3-(4,5-二甲基-2-噻唑)-2,5-二苯基四氮唑溴盐(MTT)
噻唑蓝溴化四唑(MTT)
CAS: 298-93-1;2348-71-2
化学式: C18H16BrN5S
| 中文名 | 噻唑蓝溴化四唑 |
| 英文名 | MTT |
| 别名 | 噻唑蓝 噻唑兰 噻唑蓝溴化四唑 溴化噻唑蓝四氮唑 噻唑四唑蓝溴化盐 3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐 3-(4,5-二甲基-2-噻唑)-2,5-二苯基四氮唑溴盐 3-(4,5-二甲基-2-噻唑)-2,5-二苯基溴化四氮唑 3-(4,5-二甲基-2-噻唑基)-2,5-二苯基四氮唑溴化物 溴化-3-(4,5-二甲基-2-噻唑基)-2,5-二苯基四氮唑 3-(4,5-二甲基-2-噻唑)-2,5-二苯基四氮唑溴盐(MTT) 3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2H-四氮唑溴化物 3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2H-四氮唑鎓溴化物 3-(4,5-二甲基-2-噻唑)-2,5-二苯基溴化四氮唑噻唑蓝,噻唑兰,溴化噻唑蓝四氮唑 |
| 英文别名 | MTT MTT (1.11714) Thiazolyl Blue METHYLTHIAZOLETETRAZOLIUM Thiazolyl Blue tetrazolium bromide 3-(4,5-Dimethyl-2-Thiazolyl)-2,5-Diphenyl 3-(4,5-Dimethyl-2-thizolyl)-2,5-diphenyltertazolium bromide 3,[4,5-Dimethylthiazol-2-YL]-2,5-DIPHENYLTETRAZOLIUM BROMIDE 2,5-diphenyl-3-(4,5-dimethylthiazol-2-yl)tetrazolium Bromide 3-(4,5-Dimethylthiazolyl-2)-2,5-DIPHENYL TETRAZOLIUM BROMIDE 3-(4,5-Dimethyl-2-THIAZOLYL)-2,5-DIPHENYLTETRAZOLIUM BROMIDE 3-(4,5-Dimethylthiazol-2-YL)-2,5-DIPHYL-2 H-TETRAZOLIUM BROMIDE 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2h-tetrazoliumbromide 2,5-diphenyl-3-(4,5-dimethylthiazol-2-yl)-2h-tetrazolium Bromide 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide 3-(4,5-DIMETHYLITHIAZOLYL-2)-2,5-DIPHENYL MONOTETRAZOLIUM BROMIDE 3-(4,5-Dimethyl-2-THIAZOL-2-YL)-2,5-DIPHENYL-2H-TETRAZOLIUM BROMIDE 2-(4,5-Dimethyl-2-thiazolyl)-3,5-diphenyl-2H-tetrazol-3-ium Bromide 3-(4,5-dimethyl-1,3-thiazol-2-yl)-2,5-diphenyl-2H-tetrazol-3-ium bromide 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-2,3-dihydro-1H-tetrazol-3-ium bromide 2-(4,5-Dimethylthiazol-2-yl)-3,5-diphenyl-2,3-dihydro-1H-tetrazol-3-ium bromide 3-(4,5-dimethyl-1,3-thiazol-2-yl)-2,5-diphenyl-2,3-dihydro-1H-tetrazol-1-ium bromide |
| CAS | 298-93-1 2348-71-2 |
| EINECS | 206-069-5 |
| 化学式 | C18H16BrN5S |
| 分子量 | 414.32 |
| InChI | InChI=1/C18H17N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3,(H,20,21);1H |
| InChIKey | AZKSAVLVSZKNRD-UHFFFAOYSA-M |
| 密度 | 1.4886 (estimate) |
| 熔点 | 195 °C (dec.) (lit.) |
| 水溶性 | Slightly soluble.Clear yellow solution at 5mg in 1ml of water on vortexing |
| 溶解度 | 甲醇: 5mg/ml，澄清 |
| 存储条件 | 2-8°C |
| 稳定性 | 吸湿性 |
| 敏感性 | Light Sensitive |
| 外观 | 粉末 |
| 颜色 | Yellow to orange |
| 最大波长(λmax) | ['378 nm, 242 nm'] |
| BRN | 3825277 |
| MDL号 | MFCD00011964 |
| 体外研究 | Thiazolyl Blue (MTT) is used in conjunction with rhodamine B to measure mitochondrial membrane potential. Thiazolyl Blue-formazan is generated in mitochondria acts as a fluorescence quencher for rhodamine that distributes across membranes of viable cells according to membrane potential. In the absence of mPMS, cellular reduction of Thiazolyl Blue is strong. Thiazolyl Blue is membrane-impermeable when incorporated into large unilamellar liposomes, and therefore it is taken up by cells via endocytosis. |
| 危险品标志 | Xi - 刺激性物品 T - 有毒物品  Xn - 有害物品  |
| 风险术语 | R46 - 可能引起遗传性基因损害。 R68 - 可能有不可逆后果的危险。 R36/37/38 - 刺激眼睛、呼吸系统和皮肤。 |
| 安全术语 | S22 - 切勿吸入粉尘。 S24/25 - 避免与皮肤和眼睛接触。 S53 - 避免接触，使用前须获得特别指示说明。 S45 - 若发生事故或感不适，立即就医(可能的话，出示其标签)。 S36/37 - 穿戴适当的防护服和手套。 S26 - 不慎与眼睛接触后，请立即用大量清水冲洗并征求医生意见。 |
| WGK Germany | 3 |
| RTECS | XF8060000 |
| FLUKA BRAND F CODES | 3-8-10 |
| TSCA | Yes |
| 海关编号 | 29341000 |
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3-(4,5-二甲基-2-噻唑)-2,5-二苯基四氮唑溴盐(MTT) - 介绍
常用于基于MTT检测方法的细胞活力、细胞增殖或细胞毒性检测。因为MTT具有正电荷，它可以穿过细胞膜并被线粒体还原形成紫色甲臜染料。溶解MTT甲臜染料需要使用有机溶剂。脱氢酶的检测，生化研究中酶活力的测定，测定NADH2、NADPH2和心肌黄酶(Diaphorase)。
最后更新：2022-10-16 17:24:22
3-(4,5-二甲基-2-噻唑)-2,5-二苯基四氮唑溴盐(MTT) - 简介
噻唑蓝溴化四唑是一种有机合成化合物。它是一种深蓝色的晶体固体，不溶于水，溶于有机溶剂如甲醇、氯仿等。
噻唑蓝溴化四唑主要用于荧光探针、DNA染料等方面。它可以通过与DNA中G-C碱基对特异性结合而发光，并用于DNA凝胶电泳、PCR扩增等实验室技术中。
制备噻唑蓝溴化四唑一般采用化学合成方法。具体做法是将2-甲基-4-硝基苯胺与硫氰酸钠反应得到2-甲基-4-硫代噻唑，然后与偶氮二乙酸酯、高氯酸铵反应生成中间体，最后通过甲苯磺酰氯和溴反应制得噻唑蓝溴化四唑。
噻唑蓝溴化四唑在使用时需注意安全。它具有一定的毒性和刺激性，需要避免直接接触皮肤或吸入。在操作过程中应佩戴防护手套、眼镜和口罩等个人防护用具，并确保通风良好。另外，噻唑蓝溴化四唑应储存在干燥、阴凉处，远离火源和氧化剂。
噻唑蓝溴化四唑主要用于荧光探针、DNA染料等方面。它可以通过与DNA中G-C碱基对特异性结合而发光，并用于DNA凝胶电泳、PCR扩增等实验室技术中。
制备噻唑蓝溴化四唑一般采用化学合成方法。具体做法是将2-甲基-4-硝基苯胺与硫氰酸钠反应得到2-甲基-4-硫代噻唑，然后与偶氮二乙酸酯、高氯酸铵反应生成中间体，最后通过甲苯磺酰氯和溴反应制得噻唑蓝溴化四唑。
噻唑蓝溴化四唑在使用时需注意安全。它具有一定的毒性和刺激性，需要避免直接接触皮肤或吸入。在操作过程中应佩戴防护手套、眼镜和口罩等个人防护用具，并确保通风良好。另外，噻唑蓝溴化四唑应储存在干燥、阴凉处，远离火源和氧化剂。
最后更新：2024-04-10 22:29:15
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