1493694-70-4

1493694-70-4 - Names and Identifiers

Name	(1r,4r)-4-((2-(Butylamino)-5-(5-(morpholinomethyl)pyridin-2-yl)pyrimidin-4-yl)amino)cyclohexanol
Synonyms	UNC2250 CS-1346 UNC2250(UNC 2250) 4-[[2-(butylamino)-5-[5-(morpholin-4-ylmethyl)pyridin-2-yl]pyrimidin-4-yl]amino]cyclohexan-1-ol (1r,4r)-4-((2-(Butylamino)-5-(5-(morpholinomethyl)pyridin-2-yl)pyrimidin-4-yl)amino)cyclohexanol (1r,4r)-4-((2-(butylamino)-5-(5-(morpholinomethyl)pyridin-2-yl)pyrimidin-4-yl)amino)cyclohexanol trans-4-[[2-(Butylamino)-5-[5-(4-morpholinylmethyl)-2-pyridinyl]-4-pyrimidinyl]amino]cyclohexanol Cyclohexanol, 4-[[2-(butylamino)-5-[5-(4-morpholinylmethyl)-2-pyridinyl]-4-pyrimidinyl]amino]-, trans- trans-4-[[2-(Butylamino)-5-[5-(4-morpholinylmethyl)-2-pyridinyl]-4-pyrimidinyl]amino]cyclohexanol UNC2250
CAS	1493694-70-4

1493694-70-4 - Physico-chemical Properties

Molecular Formula	C24H36N6O2
Molar Mass	440.58
Density	1.224±0.06 g/cm3(Predicted)
Melting Point	207-210oC
Boling Point	657.4±65.0 °C(Predicted)
Solubility	Chloroform (Slightly, Heated), Ethyl Acetate (Slightly, Heated), Methanol (Sligh
Appearance	Solid
Color	Pale Yellow to Yellow
pKa	15.02±0.40(Predicted)
Storage Condition	Refrigerator, under inert atmosphere
Use	UNC2250 is a potent selective Mer inhibitor with IC50 of 1.7 nM, which is 160 and 60 times more selective than the highly correlated enzyme Axl/Tyro3. Kinase experiment: microfluidic capillary electrophoresis (MCE) analysis test: activity determination was carried out in a 384-well polypropylene microplate. At Km, 50 mM Hepes with a final volume of 50 μL, pH 7.4, containing 10 mM MgCl2, 1.0 mM DTT, 0.01% Triton X-100, 0.1% bovine serum protein (BSA), containing 1.0 μM fluorescent matrix and ATP were used for each enzyme. All reactions were terminated by the addition of 20 μL of 70 mM EDTA. After 180 minutes of incubation, the phosphorylated and non-phosphorylated basal peptides were separated in a buffer and supplemented with 1 × CR-8 on a LabChip EZ reader equipped with a 12- sipper chip. Data analysis was performed using EZ reading software.
In vitro study	UNC2250 effectively inhibited phosphorylation of Mer in 697 B- ALL cells and Colo699 NSCLC cells. In addition, UNC2250 may also exhibit functional anti-tumor activity by reducing the potential for colony formation in Rod tumor cells and NSCLC cells.
In vivo study	Based on in vivo PK experiments, UNC2250 has moderate Half-Life, clearance and volume of distribution as well as reasonable oral bioavailability and good solubility.

1493694-70-4 - Preparation solution concentration reference

	1mg	5mg	10mg
1 mM	2.27 ml	11.349 ml	22.697 ml
5 mM	0.454 ml	2.27 ml	4.539 ml
10 mM	0.227 ml	1.135 ml	2.27 ml
5 mM	0.045 ml	0.227 ml	0.454 ml

Last Update：2024-01-02 23:10:35

1493694-70-4 - Cell Experiment

Cell lines: BT-12 Rod tumor cells, and Colo699 NSCLC cells concentration: BT-12 ~ 3 μm, Colo699 ~ 300 nm incubation Time: 3 weeks BT-12, Colo699 2 weeks Method: BT-12 rod-shaped tumor cells (10000 cells) were cultured in 2.0 0.35% soft agar containing 0.5 x RPMI medium, 7.5% FBS at the indicated concentration with inhibitor or DMSO vector only, and 0.5 of 1 ×rpmi medium containing 15% FBS and inhibitor or DMSO-only vector was covered. Medium and inhibitor or carrier were changed twice a week. Colonies were stained with thiazolyl blue tetrazolium bromide and counting began after three weeks. Colo699 NSCLC cells (15000 cells) were cultured in 1.5 0.35% soft agar containing 1 ×rpmi medium and 10% FBS, and 2.0 mL of 1 ×rpmi medium containing 10% FBS and indicated concentration containing inhibitor or DMSO vector only was covered. Medium and inhibitor or carrier were changed three times per week. Colonies were stained with nitrotetrazolium blue chloride and counting began after two weeks.

Last Update：2023-08-16 21:32:38

1493694-70-4 - Introduction

(1r,4r)-4-((2-(Butylamino)-5-(5-(morpholinomethyl)pyridin-2-yl)pyrimidin-4-yl)amino)cyclohexanol is a chemical substance, it is a protein methylase inhibitor. It is mainly used to treat cancer and some other diseases.

(1r,4r)-4-((2-(Butylamino)-5-(5-(morpholinomethyl)pyridin-2-yl)pyrimidin-4-yl)amino) the preparation method of cyclohexanol is mainly prepared by organic synthesis method. The specific preparation method may include the steps of synthesis reaction, solvent extraction and crystallization. The details of the preparation may vary depending on the experimental conditions of the researcher.

Safety information for (1r,4r)-4-((2-(Butylamino)-5-(5-(morpholinomethyl)pyridin-2-yl)pyrimidin-4-yl)amino)cyclohexanol, need to pay attention to the following points:
1.(1r,4r)-4-((2-(Butylamino)-5-(5-(morpholinomethyl)pyridin-2-yl)pyrimidin-4-yl)amino)cyclohexanol is a chemical reagent, relevant safety practices should be followed, including the wearing of appropriate personal protective equipment.
2.(1r,4r)-4-((2-(Butylamino)-5-(5-(morpholinomethyl)pyridin-2-yl)pyrimidin-4-yl)amino)cyclohexanol may be toxic to humans, may have adverse health effects. Therefore, when using (1r,4r)-4-((2-(Butylamino)-5-(5-(morpholinomethyl)pyridin-2-yl)pyrimidin-4-yl)amino) adequate assessment and risk analysis should be performed prior to cyclohexanol.
3. Need to use (1r,4r)-4-((2-(Butylamino)-5-(5-(morpholinomethyl)pyridin-2-yl)pyrimidin-4-yl)amino) in a well-ventilated laboratory environment cyclohexanol, and avoid direct contact with mouth, eyes and skin.
4. If accidental exposure to (1r,4r)-4-((2-(Butylamino)-5-(5-(morpholinomethyl)pyridin-2-yl)pyrimidin-4-yl)amino)cyclohexanol, the contaminated area should be cleaned immediately and medical assistance should be sought if necessary.
5.(1r,4r)-4-((2-(Butylamino)-5-(5-(morpholinomethyl)pyridin-2-yl)pyrimidin-4-yl)amino)cyclohexanol needs to be stored in a dry, dark, low temperature places, and away from fire and flammable materials.

Last Update：2024-04-09 20:48:19