中文名 | CPTH2 |
英文名 | CPTH2 |
别名 | 4-(4-氯苯基)-2-(2-环戊烯肼基)噻唑 |
英文别名 | CPTH2 Histone Acetyltransferase Inhibitor IV, CPTH2 Cyclopentylidene-[4-(4-chlorophenyl)thiazol-2-yl)hydrazone Cyclopentanone, 2-[4-(4-chlorophenyl)-2-thiazolyl]hydrazone 4-(4-CHLOROPHENYL)-N-(CYCLOPENTYLIDENEAMINO)-1,3-THIAZOL-2-AMINE |
CAS | 357649-93-5 |
化学式 | C14H14ClN3S |
分子量 | 291.8 |
密度 | 1.38±0.1 g/cm3(Predicted) |
熔点 | 190-191 °C |
沸点 | 460.3±37.0 °C(Predicted) |
溶解度 | 溶于DMSO |
酸度系数 | 14.57±0.20(Predicted) |
存储条件 | 2-8°C |
稳定性 | 从提供的购买之日起稳定1年。DMSO中的溶液可以在-20 ° 下储存长达1个月。 |
敏感性 | Light Sensitive |
外观 | 粉末 |
颜色 | white to beige |
体外研究 | CPTH2 (100 μM; 12, 24, 48 hours) causes a decrease in cell proliferation after as early as 12 h with a further significant reduction after 48 h stimulation. CPTH2 (100 μM; 12 or 48 hours) causes a comparable drop of the activity in both cell lines. CPTH2 (100 μM; 48 hours) produces a drastic increase in apoptotic/dead cell population after 48 h. CPTH2 (100 μM; 12, 24, 48 hours) shows a reduced acetylation of both global AcH3 histone and H3AcK18. CPTH2 (100 μM; 24, 48 hours) is capable to counteract invasion and migration of ccRCC-786-O cells in culture. CPTH2 (0.2, 0.5, 1 mM) inhibits the growth of a GCN5 deleted strain and a single catalytic mutant E173H. CPTH2 (0.6, 0.8 mM; for 24 hours) inhibits histone H3 acetylation in yeast cell cultures. CPTH2 inhibits the Gcn5p dependent functional network. Cell Proliferation Assay Cell Line: Papillary thyroid (K1) and clear cell Renal Cell Carcinoma (ccRCC-786-O) cell lines Concentration: 100 μM Incubation Time: 12, 24, 48 hours Result: Caused a decrease in cell proliferation after as early as 12 h with a further significant reduction after 48 h stimulation. Cell Viability Assay Cell Line: K1 and ccRCC-786-O cell lines Concentration: 100 μM Incubation Time: 24 hours (K1 cell) and 48 hours (ccRCC-786-O cell) Result: Caused a comparable drop of the activity in both cell lines. Apoptosis Analysis Cell Line: ccRCC-786-O cells Concentration: 100 μM Incubation Time: 48 hours Result: Produced a drastic increase in apoptotic/dead cell population after 48 h. Western Blot Analysis Cell Line: ccRCC-786-O cells Concentration: 100 μM Incubation Time: 12, 24, 48 hours Result: Showed a reduced acetylation of both global AcH3 histone and H3AcK18. |
WGK Germany | nwg |
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