荧光素钾
D-LUCIFERIN K SALT(D-LUCIFERIN POTASSIUM SALT)
CAS: 115144-35-9
化学式: C11H7KN2O3S2
| 中文名 | D-LUCIFERIN K SALT |
| 英文名 | D-LUCIFERIN POTASSIUM SALT |
| 别名 | 荧光素钾 D-荧光素钾 D-荧光素钾盐 D-LUCIFERIN POTASSIUM SALT D-虫荧光素钾盐 (S)-4,5-二氢-2-(6-羟基苯并噻唑-2-基)噻唑-4-甲酸钾盐 D-荧光素钾盐POTASSIUM (S)-2-(6-HYDROXYBENZO[D]THIAZOL-2-YL)-4,5-DIHYDROTHIAZOLE-4-CARBOXYLATE |
| 英文别名 | D-LUCIFERIN K SALT D-Luciferenpotassiumsalt D-LUCIFERIN POTASSIUM SALT D-LUCIFERIN, MONOPOTASSIUM SALT BEETLE LUCIFERIN, POTASSIUM SALT D-LUCIFERIN FIREFLY, POTASSIUM SALT D -LUCIFERIN SYNTHETIC POTASSIUM SALT 4,5-DIHYDRO-2-(6-HYDROXY-2-BENZOTHIAZOLYL)-4-THIAZOLECARBOXYLIC ACID POTASSIUM SALT D-(-)-2-[6'-HYDROXY-2'-BENZOTHIAZOLYL]DELTA-THIAZOLINE-4-CARBOXYLIC ACID POTASSIUM SALT potassium (4S)-2-(6-hydroxy-1,3-benzothiazol-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylate |
| CAS | 115144-35-9 |
| EINECS | 1592732-453-0 |
| 化学式 | C11H7KN2O3S2 |
| 分子量 | 318.41 |
| InChI | InChI=1/C11H8N2O3S2.K/c14-5-1-2-6-8(3-5)18-10(12-6)9-13-7(4-17-9)11(15)16;/h1-3,7,14H,4H2,(H,15,16);/q;+1/p-1/t7-;/m1./s1 |
| InChIKey | UMBKGTQQGYPQBE-OGFXRTJISA-M |
| 溶解度 | 水: 可溶 |
| 存储条件 | Inert atmosphere,Store in freezer, under -20°C |
| 外观 | 粉末或晶体 |
| BRN | 5416262 |
| MDL号 | MFCD00044928 |
| 体外研究 | D-luciferin is the natural substrate of the enzyme luciferase (Luc), that catalyzes the production of the typical yellowgreen light of fireflies.The present review covers the synthesis of D-luciferin and derivatives or analogues that are substrates or inhibitors of the luciferase from the American firefly Photinus pyralis , the enzyme more frequently used in techniques of in vitro and optical imaging. D-Luciferin exhibits a decrease in the measured K m in PC3M-Luc cell lysates with a K m of 34 μM. |
| 体内研究 | Bioluminescence imaging (BLI) using the firefly luciferase (Fluc) as a reporter gene and D-luciferin as a substrate is currently the most widely employed technique. The total signal intensity is plotted against the time after D-luciferin injection to generate a time-intensity curve. In addition to the peak signal, the signals at fixed time points (5, 10, 15, and 20 min) after D-luciferin injection are determined as alternatives to the peak signal. The signal in a given time-intensity curve is normalized for the peak signal in the curve to represent the pattern of temporal changes after D-luciferin injection. Inject with 10 μL of D-luciferin (intraperitoneally or intravenously) stock solution per gram of body weight: normally ~200 μL for a 20 g mouse for a standard 150 mg/kg injection. Thaw D-Luciferin (either Potassium or Sodium Salt) at room temperature and dissolve in dPBS (no calcium or magnesium) to a final concentration of 15 mg/mL. Pre-wet a 0.22 μm filter by drawing through 5-10 mL of sterile H 2 O and discard water. Sterilize the D-Luciferin solution through the prepared 0.22 μm syringe filter. |
| WGK Germany | 3 |
| 海关编号 | 29342000 |
| 参考资料 展开查看 | 1. [IF=8.456] Chuchu Zhou et al."Alternative and Injectable Preformed Albumin-Bound Anticancer Drug Delivery System for Anticancer and Antimetastasis Treatment."Acs Appl Mater Inter. 2019;11(45):42534–42548 |
荧光素钾 - 简介
D-LUCIFERIN K SALT是一种化学物质，具有以下性质：
物理性质：D-LUCIFERIN K SALT为白色固体，溶于水和有机溶剂。
用途：D-LUCIFERIN K SALT常用于生物荧光成像技术中。它是一种发光底物，可以和荧光酶（如荧光素酶）发生反应，产生强烈的荧光信号，用于研究生物系统的活性、代谢和功能。
制法：D-LUCIFERIN K SALT的制备方法可以通过化学合成或生物合成来实现。化学合成通常使用已知原料和合成路线，而生物合成则利用生物体内的代谢途径进行合成。
安全信息：D-LUCIFERIN K SALT在常规使用下相对安全，但仍需注意以下事项：避免吸入或接触皮肤、眼睛和粘膜。在操作时需佩戴适当的防护手套、眼镜和防护服。应在通风良好的地方操作，并将其储存在干燥、避光和通风的地方。如有不慎吸入或接触，应立即清洗受影响的区域，并就医咨询。
物理性质：D-LUCIFERIN K SALT为白色固体，溶于水和有机溶剂。
用途：D-LUCIFERIN K SALT常用于生物荧光成像技术中。它是一种发光底物，可以和荧光酶（如荧光素酶）发生反应，产生强烈的荧光信号，用于研究生物系统的活性、代谢和功能。
制法：D-LUCIFERIN K SALT的制备方法可以通过化学合成或生物合成来实现。化学合成通常使用已知原料和合成路线，而生物合成则利用生物体内的代谢途径进行合成。
安全信息：D-LUCIFERIN K SALT在常规使用下相对安全，但仍需注意以下事项：避免吸入或接触皮肤、眼睛和粘膜。在操作时需佩戴适当的防护手套、眼镜和防护服。应在通风良好的地方操作，并将其储存在干燥、避光和通风的地方。如有不慎吸入或接触，应立即清洗受影响的区域，并就医咨询。
最后更新：2024-04-09 15:16:47
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