| properties | legone is hops resin a-picric acid, which is a hypha in the water of an umbel. it will undergo related chemical reactions when exposed to oxygen and water to generate other substances, and the higher the ambient temperature, the faster the reaction speed. |
| effect | through in vivo and in vitro pharmacological studies, it was found and proved that rutone can significantly promote the proliferation of osteoblasts, increase the levels of serum osteocalcin (BGP) and alkaline phosphatase (AKP) in retinoic acid-induced osteoporosis rats, and reduce the expression of tartrate-resistant acid phosphatase (TRACP). |
| preparation | 700g of crushed hops are loaded into the extractor, and appropriate extraction pressure, extraction temperature, CO2 flow rate and extraction time are selected. CO2 enters the extractor through cooling and pressurization. under heating and pressurization, the effective components in hops are dissolved in CO2 in liquid or supercritical state and pass through the separator with CO2 fluid. By reducing the pressure and increasing the temperature, the active ingredient and CO2 are converted into a gaseous state and separated from the separator, while the extract remains in the separator, and the separated CO2 gas is condensed and recycled. After the extraction time is reached, stop the operation and take the sample from the sample receiving valve at the bottom of the separator. When the system pressure returns to normal pressure, the hop extract can be removed for the next operation. The hop extract residue in the extractor was collected for subsequent experiments.
weigh 100mg of hops supercritical CO2 resin extract in a 10mL volumetric flask, dissolve methanol and fix the volume to scale. Mobile phase: methanol (A): 0.1% phosphoric acid (B)= 90: 10; Flow rate: 0.8 mL/min; Injection volume: 10 μL; Column temperature: 25 ℃; Detection time: 20min; Detection wavelength: 315nm. Separation and purification to obtain rvxone. |
