1047953-91-2

1047953-91-2 - Names and Identifiers

Name	2-chloro-N-(4-(5-(3,4-dichlorophenyl)-3-(2-methoxyethoxy)-1H-1,2,4-triazol-1-yl)phenyl)acetamide
Synonyms	MI-2 CHD-4 MALT1 inhibitor MI-2 MI 2 (MALT1 inhibitor) TELOMERASE INHIBITOR II, SODIUM SALT 5'-D(ATGAAAATCAGGGTTAGG)-3', SODIUM SALT 2-chloro-N-(4-(5-(3,4-dichlorophenyl)-3-(2-methoxyethoxy)-1H-1,2,4-triazol-1-yl)phenyl)acetamide 2-Chloro-N-[4-[5-(3,4-dichlorophenyl)-3-(2-methoxyethoxy)-1H-1,2,4-triazol-1-yl]phenyl]-Acetamide
CAS	1047953-91-2

1047953-91-2 - Physico-chemical Properties

Molecular Formula	C19H17Cl3N4O3
Molar Mass	455.72
Solubility	DMSO: ≥ 46 mg/mL mother liquor preservation: sub-package and freeze storage to avoid repeated freezing and thawing;-20 ℃,1 month;-80 ℃,6 months (after dilution, the solution temperature is low and storage may precipitate, as far as possible to use the current preparation) Cell experiment: dissolve with DMSO first: dilute with culture medium then, and the dilution process is recommended to be carried out in stages to avoid too fast concentration change leading to compound precipitation. If the compound is precipitated during the dilution process, it can be redissolved by ultrasound. During dilution, ensure that the final concentration of DMSO in the working fluid should be below 0.1% as far as possible, and the maximum should not exceed 0.5%, and set up a DMSO control group with corresponding concentration. Animal experiment: Dissolve with DMSO first: dilute with water or normal saline, etc. The dilution process is recommended to be carried out in sections to avoid excessive concentrat
Storage Condition	-20°C
Use	MI-2 MALT1 inhibitor, also known as MI-2, is a MALT1 inhibitor (IC50 = 5.84 μM). MI-2 binds directly to MALT1 and irreversibly suppresses protease function. Decreases NF-κB activity induced by MALT1. Mucosa-associated-lymphoid-tissue lymphoma-translocation gene 1 (MALT1), a paracaspase and essential regulator for nuclear factor kB (NF-κB) activation, plays an important role in innate and adaptive immunity. Suppression of MALT1 protease activity with small molecule inhibitors showed promising efficacies in subtypes of B cell lymphoma and improvement in experimental autoimmune encephalomyelitis model.
Target	MALT1

1047953-91-2 - Reference

Reference

1: Fusco R, Siracusa R, D'Amico R, Cordaro M, Genovese T, Gugliandolo E, Peritore AF, Crupi R, Di Paola R, Cuzzocrea S, Impellizzeri D. Mucosa-Associated Lymphoid Tissue Lymphoma Translocation 1 Inhibitor as a Novel Therapeutic Tool for Lung Injury. Int J Mol Sci. 2020 Oct 20;21(20):7761. doi: 10.3390/ijms21207761. PMID: 33092214; PMCID: PMC7589767.
2: Wang R, Zhang H, Xu J, Zhang N, Pan T, Zhong X, Zhang H, Yin L, Yao Y, Wu Q, Li Z, Liu X, Xu K, Niu M. MALT1 Inhibition as a Therapeutic Strategy in T-Cell Acute Lymphoblastic Leukemia by Blocking Notch1-Induced NF-κB Activation. Front Oncol. 2020 Sep 23;10:558339. doi: 10.3389/fonc.2020.558339. PMID: 33072583; PMCID: PMC7538650.
3: Ishikawa C, Mori N. MALT-1 as a novel therapeutic target for adult T-cell leukemia. Eur J Haematol. 2020 Oct;105(4):460-467. doi: 10.1111/ejh.13467. Epub 2020 Jul 24. PMID: 32574386.
4: Liu X, Yue C, Shi L, Liu G, Cao Q, Shan Q, Wang Y, Chen X, Li H, Wang J, Gao S, Niu M, Yu R. MALT1 is a potential therapeutic target in glioblastoma and plays a crucial role in EGFR-induced NF-κB activation. J Cell Mol Med. 2020 Jul;24(13):7550-7562. doi: 10.1111/jcmm.15383. Epub 2020 May 25. PMID: 32452133; PMCID: PMC7339184.
5: Izumi K, Nishikori M, Yuan H, Otsuka Y, Nakao K, Takaori-Kondo A. Establishment and characterization of a MALT lymphoma cell line carrying an API2-MALT1 translocation. Genes Chromosomes Cancer. 2020 Sep;59(9):517-524. doi: 10.1002/gcc.22855. Epub 2020 May 6. PMID: 32348592.
6: Dezorella N, Ashkenazi

1047953-91-2 - Preparation solution concentration reference

	1mg	5mg	10mg
1 mM	2.194 ml	10.972 ml	21.943 ml
5 mM	0.439 ml	2.194 ml	4.389 ml
10 mM	0.219 ml	1.097 ml	2.194 ml
5 mM	0.044 ml	0.219 ml	0.439 ml

Last Update：2024-01-02 23:10:35

1047953-91-2 - Cell Experiment

Cell proliferation is determined by ATP quantification using a luminescent method and trypan blue dye exclusion. Standard curves for each cell line are calculated by plotting the cell number (determined using trypan blue) against their luminescence values, and cell number is calculated accordingly. Cell viability in drug-treated cells is normalized to their respective controls (fractional viability), and results are given as 1-fractional viability. CompuSyn software is used to determine GI25 and GI50 values.(Only for Reference) Cell lines: MALT1-independent cell lines: U2932 and HLY-1, and the two GCB-DLBCL cell lines; MALT1-dependent cell lines: HBL-1, TMD8, OCI-Ly3, and OCI-Ly10 cells.

Last Update：2023-08-16 21:32:38