中文名 | H-ASP-ALA-GLU-PHE-ARG-HIS-ASP-SER-GLY-TYR-GLU-VAL-HIS-HIS-GLN-LYS-LEU-VAL-PHE-PHE-ALA-GLU-ASP-VAL-GLY-SER-ASN-LYS-OH |
英文名 | H-ASP-ALA-GLU-PHE-ARG-HIS-ASP-SER-GLY-TYR-GLU-VAL-HIS-HIS-GLN-LYS-LEU-VAL-PHE-PHE-ALA-GLU-ASP-VAL-GLY-SER-ASN-LYS-OH |
别名 | Β淀粉样蛋白(1-28) AMYLOID Β肽(1-28) Β淀粉样肽(1-28)单克隆抗体 Β-淀粉样蛋白 AMYLOID Β-PROTEIN (1-28) |
英文别名 | SP28 Β-AMYLOID (1-28) BETA-AMYLOID (1-28) DAEFRHDSGYEVHHQKLVFFAEDVGSNK amyloid B-protein fragment 1-28 BETA-AMYLOID PEPTIDE [1-28], HUMAN ALZHEIMER'S DISEASE Β-PROTEIN (SP28) BETA-AMYLOID (1-28), N-TERMINAL UNLABELED ASP-ALA-GLU-PHE-ARG-HIS-ASP-SER-GLY-TYR-GLU-VAL-HIS-HIS-GLN-LYS-LEU-VAL-PHE-PHE-ALA-GLU-ASP-VAL-GLY-SER-ASN-LYS H-ASP-ALA-GLU-PHE-ARG-HIS-ASP-SER-GLY-TYR-GLU-VAL-HIS-HIS-GLN-LYS-LEU-VAL-PHE-PHE-ALA-GLU-ASP-VAL-GLY-SER-ASN-LYS-OH |
CAS | 109770-29-8 |
化学式 | C145 H209 N41 O46 |
分子量 | 3262.46 |
密度 | 1.52±0.1 g/cm3(Predicted) |
存储条件 | −20°C |
外观 | 白色冻干粉。 |
体外研究 | β-Amyloid Aggregation Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1. Solid Aβ peptide was dissolved in cold hexafluoro-2-propanol (HFIP). The peptide was incubated at room temperature for at least 1h to establish monomerization and randomization of structure. 2. The HFIP was removed by evaporation, and the resulting peptide was stored as a film at -20 or -80 ℃. 3. The resulting film was dissolved in anhydrous DMSO at 5 mM and then diluted into the appropriate concentration and buffer (serum- and phenol red-free culture medium) with vortexing. 4. Next, the solution was age 48h at 4-8 ℃. The sample was then centrifuged at 14000g for 10 min at 4-8 ℃; the soluble oligomers were in the supernatant. The supernatant was diluted 10-200-fold for experiments. |
体内研究 | β-Amyloid (1-28) fragment is considered as valid models to examine the contribution of the key histidine residues (His , His in mouse and His , His , His in human fragments) to the Ab–Cu 2+ interaction. Copper binds to β-Amyloid (1-28) at pH 7.4 with an affinity of K a 10 7 M -1 . |
WGK Germany | 3 |
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