Name | Fluorescein (Solvent Yellow 94) |
Synonyms | Fluorescein (Solvent Yellow 94) Fluorescein (Solvent Yellow 94) 518-45-6 3',6'-dihydroxy-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one |
CAS | 126605-73-0 |
Molecular Formula | C20H12O5 |
Molar Mass | 332.30628 |
properties | Fluorescein (English: Fluorescein, also known as Fluorescein) is a kind of synthetic organic compounds, appearance is dark orange/red powder, soluble in ethanol, slightly soluble in water. Fluorescent yellow is also called "fluorescent orange red". Scientific name "9-(2 '-carboxyphenyl)-6-hydroxy-3h-xantho-3-one". Formula C20H12O5. Molecular weight 332.31. Orange-yellow or orange-red crystalline powder. Soluble in alcohol, acetic acid, sodium hydroxide solution, insoluble in water, ether, chloroform and benzene. The anion is dissociated in the solution to make the solution yellow-green and fluorescent. It is mainly used as an adsorption indicator. Commonly used in silver nitrate titration of chloride ion, bromide ion and iodide ion process, when used, the pH range of the solution is 7~10. |
production process | Industrial fluorescein is generally derived from resorcinol and phthalic anhydride heat condensation, which contains a large number of impurities, there are mainly unreacted resorcinol, phenolphthalein and acridine yellow. The traditional method of purifying fluorescein is to dissolve fluorescein in alkali solution, adsorb impurities with activated carbon, and then acidify and precipitate fluorescein, the process of alkali dissolution, acidification, and filtration washing was then repeated several times. |