脂多糖
脂多糖(Lipopolysaccharides)
CAS:
化学式:
| 中文名 | 脂多糖 |
| 英文名 | Lipopolysaccharides |
| 别名 | 脂多糖 |
| 英文别名 | Lipopolysaccharides LIPOPOLYSACCHARIDE FROM ESCHERICHIA COLI |
| 溶解度 | 5 mg/mlWater；:可溶于水(10mg/ml)或细胞培养基(1mg/ml)，产生浑浊淡黄色溶液，溶于盐溶液经旋涡振荡并加热到70-80℃可得到一种浓度更高的LPS溶液（20mg/ml）。1. |
| 存储条件 | 2-8°C |
| 外观 | 冻干粉 |
| 危险品标志 | Xn - 有害物品 |
| WGK Germany | 3 |
| 参考资料 展开查看 | 1. 黄敏玉 黄华武 黄群 et al. TGF-β1在人前列腺上皮细胞炎症过程中的表达机制及意义[J]. 暨南大学学报（自然科学与医学版） 2020 041(001):39-47. 2. 刘世发 王荣梅 焦洪超 赵景鹏 王晓鹃 林海.免疫应激对蛋鸡钙磷代谢的影响[J].动物营养学报 2015 27(07):2201-2208. 3. 范慧婕,谭章斌,梁红峰,刘彬,赵晓山,周迎春.四逆散对脂多糖诱导的RAW264.7细胞极化的影响[J].中国实验方剂学杂志,2019,25(13):9-14. 4. 范慧婕,谭章斌,赵晓山,梁红峰,刘彬,周迎春.四逆散通过MAPKs/NF-κB途径保护脂多糖致Raw264.7的细胞炎症[J].暨南大学学报(自然科学与医学版),2019,40(01):10-18. 5. 李中英,张丙宏,唐荣,凌伟.新生鼠坏死性小肠结肠炎模型中肠三叶因子对PI3K/AKT/GSK-3β通路影响及意义[J].中华临床医师杂志(电子版),2013,7(12):5392-5396. 6. 王丹, 董红敬, 郝翠,等. 核桃分心木化学成分及抗炎活性研究[J]. 天然产物研究与开发, 2018, 030(009):1548-1553,1607. 7. 宋志强, 丁祥, 朱淼,等. 白僵菌多糖对肿瘤细胞及免疫细胞作用的研究[J]. 现代农业科技, 2019, 741(07):187-188. 8. 苗丰, 董琳琳, 丰晨然,等. 青紫颗粒抑制卵蛋白致过敏性紫癜大鼠血清免疫复合物保护肾组织作用[J]. 中南药学, 2020年18卷5期, 756-760页, ISTIC CA, 2020. 9. 吴雪钗, 王清坪. 黄桑胶囊治疗慢性阻塞性肺疾病模型大鼠机制的初步研究[J]. 华西药学杂志, 2018, 33(3). 10. Fan, Hui-Jie, et al. "Effects and mechanism of action of Huang-Lian-Jie-Du-Tang in atopic dermatitis-like skin dysfunction in vivo and in vitro." Journal of ethnopharmacology 240 (2019): 111937.https://doi.org/10.1016/j.jep.2019.111937 11. Gong Q, Li Y, Ma H, Guo W, Kan X, Xu D, Liu J, Fu S. Peiminine Protects against Lipopolysaccharide-Induced Mastitis by Inhibiting the AKT/NF-κB, ERK1/2 and p38 Signaling Pathways. International Journal of Molecular Sciences. 2018; 19(9):2637. https://doi.o 12. Xia Liu, Qin Xu, Liyan Mei, Hang Lei, Quan Wen, Jifei Miao, Huina Huang, Dongfeng Chen, Shaohui Du, Saixia Zhang, Jianhong Zhou, Rudong Deng, Yiwei Li, Chun Li, Hui Li, Paeonol attenuates acute lung injury by inhibiting HMGB1 in lipopolysaccharide-induced 13. Tang, Ze-yong, et al. "Lycium barbarum polysaccharide alleviates nonylphenol exposure induced testicular injury in juvenile zebrafish." International journal of biological macromolecules 104 (2017): 618-623.https://doi.org/10.1016/j.ijbiomac.2017.06.035 14. Jiang, Kangfeng, et al. "Geraniol alleviates LPS-induced acute lung injury in mice via inhibiting inflammation and apoptosis." Oncotarget 8.41 (2017): 71038.https://dx.doi.org/10.18632%2Foncotarget.20298 15. Zhang, Xu, et al. "Effects of Lycium barbarum polysaccharides on immunological parameters, apoptosis, and growth performance of Nile tilapia (Oreochromis niloticus)." Fish & shellfish immunology 97 (2020): 509-514.https://doi.org/10.1016/j.fsi.2019.12.068 16. Liu, Run, Zhidian Wu, and Hang Yu. "Effect of different treatments on macrophage differentiation in chronic obstructive pulmonary disease and repeated pulmonary infection." Saudi Journal of Biological Sciences 27.8 (2020): 2076-2081.https://doi.org/10.1016 17. Minqian Zhu, Riming Huang, Peng Wen, Ya Song, Baolin He, Jialing Tan, Huili Hao, Hong Wang, Structural characterization and immunological activity of pectin polysaccharide from kiwano (Cucumis metuliferus) peels, Carbohydrate Polymers, Volume 254, 2021, 11 18. [IF=7.711] Zhen Zhang et al."Mitochondria-Accessing Ratiometric Fluorescent Probe for Imaging Endogenous Superoxide Anion in Live Cells and Daphnia magna."Acs Sensors. 2018;3(3):735–741 19. [IF=14.588] Renfa Liu et al."Bioluminescence Imaging of Inflammation in Vivo Based on Bioluminescence and Fluorescence Resonance Energy Transfer Using Nanobubble Ultrasound Contrast Agent."Acs Nano. 2019;13(5):5124–5132 20. [IF=9.381] Minqian Zhu et al."Structural characterization and immunological activity of pectin polysaccharide from kiwano (Cucumis metuliferus) peels."Carbohyd Polym. 2021 Feb;254:117371 21. [IF=9.381] Qing-Chi Wang et al."Structural characterization and immunostimulatory activity in vitro of a glycogen from sea urchin–Strongylocentyotus internedius."Carbohyd Polym. 2021 Apr;258:117701 22. [IF=9.229] Xiaoyun Li et al."Near-Infrared Light-Driven Multifunctional Tubular Micromotors for Treatment of Atherosclerosis."Acs Appl Mater Inter. 2021;13(26):30930–30940 23. [IF=7.46] Shufen Li et al."A lipid droplets specific probe for imaging of atherosclerosis and fibrocalcific bicuspid aortic valves."Sensor Actuat B-Chem. 2021 Nov;346:130458 24. [IF=6.953] Jing Qin et al."Structural characterization and immunoregulatory activity of two polysaccharides from the rhizomes of Atractylodes lancea (Thunb.) DC."Int J Biol Macromol. 2019 Sep;136:341 25. [IF=6.953] Xin Chen et al."Effects of ultrasound-assisted extraction on antioxidant activity and bidirectional immunomodulatory activity of Flammulina velutipes polysaccharide."Int J Biol Macromol. 2019 Nov;140:505 26. [IF=6.953] QingChi Wang et al."Structural characteristics and immune-enhancing activity of an extracellular polysaccharide produced by marine Halomonas sp. 2E1."Int J Biol Macromol. 2021 Jul;183:1660 27. [IF=6.583] Qinyu Wang et al."Naringenin attenuates nonalocholic fatty liver disease by downregulating NLRP3/NF‐κB pathway in mice."Brit J Pharmacol. 2020 Apr;177(8):1806-1821 28. [IF=6.331] Shufen Li et al."Turn-on fluorescent probe for lipid droplet specific imaging of fatty liver and atherosclerosis."J Mater Chem B. 2021 May;9(19):4050-4055 29. [IF=6.331] Yangyang Huang et al."Platelet-derived nanomotor coated balloon for atherosclerosis combination therapy."J Mater Chem B. 2020 Jul;8(26):5765-5775 30. [IF=5.396] Zhiyong Mu et al."Probiotic yeast BR14 ameliorates DSS-induced colitis by restoring the gut barrier and adjusting the intestinal microbiota."Food Funct. 2021 Sep;12(18):8386-8398 31. [IF=5.279] Fang Wu et al."In Vitro and In Vivo Studies Reveal that Hesperetin-7-O-glucoside, a Naturally Occurring Monoglucoside, Exhibits Strong Anti-inflammatory Capacity."J Agr Food Chem. 2021;69(43):12753–12762 32. [IF=5.279] Qian Yang et al."Isolation, Identification, and Immunomodulatory Effect of a Peptide from Pseudostellaria heterophylla Protein Hydrolysate."J Agr Food Chem. 2020;68(44):12259–12270 33. [IF=4.581] Xin-Yan Shang-Guan et al."A C-type lectin with a single CRD from Onychostoma macrolepis mediates immune recognition against bacterial challenge."Fish Shellfish Immun. 2021 Aug;115:160 34. [IF=4.407] Xin-Yan Shang-Guan et al."A C-type lectin (OmCTL) in Onychostoma macrolepis: Binding ability to LPS, PGN and agglutinating activity against bacteria."Mol Immunol. 2021 Apr;132:21 35. [IF=4.36] Hui-Jie Fan et al."Effects and mechanism of action of Huang-Lian-Jie-Du-Tang in atopic dermatitis-like skin dysfunction in vivo and in vitro."J Ethnopharmacol. 2019 Aug;240:111937 36. [IF=4.36] Song Yang et al."Ginseng root extract attenuates inflammation by inhibiting the MAPK/NF-κB signaling pathway and activating autophagy and p62-Nrf2-Keap1 signaling in vitro and in vivo."J Ethnopharmacol. 2021 Oct;:114739 37. [IF=4.321] Shurong Zhou et al."Thiolated Nanoparticles Overcome the Mucus Barrier and Epithelial Barrier for Oral Delivery of Insulin."Mol Pharmaceut. 2020;17(1):239–250 38. [IF=4.219] Run Liu et al."Effect of different treatments on macrophage differentiation in chronic obstructive pulmonary disease and repeated pulmonary infection."Saudi J Biol Sci. 2020 Aug;27:2076 39. [IF=4.098] Zengjin Liu et al."A far-red-emitting fluorescence probe for selective and sensitive detection of no in live cells and in C. elegans."Spectrochim Acta A. 2021 Nov;261:120030 40. [IF=4.073] Qingchi Wang et al."Proteoglycan from Bacillus sp. BS11 Inhibits the Inflammatory Response by Suppressing the MAPK and NF-κB Pathways in Lipopolysaccharide-Induced RAW264.7 Macrophages."Mar Drugs. 2020 Dec;18(12):585 41. [IF=3.968] Minghui Li et al."A biomimetic antitumor nanovaccine based on biocompatible calcium pyrophosphate and tumor cell membrane antigens."Asian J Pharm Sci. 2021 Jan;16:97 42. [IF=3.932] Bo Cao et al."Protective effects of notoginsenoside R1 on acute lung injury in rats with sepsis."Ann Transl Med. 2021 Jun; 9(12): 996 43. [IF=3.775] Qian Yang et al."Investigation on activation in RAW264.7 macrophage cells and protection in cyclophosphamide-treated mice of Pseudostellaria heterophylla protein hydrolysate."Food Chem Toxicol. 2019 Dec;134:110816 44. [IF=3.101] Huili Hao et al."Chemical composition and immunostimulatory properties of green alga Caulerpa racemosa var peltata."Food Agr Immunol. 2019;30(1):937-954 45. [IF=2.448] Xin Song et al."Comprehensive transcriptome analysis based on RNA sequencing identifies critical genes for lipopolysaccharide-induced epididymitis in a rat model."Asian J Androl. 2019 Nov-Dec; 21(6): 605–611 46. [IF=4.932] Yingchun Su et al."Hordenine inhibits neuroinflammation and exerts neuroprotective effects via inhibiting NF-κB and MAPK signaling pathways in vivo and in vitro."Int Immunopharmacol. 2022 Jul;108:108694 47. [IF=2.952] Lulu Song et al."Fxyd5 activates the NF‑κB pathway and is involved in chondrocytes inflammation and extracellular matrix degradation."Mol Med Rep. 2022 Apr;25(4):1-8 48. [IF=15.881] Ziyu Wu et al."Carrier-Free Trehalose-Based Nanomotors Targeting Macrophages in Inflammatory Plaque for Treatment of Atherosclerosis."Acs Nano. 2022;XXXX(XXX):XXX-XXX 49. [IF=3.738] Jing Hongyuan et al."LncRNAs Transcriptome Analysis Revealed Potential Mechanisms of Selenium to Mastitis in Dairy Cows."Biol Trace Elem Res. 2022 Jan;:1-9 50. [IF=6.023] Meitong Liu et al."Alliin alleviates LPS-induced pyroptosis via promoting mitophagy in THP-1 macrophages and mice."Food Chem Toxicol. 2022 Feb;160:112811 51. [IF=3.636] Hongzhou Xu et al."Molecular characterization and antibacterial ability of galectin-3 and galectin-9 in Onychostoma macrolepis."Dev Comp Immunol. 2022 Mar;128:104333 52. [IF=8.128] Yingfang Tao et al."Nitric oxide-driven nanomotors with bowl-shaped mesoporous silica for targeted thrombolysis."J Colloid Interf Sci. 2021 Dec;: 53. [IF=13.281] Ziyu Wu et al."Multi-Pathway Microenvironment Regulation for Atherosclerosis Therapy Based on Beta-Cyclodextrin/L-Arginine/Au Nanomotors with Dual-Mode Propulsion."Small. 2021 Dec 16 54. [IF=4.412] Xue Li et al."Isoquercitrin Attenuates Osteogenic Injury in MC3T3 Osteoblastic Cells and the Zebrafish Model via the Keap1-Nrf2-ARE Pathway."MOLECULES. 2022 Jan;27(11):3459 55. [IF=5.182] Yulin Wu et al."A new polysaccharide from Caulerpa chemnitzia induces molecular shifts of immunomodulation on macrophages RAW264.7."Food Chemistry-X. 2022 Jun;14:100313 |
脂多糖 - 介绍
脂多糖，英文名称Lipopolysaccharides，简称LPS，是革兰氏阴性细菌细胞壁中的一种特有成分，位于细胞壁的最外层并暴露于非荚膜细菌的细胞表面，有利于维持细胞外膜的完整性，保护细菌免受胆汁盐和脂类抗生素的破坏。结构上，脂多糖由类脂A、核心多糖和O-多糖侧链组成。其中类脂质A是构成细菌内毒素的主要成分，决定其毒性强弱；而O-多糖侧链在不同细菌间是高度变化的，特异性决定细菌的血清型。LPS可以引起免疫刺激的级联反应和机体的毒性病理生理活动，包括释放内毒素引起感染性休克从而导致末梢血管虚脱。临床常通过检测LPS的存在来诊断脑膜炎。正是LPS与机体免疫机能的密切关系，生命科学研究常常提取LPS进行相关的研究，如阐明LPS的结构，代谢，免疫学，生理学，毒性，生物合成途径；诱导生长促进因子如白介素的合成与分泌；诱导疾病研究的动物模型如炎症反应，急性肺损伤。将LPS分别与FITC、TRITC或2,4,6-trinitrobenzene sulfonic acid (TNBSA)反应即可得到FITC、TRITC或TNP标记的LPS，主要用于非胸腺依赖性B细胞对细菌LPS的免疫应答研究。。LPS可通过TCA、酚、酚-氯仿-石油醚等方法制备，其中最后一种方法为粗提取法。TCA和酚提取得到的LPS在结构上、电泳图谱和内毒素水平均较相似，不同之处在于含有的核酸和蛋白质残留量不同，TCA法得到的LPS含有约2%的RNA和10%的变性蛋白。酚法提取的LPS含有达60%的RNA和少于1%的蛋白。用凝胶过滤层析可除去酚提取LPS中的残留蛋白，但是仍会留下约10-20%的核酸。进一步用离子交换层析法纯化可得到＜1%蛋白和＜1%RNA的LPS。本品来源于血清型大肠杆菌O55:B5，用苯法提取而得。其内毒素水平不少于500,000 EU (endotoxin units)/mg，经分析，1ng相当于0.5EU（鲎试剂法）和10EU（显色法）。LPS O55:B5可用于刺激人腹腔巨噬细胞（1ng/ml）以及马腹腔巨噬细胞的活动（1-100ng/ml）。
最后更新：2022-10-16 17:31:03
脂多糖 - 用途与合成方法
脂多糖是一种酰胺多糖类化合物，具有多种用途和应用。脂多糖可以用于制备各种材料，如涂料、纸张、液晶屏、荧光染料、聚合物材料等，具有较好的稳定性和生物相容性。
脂多糖的合成方法有多种，常见的方法包括酰胺化反应、胺化反应和酯化反应。
酰胺化反应是将脂肪酸和胺在适当的溶剂中反应，生成脂多糖。该方法需要有机酸催化剂和适当的温度条件。
胺化反应是将脂肪酸的羧基与氨反应生成脂多糖。该反应需要碱催化剂和适当的温度和压力条件。
酯化反应是将脂肪酸酯与胺反应生成脂多糖。该方法需要酸催化剂和适当的温度条件。
这些合成方法都可以根据具体的需求进行优化和改良，以得到所需的脂多糖化合物。脂多糖的合成方法选择应根据反应物的性质、目标产物的要求和实际操作的可行性来确定。
脂多糖的合成方法有多种，常见的方法包括酰胺化反应、胺化反应和酯化反应。
酰胺化反应是将脂肪酸和胺在适当的溶剂中反应，生成脂多糖。该方法需要有机酸催化剂和适当的温度条件。
胺化反应是将脂肪酸的羧基与氨反应生成脂多糖。该反应需要碱催化剂和适当的温度和压力条件。
酯化反应是将脂肪酸酯与胺反应生成脂多糖。该方法需要酸催化剂和适当的温度条件。
这些合成方法都可以根据具体的需求进行优化和改良，以得到所需的脂多糖化合物。脂多糖的合成方法选择应根据反应物的性质、目标产物的要求和实际操作的可行性来确定。
最后更新：2024-04-10 22:29:15
供应商列表
您刚刚浏览过
你知道吗？
微信搜索化工百科或扫描下方二维码，添加化工百科小程序，随时随地查信息！