1: Yu K, Toral-Barza L, Shi C, Zhang WG, Lucas J, Shor B, Kim J, Verheijen J, Curran K, Malwitz DJ, Cole DC, Ellingboe J, Ayral-Kaloustian S, Mansour TS, Gibbons JJ, Abraham RT, Nowak P, Zask A. Biochemical, cellular, and in vivo activity of novel ATP-competitive and selective inhibitors of the mammalian target of rapamycin. Cancer Res. 2009 Aug 1;69(15):6232-40. doi: 10.1158/0008-5472.CAN-09-0299. Epub 2009 Jul 7. PubMed PMID: 19584280.
WYE-687 - 配置溶液浓度参考
1mg
5mg
10mg
1 mM
1.892 ml
9.459 ml
18.918 ml
5 mM
0.378 ml
1.892 ml
3.784 ml
10 mM
0.189 ml
0.946 ml
1.892 ml
5 mM
0.038 ml
0.189 ml
0.378 ml
最后更新:2024-01-02 23:10:35
WYE-687 - 细胞实验
For cell cycle analysis, cells are seeded in 96-well culture plates at 10,000 cells per well for 24 hours, treated with various inhibitors for 24 hours or 48 hours. Following treatment, cells are harvested, washed with PBS and fixed overnight at -20 °C in 70% ethanol. Cells are washed, stained with propidium iodide and analyzed for cell cycle profile (acquired 5000 cells/well) on Guava PCA-96 instrument according to the Guava Cell Cycle Protocol(Only for Reference)