is essential for the potent antitumor activity of Dp44mT, as coincubation with nontoxic copper chelators markedly attenuated its cytotoxicity. |
危险品标志 | Xn - 有害物品 |
风险术语 | 22 - 吞食有害。 |
危险品运输编号 | UN 2811 6.1 / PGIII |
WGK Germany | 3 |
Hazard Class | 6.1 |
1mg | 5mg | 10mg | |
---|---|---|---|
1 mM | 3.504 ml | 17.521 ml | 35.043 ml |
5 mM | 0.701 ml | 3.504 ml | 7.009 ml |
10 mM | 0.35 ml | 1.752 ml | 3.504 ml |
5 mM | 0.07 ml | 0.35 ml | 0.701 ml |
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